DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions trini Trichoplusia ni (Cabbage looper) Cabbage looper x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216209X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216206X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216210X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216213X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216207X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216211X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216212X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216208X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. Nature 2010 imex curation Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hatzivassiliou G., Song K., Yen I., Brandhuber BJ., Anderson DJ., Alvarado R., Ludlam MJ., Stokoe D., Gloor SL., Vigers G., Morales T., Aliagas I., Liu B., Sideris S., Hoeflich KP., Jaiswal BS., Seshagiri S., Koeppen H., Belvin M., Friedman LS., Malek S. DIP original experiment: DIP-216214X Nature 2010 IMEx RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth. braf_human Serine/threonine-protein kinase B-raf BRAF BRAF1 RAFB1 v-Raf murine sarcoma viral oncogene homolog B1 p94 Proto-oncogene B-Raf protein protein human Homo sapiens Human MAALSGGGGGGAEPGQALFNGDMEPEAGAGAGAAASSAADPAIPEEVWNIKQMIKLTQEHIEALLDKFGGEHNPPSIYLEAYEEYTSKLDALQQREQQLLESLGNGTDFSVSSSASMDTVTSSSSSSLSVLPSSLSVFQNPTDVARSNPKSPQKPIVRVFLPNKQRTVVPARCGVTVRDSLKKALMMRGLIPECCAVYRIQDGEKKPIGWDTDISWLTGEELHVEVLENVPLTTHNFVRKTFFTLAFCDFCRKLLFQGFRCQTCGYKFHQRCSTEVPLMCVNYDQLDLLFVSKFFEHHPIPQEEASLAETALTSGSSPSAPASDSIGPQILTSPSPSKSIPIPQPFRPADEDHRNQFGQRDRSSSAPNVHINTIEPVNIDDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNVKALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRIGSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNILLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQGMDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQLSGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRDQIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILASIELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAFPVH DIP protein P15056 original sequence version: 182 DIP protein P15056 original sequence version: 133 DIP protein P15056 original sequence version: 147 DIP protein P15056 original sequence version: 173 DIP protein P15056 original sequence version: 178 DIP protein P15056 original sequence version: 188 DIP protein P15056 original sequence version: 193 DIP protein P15056 original sequence version: 195 DIP protein P15056 original sequence version: 146 DIP protein P15056 original sequence version: 153 0798C2AAB487E813 raf1_human RAF proto-oncogene serine/threonine-protein kinase RAF1 RAF Proto-oncogene c-RAF Raf-1 protein protein human Homo sapiens Human MEHIQGAWKTISNGFGFKDAVFDGSSCISPTIVQQFGYQRRASDDGKLTDPSKTSNTIRVFLPNKQRTVVNVRNGMSLHDCLMKALKVRGLQPECCAVFRLLHEHKGKKARLDWNTDAASLIGEELQVDFLDHVPLTTHNFARKTFLKLAFCDICQKFLLNGFRCQTCGYKFHEHCSTKVPTMCVDWSNIRQLLLFPNSTIGDSGVPALPSLTMRRMRESVSRMPVSSQHRYSTPHAFTFNTSSPSSEGSLSQRQRSTSTPNVHMVSTTLPVDSRMIEDAIRSHSESASPSALSSSPNNLSPTGWSQPKTPVPAQRERAPVSGTQEKNKIRPRGQRDSSYYWEIEASEVMLSTRIGSGSFGTVYKGKWHGDVAVKILKVVDPTPEQFQAFRNEVAVLRKTRHVNILLFMGYMTKDNLAIVTQWCEGSSLYKHLHVQETKFQMFQLIDIARQTAQGMDYLHAKNIIHRDMKSNNIFLHEGLTVKIGDFGLATVKSRWSGSQQVEQPTGSVLWMAPEVIRMQDNNPFSFQSDVYSYGIVLYELMTGELPYSHINNRDQIIFMVGRGYASPDLSKLYKNCPKAMKRLVADCVKKVKEERPLFPQILSSIELLQHSLPKINRSASEPSLHRAAHTEDINACTLTTSPRLPVF DIP protein P04049 original sequence version: 117 DIP protein P04049 original sequence version: 106 DIP protein P04049 original sequence version: 114 DIP protein P04049 original sequence version: 141 DIP protein P04049 original sequence version: 142 DIP protein P04049 original sequence version: 147 DIP protein P04049-1 original sequence version: 136 DIP protein P04049 original sequence version: 136 DIP protein P04049 original sequence version: 153 DIP protein P04049 original sequence version: 150 DIP protein P04049 original sequence version: 164 DIP protein P04049 original sequence version: 172 DIP protein P04049-1 original sequence version: 172 DIP protein P04049 original sequence version: 184 DIP protein P04049 original sequence version: 188 DIP protein P04049 original sequence version: 195 EF821B5349711BC3 araf_human Serine/threonine-protein kinase A-Raf ARAF PKS PKS2 ARAF1 Proto-oncogene A-Raf-1 Proto-oncogene Pks Proto-oncogene A-Raf protein protein human Homo sapiens Human MEPPRGPPANGAEPSRAVGTVKVYLPNKQRTVVTVRDGMSVYDSLDKALKVRGLNQDCCVVYRLIKGRKTVTAWDTAIAPLDGEELIVEVLEDVPLTMHNFVRKTFFSLAFCDFCLKFLFHGFRCQTCGYKFHQHCSSKVPTVCVDMSTNRQQFYHSVQDLSGGSRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKKVKNLGYRDSGYYWEVPPSEVQLLKRIGTGSFGTVFRGRWHGDVAVKVLKVSQPTAEQAQAFKNEMQVLRKTRHVNILLFMGFMTRPGFAIITQWCEGSSLYHHLHVADTRFDMVQLIDVARQTAQGMDYLHAKNIIHRDLKSNNIFLHEGLTVKIGDFGLATVKTRWSGAQPLEQPSGSVLWMAAEVIRMQDPNPYSFQSDVYAYGVVLYELMTGSLPYSHIGCRDQIIFMVGRGYLSPDLSKISSNCPKAMRRLLSDCLKFQREERPLFPQILATIELLQRSLPKIERSASEPSLHRTQADELPACLLSAARLVP DIP protein P10398 original sequence version: 126 DIP protein P10398 original sequence version: 168 D23E5711304AA468 raf1-braf-5 6 10 unspecified role unspecified role prey prey Kinase domain Region 457-717 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction raf1-braf-8 4 11 unspecified role unspecified role bait bait Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role prey prey mCherry-tag mCherry-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association araf-braf-1 2 12 unspecified role unspecified role bait bait human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human association association raf1-1 1 11 unspecified role unspecified role unspecified role unspecified role Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction raf1-braf-18 7 10 unspecified role unspecified role prey prey Kinase domain Region 457-717 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction raf1-braf-20 9 11 unspecified role unspecified role bait bait Venus-tag Venus-tag fluorescent prot tag fluorescent protein tag fluorescent prot tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association raf1-braf-21 5 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association raf1-raf1-3 3 11 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range trini Trichoplusia ni (Cabbage looper) Cabbage looper 11 unspecified role unspecified role prey prey Kinase domain Region 324-618 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain trini Trichoplusia ni (Cabbage looper) Cabbage looper direct interaction direct interaction braf-raf1-23 8 10 unspecified role unspecified role bait bait human Homo sapiens Human 11 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association