DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. DIP original experiment: DIP-215860X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. DIP original experiment: DIP-215856X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. DIP original experiment: DIP-215857X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. DIP original experiment: DIP-215858X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. A protein kinase network regulates the function of aminophospholipid flippases. Proceedings of the National Academy of Sciences of the United States of America 2010 imex curation Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Roelants FM., Baltz AG., Trott AE., Fereres S., Thorner J. DIP original experiment: DIP-215859X Proceedings of the National Academy of Sciences of the United States of America 2010 IMEx A protein kinase network regulates the function of aminophospholipid flippases. atc5_yeast Phospholipid-transporting ATPase DNF1 DNF1 YER166W SYGP-ORF7 Flippase DNF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGTFHGDGHAPMSPFEDTFQFEDNSSNEDTHIAPTHFDDGATSNKYSRPQVSFNDETPKNKREDAEEFTFNDDTEYDNHSFQPTPKLNNGSGTFDDVELDNDSGEPHTNYDGMKRFRMGTKRNKKGNPIMGRSKTLKWARKNIPNPFEDFTKDDIDPGAINRAQELRTVYYNMPLPKDMIDEEGNPIMQYPRNKIRTTKYTPLTFLPKNILFQFHNFANVYFLVLIILGAFQIFGVTNPGLSAVPLVVIVIITAIKDAIEDSRRTVLDLEVNNTKTHILEGVENENVSTDNISLWRRFKKANSRLLFKFIQYCKEHLTEEGKKKRMQRKRHELRVQKTVGTSGPRSSLDSIDSYRVSADYGRPSLDYDNLEQGAGEANIVDRSLPPRTDCKFAKNYWKGVKVGDIVRIHNNDEIPADIILLSTSDTDGACYVETKNLDGETNLKVRQSLKCTNTIRTSKDIARTKFWIESEGPHSNLYTYQGNMKWRNLADGEIRNEPITINNVLLRGCTLRNTKWAMGVVMFTGGDTKIMLNSGITPTKKSRISRELNFSVVINFVLLFILCFVSGIANGVYYDKKGRSRFSYEFGTIAGSAATNGFVSFWVAVILYQSLVPISLYISVEIIKTAQAAFIYGDVLLYNAKLDYPCTPKSWNISDDLGQVEYIFSDKTGTLTQNVMEFKKCTINGVSYGRAYTEALAGLRKRQGIDVETEGRREKAEIAKDRDTMIDELRALSGNSQFYPEEVTFVSKEFVRDLKGASGEVQQRCCEHFMLALALCHSVLVEANPDNPKKLDLKAQSPDEAALVATARDVGFSFVGKTKKGLIIEMQGIQKEFEILNILEFNSSRKRMSCIVKIPGLNPGDEPRALLICKGADSIIYSRLSRQSGSNSEAILEKTALHLEQYATEGLRTLCIAQRELSWSEYEKWNEKYDIAAASLANREDELEVVADSIERELILLGGTAIEDRLQDGVPDCIELLAEAGIKLWVLTGDKVETAINIGFSCNLLNNEMELLVIKTTGDDVKEFGSEPSEIVDALLSKYLKEYFNLTGSEEEIFEAKKDHEFPKGNYAIVIDGDALKLALYGEDIRRKFLLLCKNCRAVLCCRVSPSQKAAVVKLVKDSLDVMTLAIGDGSNDVAMIQSADVGIGIAGEEGRQAVMCSDYAIGQFRYLARLVLVHGRWSYKRLAEMIPEFFYKNMIFALALFWYGIYNDFDGSYLYEYTYMMFYNLAFTSLPVIFLGILDQDVNDTISLVVPQLYRVGILRKEWNQRKFLWYMLDGLYQSIICFFFPYLVYHKNMIVTSNGLGLDHRYFVGVYVTTIAVISCNTYVLLHQYRWDWFSGLFIALSCLVVFAWTGIWSSAIASREFFKAAARIYGAPSFWAVFFVAVLFCLLPRFTYDSFQKFFYPTDVEIVREMWQHGHFDHYPPGYDPTDPNRPKVTKAGQHGEKIIEGIALSDNLGGSNYSRDSVVTEEIPMTFMHGEDGSPSGYQKQETWMTSPKETQDLLQSPQFQQAQTFGRGPSTNVRSSLDRTREQMIATNQLDNRYSVERARTSLDLPGVTNAASLIGTQQNN 3CC3FBA8ADDE8960 3CC3FBA8ADDE8960 fpk1_yeast Flippase kinase 1 N3449 YNR047W FPK1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAGHHHEHEQERDHEQEHEHDSLQRPTTGSERTRSISFSKLLTRSWKRNASSSNNMSVSSVNLYSDPENSRESDHNNSGSEGQSSRFSKLKSMFQSGNSSKNASAHNSSQSSLEGDSASSSSKLRYVKPMTSVANASPASPPLSPTIPETDVLQTPKMVHIDQHEHEREHSNCGSPIMLSSSSFSPTVARTGTGRRRSPSTPIMPSQNSNNSSSTSAIRPNNYRHHSGSQGFSSNNPFRERAGTVRSSNPYFAYQGLPTHAMSSHDLDEGFQPYANGSGIHFLSTPTSKTNSLTNTKNLSNLSLNEIKENEEVQEFNNEDFFFHDIPKDLSLKDTLNGSPSRGSSKSPTITQTFPSIIVGFDNEYEEDNNNDKHDEKEEQQTTTDNKTRNLSPTKQNGKATHPRIKIPLRRAASEPNGLQLASATSPTSSSARKTSGSSNINDKIPGQSVPPPNSFFPQEPSPKISDFPEPRRSRRLRTKSFSNKFQDIMVGPQSFEKIRLLGQGDVGKVFLVREKKTNRVYALKVLSKDEMIKRNKIKRVLTEQEILATSNHPFIVTLYHSFQSEDYLYLCMEYCMGGEFFRALQTRKTKCICEDDARFYASEVTAALEYLHLLGFIYRDLKPENILLHQSGHIMLSDFDLSIQAKDSKVPVVKGSAQSTLVDTKICSDGFRTNSFVGTEEYIAPEVIRGNGHTAAVDWWTLGILIYEMLFGFTPFKGDNTNETFTNILKNEVSFPNNNEISRTCKDLIKKLLTKNESKRLGCKMGAADVKKHPFFKKVQWSLLRNQEPPLIPVLSEDGYDFAKLSSNKKRQTSQDSHKHLDEQEKNMFEERVEYDDEVSEDDPFHDFNSMSLMEQDNNSMIYGNTNSYGKIAYTPNSNRSRSNSHRTFFKR 26AF74EE956F80DB 26AF74EE956F80DB ypk1_yeast Serine/threonine-protein kinase YPK1 YPK1 Yeast protein kinase 1 Sphingosine-like immunosuppressant resistant protein 2 YKL126W SLI2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MYSWKSKFKFGKSKEEKEAKHSGFFHSSKKEEQQNNQATAGEHDASITRSSLDRKGTINPSNSSVVPVRVSYDASSSTSTVRDSNGGNSENTNSSQNLDETANIGSTGTPNDATSSSGMMTIKVYNGDDFILPFPITSSEQILNKLLASGVPPPHKEISKEVDALIAQLSRVQIKNQGPADEDLISSESAAKFIPSTIMLPGSSTLNPLLYFTIEFDNTVATIEAEYGTIAKPGFNKISTFDVTRKLPYLKIDVFARIPSILLPSKTWQQEMGLQDEKLQTIFDKINSNQDIHLDSFHLPINLSFDSAASIRLYNHHWITLDNGLGKINISIDYKPSRNKPLSIDDFDLLKVIGKGSFGKVMQVRKKDTQKVYALKAIRKSYIVSKSEVTHTLAERTVLARVDCPFIVPLKFSFQSPEKLYFVLAFINGGELFYHLQKEGRFDLSRARFYTAELLCALDNLHKLDVVYRDLKPENILLDYQGHIALCDFGLCKLNMKDDDKTDTFCGTPEYLAPELLLGLGYTKAVDWWTLGVLLYEMLTGLPPYYDEDVPKMYKKILQEPLVFPDGFDRDAKDLLIGLLSRDPTRRLGYNGADEIRNHPFFSQLSWKRLLMKGYIPPYKPAVSNSMDTSNFDEEFTREKPIDSVVDEYLSESVQKQFGGWTYVGNEQLGSSMVQGRSIR 00112BBB849CD2B5 00112BBB849CD2B5 fpk1-ypk1-1 2 7 enzyme enzyme unspecified role unspecified role P53739:p.Asp621Ala D621A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D621A'. Wed May 02 19:03:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp621ala' Tue Jun 30 18:29:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp621Ala' ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-347 Region 1-347 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation dnf1-fpk1-1 5 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1404-1571 Region 1404-1571 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P32660:p.[Ser1526Ala;Ser1552Ala] S1526A/S1552A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S1526A/S1552A'. Wed May 02 18:28:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser1526ala,ser1552ala' Tue Jun 30 18:46:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser1526Ala;Ser1552Ala]' ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation fpk1-1 3 7 putative self putative self unspecified role unspecified role P53739:p.Asp621Ala D621A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D621A'. Wed May 02 17:08:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp621ala' Tue Jun 30 18:35:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp621Ala' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation true dnf1-fpk1-2 4 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1404-1571 Region 1404-1571 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 7 enzyme enzyme unspecified role unspecified role P53739:p.Asp621Ala D621A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A This feature has been corrected as a result of our quality control procedures. The original label was 'D621A'. Wed May 02 15:18:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp621ala' Tue Jun 30 17:50:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp621Ala' ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation fpk1-ypk1-2 1 7 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 8 enzyme target enzyme target substrate substrate unspecified role unspecified role P12688:p.[Ser51Ala;Ser71Ala] S51A/S71A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S51A/S71A'. Wed May 02 14:44:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser51ala,ser71ala' Tue Jun 30 17:47:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser51Ala;Ser71Ala]' Region 1-100 Region 1-100 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation