DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Signal peptides are allosteric activators of the protein translocase. Signal peptides are allosteric activators of the protein translocase. Nature 2009 imex curation Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. DIP original experiment: DIP-219608X Nature 2009 IMEx Signal peptides are allosteric activators of the protein translocase. Signal peptides are allosteric activators of the protein translocase. Signal peptides are allosteric activators of the protein translocase. Nature 2009 imex curation Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. DIP original experiment: DIP-219607X Nature 2009 IMEx Signal peptides are allosteric activators of the protein translocase. Signal peptides are allosteric activators of the protein translocase. Signal peptides are allosteric activators of the protein translocase. Nature 2009 imex curation Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Gouridis G., Karamanou S., Gelis I., Kalodimos CG., Economou A. DIP original experiment: DIP-219609X Nature 2009 IMEx Signal peptides are allosteric activators of the protein translocase. secy_ecoli Protein translocase subunit SecY secY prlA b3300 JW3262 protein protein ecoli Escherichia coli (strain K12) MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGIDAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTLAEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTAVVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFLVLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIPAIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTALVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMRDAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR DIP protein P0AGA2 original sequence version: 78 DIP protein P0AGA2 original sequence version: 60 DIP protein P0AGA2 original sequence version: 41 DIP protein P0AGA2 original sequence version: 56 DIP protein P0AGA2 original sequence version: 49 711CA63CD8809763 secg_ecoli Protein-export membrane protein SecG P12 Preprotein translocase band 1 subunit JW3142 b3175 secG protein protein ecoli Escherichia coli (strain K12) MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFGSSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPAKPTSDIPN DIP protein P0AG99 original sequence version: 77 DIP protein P0AG99 original sequence version: 39 DIP protein P0AG99 original sequence version: 54 DIP protein P0AG99 original sequence version: 46 8DC7663F94D32F2F seca_ecoli Protein translocase subunit SecA secA azi pea prlD b0098 JW0096 protein protein ecoli Escherichia coli (strain K12) MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELKGKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEMRTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPGMPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDEARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTERGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVIIVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTGTADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQPVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAGRGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRRIDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVTKAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIREDVFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERILAQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKDPKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQLSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ DIP protein P10408 original sequence version: 150 DIP protein P10408 original sequence version: 130 DIP protein P10408 original sequence version: 105 DIP protein P10408 original sequence version: 107 DIP protein P10408 original sequence version: 125 DIP protein P10408 original sequence version: 140 DIP protein P10408 original sequence version: null 683D66DD4305DBC2 ppb_ecoli Alkaline phosphatase phoA b0383 JW0374 protein protein ecoli Escherichia coli (strain K12) MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGARRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPLTGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKAAGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLLNARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKPLLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSKNEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADHAHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAANVVGLTDQTDLFYTMKAALGLK DIP protein P00634 original sequence version: 122 DIP protein P00634 original sequence version: 123 DIP protein P00634 original sequence version: null 8A8DE1F29D9D9253 sece_ecoli Protein translocase subunit SecE JW3944 b3981 secE prlG protein protein ecoli Escherichia coli (strain K12) MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLRALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTAVMSLILWGLDGILVRLVSFITGLRF DIP protein P0AG96 original sequence version: 42 DIP protein P0AG96 original sequence version: 58 DIP protein P0AG96 original sequence version: 50 94D37280522875CE phoa-seca-1 3 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Mature protein Region 23-471 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction secy-sece-3 2 4 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role prey prey ecolx Escherichia coli association association phoa-seca-2 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Signal peptide Region 1-21 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction