DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. DIP original experiment: DIP-134773X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. Only protein-protein interactions in vitro In vitro electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. DIP original experiment: DIP-134771X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. Only protein-protein interactions ecolx Escherichia coli pull down pull down predetermined predetermined participant predetermined Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. DIP original experiment: DIP-134772X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hudson BP., Quispe J., Lara-González S., Kim Y., Berman HM., Arnold E., Ebright RH., Lawson CL. DIP original experiment: DIP-134774X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Three-dimensional EM structure of an intact activator-dependent transcription initiation complex. siga_theaq RNA polymerase sigma factor SigA sigA protein protein theaq Thermus aquaticus MKKSKSKKKAAKAQEVEVKEPVKEPEPLPELEAAEDLQDLPEPDPELLASEPELEDLADPLDLEGPLEADLLPEEGLLEEEEEELSLPKVSTSDPVRQYLHEIGQVPLLTLEEEIDLARKVEEGMEAIKKLSEATGLDQELIREVVRAKILGTARIQKIPGLKEKPDPKTVEEVDGKLKSLPKELKRYLHIAREGEAARQHLIEANLRLVVSIAKKYTGRGLSFLDLIQEGNQGLIRAVEKFEYKRRFKFSTYATWWIRQAINRAIADQARTIRIPVHMVETINKLSRTARQLQQELGREPSYEEIAEAMGPGWDAKRVEETLKIAQEPVSLETPIGDEKDSFYGDFIPDENLPSPVEAAAQSLLSEELEKALSKLSEREAMVLKLRKGLIDGREHTLEEVGAYFGVTRERIRQIENKALRKLKYHESRTRKLRDFLE DIP protein Q9EZJ8 original sequence version: 45 5D8ACF5713DE55AD crp_ecoli cAMP-activated global transcriptional regulator CRP Catabolite activator protein crp cap csm b3357 cAMP regulatory protein JW5702 cAMP receptor protein Catabolite gene activator protein protein ecoli Escherichia coli (strain K12) MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTHPDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR DCBC24FA46C61B3D DCBC24FA46C61B3D rpod_ecoli RNA polymerase sigma factor RpoD rpoD alt b3067 Sigma-70 JW3039 protein protein ecoli Escherichia coli (strain K12) MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSDLITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAREKFAELRAQYVVTRDTIKAKGRSHATAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVMMDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVSEEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGIDMNTDYTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD DIP protein P00579 original sequence version: 148 DIP protein P00579 original sequence version: 114 DIP protein P00579 original sequence version: 162 DIP protein P00579 original sequence version: 136 DIP protein P00579 original sequence version: null CA4F0E30DEC1703D rpoc_ecoli DNA-directed RNA polymerase subunit beta' rpoC tabB b3988 JW3951 Transcriptase subunit beta' RNA polymerase subunit beta' protein protein ecoli Escherichia coli (strain K12) MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETINYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERMGHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQILTEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLTKRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRNNRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGKQGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLATTIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLHPLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVLGLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAKTSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQIMYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKVIDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQLAGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTRRLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADILVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEAIGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGKLVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITEVSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDVLIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFEARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERVERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLRKATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFISAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEAPAAPQVTAEDASASLAELLNAGLGGSDNE DIP protein P0A8T7 original sequence version: 50 DIP protein P0A8T7 original sequence version: 55 DIP protein P0A8T7 original sequence version: 105 DIP protein P0A8T7 original sequence version: 89 A6878C61D15F4961 rpoa_ecoli DNA-directed RNA polymerase subunit alpha rpoA pez phs sez b3295 Transcriptase subunit alpha RNA polymerase subunit alpha JW3257 protein protein ecoli Escherichia coli (strain K12) MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALRRILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTLNKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIHSEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIRRAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIHYIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE DIP protein P0A7Z4 original sequence version: 55 DIP protein P0A7Z4 original sequence version: 60 DIP protein P0A7Z4 original sequence version: 69 DIP protein P0A7Z4 original sequence version: 107 DIP protein P0A7Z4 original sequence version: 93 12A14B75A3CAEA19 rpoz_ecoli DNA-directed RNA polymerase subunit omega rpoZ b3649 Transcriptase subunit omega RNA polymerase omega subunit JW3624 protein protein ecoli Escherichia coli (strain K12) MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEENDKTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR DIP protein P0A800 original sequence version: 44 DIP protein P0A800 original sequence version: 89 DIP protein P0A800 original sequence version: 76 4DE7E81EF670D887 rpob_ecoli DNA-directed RNA polymerase subunit beta rpoB groN nitB rif ron stl stv tabD b3987 Transcriptase subunit beta RNA polymerase subunit beta JW3950 protein protein ecoli Escherichia coli (strain K12) MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPEGQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRLVIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSDKGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTEQILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQLEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIETLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSEDRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGNRRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGSSQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLDEEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMGANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDEMYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQNMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPNVGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVKDSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLFSRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLEAKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYDENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQRAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDLPTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMPESFNVLLKEIRSLGINIELEDE DIP protein P0A8V2 original sequence version: 93 DIP protein P0A8V2 original sequence version: 58 DIP protein P0A8V2 original sequence version: 82 F9E95344C54AB118 crp-rpoa-1 2 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association rpoa-siga-1 4 9 unspecified role unspecified role unspecified role unspecified role C-terminal domain Region 246-329 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 366-438 Region 366-438 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction rpoa-rpob-6 1 9 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association rpoc-rpoa-1 3 9 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli 10 unspecified role unspecified role prey prey ecolx Escherichia coli 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role prey prey ecolx Escherichia coli association association