IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ KIR2DS4 is a product of gene conversion with KIR3DL2 that introduced specificity for HLA-A*11 while diminishing avidity for HLA-C. KIR2DS4 is a product of gene conversion with KIR3DL2 that introduced specificity for HLA-A*11 while diminishing avidity for HLA-C. The Journal of experimental medicine 2009 imex curation Graef T., Moesta AK., Norman PJ., Abi-Rached L., Vago L., Older Aguilar AM., Gleimer M., Hammond JA., Guethlein LA., Bushnell DA., Robinson PJ., Parham P. Pablo suggested using a Variant Database as XRef for the HLA variants. Immuno Polymorphism Database (IPD) (http://www.ebi.ac.uk/ipd/mhc/) has possible identifiers/XRef for MHC alleles- results in "allele reports";these could be added as XRefs if this was possible (not currently). The variant SNPs do not necessarily map to a unique location in Ensembl so not possible to give an Ensembl link. We have no DbSNP link currently, so entry given as free text in a Comment along with Allele designation. (I have entered Allele names in the from "C0201"-which is reasonably clear, as UniProt/this publication/IPD/other sources all differ w.r.t. name format-cannot use current preffered format as this publication is using pre 2010 nomenclature. The human KIRs used to make the fusion proteins are stated to be of alleles:-KIR2DL1*003, KIR2DL2*001, and KIR2DL3*001. Uniprot gives the sequence for KIR2DL1*001. Differences in the KIR2DL1*003 variant as compared to the KIR2DL1*001 sequence are:-P37R and P135L. (https://www.ebi.ac.uk/cgi-bin/ipd/kir/get_allele.cgi?2DL1*00301) peropa@stanford.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom antibody detection identification by antibody antibody detection Graef T., Moesta AK., Norman PJ., Abi-Rached L., Vago L., Older Aguilar AM., Gleimer M., Hammond JA., Guethlein LA., Bushnell DA., Robinson PJ., Parham P. The common variant- KIR2DS4*003 allele has a 22nt deletion in exon 5 resulting in a frameshift and loss of the C-terminal membrane binding domain. The resulting protein has a distinct C-teminus and is likely secreted. KIR2DS4*003-Fc did not bind to any HLA Class one molecules tested here. Pablo suggested using a Variant Database as XRef for the HLA variants. Immuno Polymorphism Database (IPD) (http://www.ebi.ac.uk/ipd/mhc/) has possible identifiers/XRef for MHC alleles- results in "allele reports";these could be added as XRefs if this was possible (not currently). The variant SNPs do not necessarily map to a unique location in Ensembl so not possible to give an Ensembl link. We have no DbSNP link currently, so entry given as free text in a Comment along with Allele designation. (I have entered Allele names in the from "C0201"-which is reasonably clear, as UniProt/this publication/IPD/other sources all differ w.r.t. name format-cannot use current preffered format as this publication is using pre 2010 nomenclature. LABScreen single-Ag bead sets (One Lambda) were used: Various HLA Class 1 molecules were bound to beads and incubated with KIR2DS4-Fc protein. Binding was detected by fluorescence emitted by a Phycoerythrin conjugated anti-Fc antibody. The human KIRs used to make the fusion proteins are stated to be of alleles:-KIR2DL1*003, KIR2DL2*001, and KIR2DL3*001. Uniprot gives the sequence for KIR2DL1*001. Differences in the KIR2DL1*003 variant as compared to the KIR2DL1*001 sequence are:-P37R and P135L. (https://www.ebi.ac.uk/cgi-bin/ipd/kir/get_allele.cgi?2DL1*00301) peropa@stanford.edu Accepted 2017-MAR-31 AT 08:47 BST AT 08:47 BST by ORCHARD The Journal of experimental medicine 2009 imex curation I have added "Fig1" to everything here. Also added the P37R and P135L for the KIR2DL1*003 variant (P43626). Data-Proc. statement copied over from Moesta with slight alteration. C1701 and 1502 alleles ARE in the Moesta-2008 paper, and results added for IntAct. KIR2DS4 is a product of gene conversion with KIR3DL2 that introduced specificity for HLA-A*11 while diminishing avidity for HLA-C. KIR2DS4 is a product of gene conversion with KIR3DL2 that introduced specificity for HLA-A*11 while diminishing avidity for HLA-C. The Journal of experimental medicine 2009 imex curation Graef T., Moesta AK., Norman PJ., Abi-Rached L., Vago L., Older Aguilar AM., Gleimer M., Hammond JA., Guethlein LA., Bushnell DA., Robinson PJ., Parham P. Pablo suggested using a Variant Database as XRef for the HLA variants. Immuno Polymorphism Database (IPD) (http://www.ebi.ac.uk/ipd/mhc/) has possible identifiers/XRef for MHC alleles- results in "allele reports";these could be added as XRefs if this was possible (not currently). The variant SNPs do not necessarily map to a unique location in Ensembl so not possible to give an Ensembl link. We have no DbSNP link currently, so entry given as free text in a Comment along with Allele designation. (I have entered Allele names in the from "C0201"-which is reasonably clear, as UniProt/this publication/IPD/other sources all differ w.r.t. name format-cannot use current preffered format as this publication is using pre 2010 nomenclature. The human KIRs used to make the fusion proteins are stated to be of alleles:-KIR2DL1*003, KIR2DL2*001, and KIR2DL3*001. Uniprot gives the sequence for KIR2DL1*001. Differences in the KIR2DL1*003 variant as compared to the KIR2DL1*001 sequence are:-P37R and P135L. (https://www.ebi.ac.uk/cgi-bin/ipd/kir/get_allele.cgi?2DL1*00301) peropa@stanford.edu 18-Apr-2017: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom antibody detection identification by antibody antibody detection Graef T., Moesta AK., Norman PJ., Abi-Rached L., Vago L., Older Aguilar AM., Gleimer M., Hammond JA., Guethlein LA., Bushnell DA., Robinson PJ., Parham P. Pablo suggested using a Variant Database as XRef for the HLA variants. Immuno Polymorphism Database (IPD) (http://www.ebi.ac.uk/ipd/mhc/) has possible identifiers/XRef for MHC alleles- results in "allele reports";these could be added as XRefs if this was possible (not currently). The variant SNPs do not necessarily map to a unique location in Ensembl so not possible to give an Ensembl link. We have no DbSNP link currently, so entry given as free text in a Comment along with Allele designation. (I have entered Allele names in the from "C0201"-which is reasonably clear, as UniProt/this publication/IPD/other sources all differ w.r.t. name format-cannot use current preffered format as this publication is using pre 2010 nomenclature. LABScreen single-Ag bead sets (One Lambda) were used: Various HLA Class 1 molecules were bound to beads and incubated with KIR2DL1/3-Fc protein. Binding was detected by fluorescence emitted by a Phycoerythrin conjugated anti-Fc antibody. The human KIRs used to make the fusion proteins are stated to be of alleles:-KIR2DL1*003, KIR2DL2*001, and KIR2DL3*001. Uniprot gives the sequence for KIR2DL1*001. Differences in the KIR2DL1*003 variant as compared to the KIR2DL1*001 sequence are:-P37R and P135L. (https://www.ebi.ac.uk/cgi-bin/ipd/kir/get_allele.cgi?2DL1*00301) peropa@stanford.edu Accepted 2017-MAR-31 AT 08:47 BST AT 08:47 BST by ORCHARD The Journal of experimental medicine 2009 imex curation I have added "Fig1" to everything here. Also added the P37R and P135L for the KIR2DL1*003 variant (P43626). Data-Proc. statement copied over from Moesta with slight alteration. C1701 and 1502 alleles ARE in the Moesta-2008 paper, and results added for IntAct. hlac_human HLA class I histocompatibility antigen, C alpha chain HLA-Cw Human leukocyte antigen C HLAC HLA-C protein protein human Homo sapiens Human MRVMAPRALLLLLSGGLALTETWACSHSMRYFDTAVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQADRVSLRNLRGYYNQSEDGSHTLQRMSGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKLEAARAAEQLRAYLEGTCVEWLRRYLENGKETLQRAEPPKTHVTHHPLSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGQEQRYTCHMQHEGLQEPLTLSWEPSSQPTIPIMGIVAGLAVLVVLAVLGAVVTAMMCRRKSSGGKGGSCSQAACSNSAQGSDESLITCKA DIP protein P30504 original sequence version: 126 59C23D95FD1D0BC8 1c02_human HLA class I histocompatibility antigen, Cw-2 alpha chain HLA-C HLAC MHC class I antigen Cw*2 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMRYFYTAVSRPSRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRGRWVEQEGPEYWDRETQKYNRQAQTDRVNLRKLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYDQSAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEEWRAYLEGECVEWLRRYLENGKEKLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPTEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). AD8025DEB7DA8CE6 ki2s4_human Killer cell immunoglobulin-like receptor 2DS4 KIR2DS4 CD158I KKA3 NKAT8 CD158 antigen-like family member I MHC class I NK cell receptor Natural killer-associated transcript 8 P58 natural killer cell receptor clones CL-39/CL-17 protein protein human Homo sapiens Human MSLMVIIMACVGFFLLQGAWPQEGVHRKPSFLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNNTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDPLDMVIIGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAVRSINGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSDKKNAAVMDQEPAGNRTVNSEDSDEQDHQEVSYA CEFDAFF3FD679A3F ki2l1_human Killer cell immunoglobulin-like receptor 2DL1 KIR2DL1 CD158 antigen-like family member A CD158A NKAT1 MHC class I NK cell receptor Natural killer-associated transcript 1 p58 natural killer cell receptor clones CL-42/47.11 p58.1 MHC class-I-specific NK receptor protein protein human Homo sapiens Human MSLLVVSMACVGFFLLQGAWPHEGVHRKPSLLAHPGPLVKSEETVILQCWSDVMFEHFLLHREGMFNDTLRLIGEHHDGVSKANFSISRMTQDLAGTYRCYGSVTHSPYQVSAPSDPLDIVIIGLYEKPSLSAQPGPTVLAGENVTLSCSSRSSYDMYHLSREGEAHERRLPAGPKVNGTFQADFPLGPATHGGTYRCFGSFHDSPYEWSKSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHILIGTSVVIILFILLFFLLHRWCSNKKNAAVMDQESAGNRTANSEDSDEQDPQEVTYTQLNHCVFTQRKITRPSQRPKTPPTDIIVYTELPNAESRSKVVSCP mint 3DFBF2D6FDCE1D1E 1c01_human HLA class I histocompatibility antigen, Cw-1 alpha chain HLA-C HLAC MHC class I antigen Cw*1 protein protein human Homo sapiens Human MRVMEPRTLILLLSGALALTETWACSHSMKYFFTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYNRQAQTDRVSLRNLRGYYNQSEAGSHTLQWMCGCDLGPDGRLLRGYDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEERRAYLEGTCVEWLRRYLENGKESLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQWDGEDQTQDTELVETRPAGDGTFQKWAAVMVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIASKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). 285CCFCB92788744 f2vzg2_pantr KIR2DS4 protein protein pantr Pan troglodytes (Chimpanzee) Chimpanzee MSLMVVSMACVGFFLLQGAWPHEGVQIKPSLLALPGHLVKSEETVILQCWSDVMFEHFLLHREGKFNDTLHLIGEHHDGVSKANFSIGPMMPVLAGTYRCYGSVPHSPYQLSAPSDHLDIVITGLYEKPSLSAQPGPTVQAGENVTLSCSSRSSYDMYHLSREGEVHERRLPAVRSIKGTFQADFPLGPATHGGTYRCFGSFRDAPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSKTGNPRHLHVLIGTSVVKIPFTILLFFLLHRWCSNKKKCCRNGPRACREQNSEPGGF 7AFA8D79329F51A0 ki2l3_human Killer cell immunoglobulin-like receptor 2DL3 KIR2DL3 KIRCL23 NKAT2 CD158B2 MHC class I NK cell receptor Natural killer-associated transcript 2 NKAT2a NKAT2b p58 natural killer cell receptor clone CL-6 p58.2 MHC class-I-specific NK receptor Killer inhibitory receptor cl 2-3 KIR-023GB CD158 antigen-like family member B2 protein protein human Homo sapiens Human MSLMVVSMVCVGFFLLQGAWPHEGVHRKPSLLAHPGPLVKSEETVILQCWSDVRFQHFLLHREGKFKDTLHLIGEHHDGVSKANFSIGPMMQDLAGTYRCYGSVTHSPYQLSAPSDPLDIVITGLYEKPSLSAQPGPTVLAGESVTLSCSSRSSYDMYHLSREGEAHERRFSAGPKVNGTFQADFPLGPATHGGTYRCFGSFRDSPYEWSNSSDPLLVSVTGNPSNSWPSPTEPSSETGNPRHLHVLIGTSVVIILFILLLFFLLHRWCCNKKNAVVMDQEPAGNRTVNREDSDEQDPQEVTYAQLNHCVFTQRKITRPSQRPKTPPTDIIVYTELPNAEP mint Receptor on natural killer (NK) cells for HLA-C alleles (HLA-Cw1, HLA-Cw3 and HLA-Cw7). Inhibits the activity of NK cells thus preventing cell lysis C21EAFF80EA9AAB2 q5s3g3_human HLA-A HLAA MHC class I antigen A*11 protein protein human Homo sapiens Human MAVMAPRTLLLLLSGALALTQTWAGSHSMRYFYTSVSRPGRGEPRFIAVGYVDDTQFVRFDSDAASQRMEPRAPWIEQEGPEYWDQETRNVKAQSQTDRVDLGTLRGYYNQSEDGSHTIQIMYGCDVGPDGRFLRGYRQDAYDGKDYIALNEDLRSWTAADMAAQITKRKWEAAHAAEQQRAYLEGRCVEWLRRYLENGKETLQRTDPPKTHMTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWELSSQPTIPIVGIIAGLVLLGAVITGAVVAAVMWRRKSSDRKGGSYTQAASSDSAQGSDVSLTACKV FE449CE2D4BF6CC5 1c03_human HLA class I histocompatibility antigen, Cw-3 alpha chain HLA-C HLAC MHC class I antigen Cw*3 protein protein human Homo sapiens Human MRVMAPRTLILLLSGALALTETWAGSHSMRYFYTAVSRPGRGEPHFIAVGYVDDTQFVRFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQTDRVSLRNLRGYYNQSEAGSHIIQRMYGCDVGPDGRLLRGYDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQLRAYLEGLCVEWLRRYLKNGKETLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQWDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWEPSSQPTIPIVGIVAGLAVLAVLAVLGAVVAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIACKA The protein could not be merged with EBI-1051396 because of some conflicts with the protein sequence (features which cannot be shifted). 47E55680EA1F6D97 hlab_human HLA class I histocompatibility antigen, B alpha chain Human leukocyte antigen B HLA-B HLAB protein protein human Homo sapiens Human MLVMAPRTVLLLLSAALALTETWAGSHSMRYFYTSVSRPGRGEPRFISVGYVDDTQFVRFDSDAASPREEPRAPWIEQEGPEYWDRNTQIYKAQAQTDRESLRNLRGYYNQSEAGSHTLQSMYGCDVGPDGRLLRGHDQYAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAAREAEQRRAYLEGECVEWLRRYLENGKDKLERADPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDRTFQKWAAVVVPSGEEQRYTCHVQHEGLPKPLTLRWEPSSQSTVPIVGIVAGLAVLAVVVIGAVVAAVMCRRKSSGGKGGSYSQAACSDSAQGSDVSLTA 5E5A7BDE031403D6 1c08_human HLA class I histocompatibility antigen, Cw-8 alpha chain HLA-C HLAC MHC class I antigen Cw*8 protein protein human Homo sapiens Human MRVMAPRTLILLLSGALALTETWACSHSMRYFYTAVSRPGRGEPRFIAVGYVDDTQFVQFDSDAASPRGEPRAPWVEQEGPEYWDRETQKYKRQAQTDRVSLRNLRGYYNQSEAGSHTLQRMYGCDLGPDGRLLRGYNQFAYDGKDYIALNEDLRSWTAADTAAQITQRKWEAARTAEQLRAYLEGTCVEWLRRYLENGKKTLQRAEHPKTHVTHHPVSDHEATLRCWALGFYPAEITLTWQRDGEDQTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPLTLRWGPSSQPTIPIVGIVAGLAVLAVLAVLGAVMAVVMCRRKSSGGKGGSCSQAASSNSAQGSDESLIACKA 2A84D41389A0486A hla_c16-kir2ds4-1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:26:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 19:24:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 16:57:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 19:53:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Ala205Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:58:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 17:56:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' P43632:p.Pro92_Val93delinsGlnAsp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 19:12:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 19:31:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1,5 C1601 allele hla_c01-kir2ds4-1 1 7 unspecified role unspecified role bait bait asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q ser202thr variant variant certain certain sequence position certain certain certain sequence position certain S T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:21:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 18:11:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Ala205Ser mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 20:25:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 16:38:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 17:07:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 15:41:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Pro92_Val93delinsGlnAsp mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 14:51:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 18:42:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-2,5 C0102 allele;weak binding hla_c14-kir2ds4-2 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:23:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 20:03:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Pro92_Val93delinsGlnAsp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 17:05:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 18:10:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 14:54:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 19:03:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Ala205Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A S Wed May 02 17:59:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 16:40:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-3,5 C1402 allele;weak binding hla_c05-kir2ds4-1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:17:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 19:56:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Ala205Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:45:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 17:54:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' P43632:p.Pro92_Val93delinsGlnAsp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 16:11:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 17:34:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 15:18:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 16:12:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-4,5 C0501 allele hla0202_c-kir2ds4-1 1 4 unspecified role unspecified role bait bait glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain K T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 17:49:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 16:39:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Lys65Met mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:01:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 17:45:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' P43632:p.Pro92_Val93delinsGlnAsp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 19:20:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 19:50:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A S Wed May 02 18:29:21 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 17:48:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-5,5 C0202 allele hla_c04-kir2ds4-1 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain K M Wed May 02 17:32:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 15:45:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 17:14:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 19:32:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Ala205Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A S Wed May 02 15:51:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 16:19:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Pro92_Val93delinsGlnAsp mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 18:50:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 16:13:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-6,5 C04:01 allele;very low binding hla_a11-kir2ds4-1 1 10 unspecified role unspecified role bait bait Q5S3G3:p.Glu43Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 15:55:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu43lys' Mon Mar 01 11:51:22 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu43Lys' 5 unspecified role unspecified role prey prey region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain P43632:p.Lys65Met mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K M Wed May 02 20:20:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 17:51:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Ile123Thr mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain I T Wed May 02 16:33:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile123thr' Tue Jun 30 19:32:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile123Thr' P43632:p.Pro92_Val93delinsGlnAsp mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 16:48:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 15:48:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain A S Wed May 02 16:21:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 16:26:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 1-7,5 A1102 (E43K) and A1101 alleles hla_a-kir2ds4-1 1 10 unspecified role unspecified role bait bait Q5S3G3:p.Glu43Lys mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:13:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu43lys' Mon Mar 01 11:09:04 GMT 2021 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu43Lys' 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-1 A1102 (E43K) and A1101 alleles;both alleles bind. hla_c-kir2ds4-1 1 7 unspecified role unspecified role bait bait asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q ser202thr variant variant certain certain sequence position certain certain certain sequence position certain S T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-2 C0102 allele;weak binding hla_c-kir2ds4-2 1 11 unspecified role unspecified role bait bait tyr140ser variant variant certain certain sequence position certain certain certain sequence position certain Y S ile119leu variant variant certain certain sequence position certain certain certain sequence position certain I L 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-3 C0302 allele;weak binding hla_c-kir2ds4-3 1 13 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-4 C0801 allele;weak binding hla_c-kir2ds4-4 1 3 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-5 C1601 allele hla_c-kir2ds4-5 1 3 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 3-6 C1502 allele;very weak binding hla_c-kir2ds4-6 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey P43632:p.Lys65Met mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain K M Wed May 02 15:51:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 18:52:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' P43632:p.Pro92_Val93delinsGlnAsp mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 16:31:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 18:18:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:31:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 17:27:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5 C1701 allele; low binding hla_c-kir2ds4-7 1 3 unspecified role unspecified role bait bait val272met variant variant certain certain sequence position certain certain certain sequence position certain V M 5 unspecified role unspecified role prey prey P43632:p.Lys65Met mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain K M Wed May 02 16:46:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys65met' Tue Jun 30 20:07:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys65Met' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5i C1802 allele; low binding hla_b4601-kir2ds4-1 1 12 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Pro92_Val93delinsGlnAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 16:58:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 16:39:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:35:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 20:09:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5ii B4601 allele hla_b7301-kir2ds4-1 1 12 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey P43632:p.Pro92_Val93delinsGlnAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 18:21:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 17:59:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 16:57:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 19:52:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5iii B7301 allele hla_c0302-kir2ds4-8 1 11 unspecified role unspecified role bait bait gly115arg variant variant certain certain sequence position certain certain certain sequence position certain G R tyr140ser,ile119leu variant variant certain certain sequence position certain certain certain sequence position certain Y S certain certain sequence position certain certain certain sequence position certain I L 5 unspecified role unspecified role prey prey P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 17:56:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 19:49:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' P43632:p.Pro92_Val93delinsGlnAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 15:25:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 20:25:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5 C0302, C0304 and C0303 alleles;all alleles showed similar binding. I119L variant:dbSNP:rs1071649; Y140S variant dbSNP:rs713032; G115R variant dbSNP:rs1131122 hla_c0702-kir2ds4-8 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43632:p.Pro92_Val93delinsGlnAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 19:46:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 16:52:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 17:32:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 16:56:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5 C0702 allele; weak interaction hla_c0801-kir2ds4-8 1 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey P43632:p.Pro92_Val93delinsGlnAsp mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain PV QD Wed May 02 17:16:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro_val92-93gln_asp' Tue Jun 30 17:29:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro92_Val93delinsGlnAsp' P43632:p.Ala205Ser mutation causing mutation causing an interaction mutation enabling interaction mutation causing mutation causing an interaction certain certain sequence position certain certain certain sequence position certain A S Wed May 02 14:42:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala205ser' Tue Jun 30 17:48:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala205Ser' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. direct interaction direct interaction Fig 5 C0801 allele hla_c-kir2ds4-8 2 6 unspecified role unspecified role prey prey P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:54:46 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 16:39:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 16:13:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:31:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 18:30:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 16:56:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 17:27:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:58:25 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 19:04:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 19:02:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait val272met variant variant certain certain sequence position certain certain certain sequence position certain V M direct interaction direct interaction Fig 1, Supp Fig 3-1 C1802 allele hla_c-kir2dl1-1 2 6 unspecified role unspecified role prey prey pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Wed May 02 16:31:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 16:14:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 16:12:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 18:10:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 20:30:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 19:10:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:48:18 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 19:39:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 18:40:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-2 C1701 allele hla_c-kir2dl1-2 2 6 unspecified role unspecified role prey prey P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:44:18 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 19:59:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 17:37:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:35:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:48:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 16:08:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 17:37:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:51:36 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 15:43:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 17:03:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-3 C1502 allele hla_c-kir2dl1-3 2 6 unspecified role unspecified role prey prey pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:42:16 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 20:02:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 18:24:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 14:32:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 15:46:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 15:40:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 17:38:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:53:44 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 17:00:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 16:09:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-4 C0602 allele hla_c-kir2dl1-4 2 6 unspecified role unspecified role prey prey pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:48:01 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 14:53:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 17:11:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:21:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 17:17:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 18:49:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 20:18:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:43:29 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 19:52:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 16:00:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-5 C0501 allele hla_c-kir2dl1-5 2 6 unspecified role unspecified role prey prey P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:41:23 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 15:36:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 19:43:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:36:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 18:26:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 19:08:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 18:41:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:54:32 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 20:02:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 19:32:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-6 C0401 allele hla_c-kir2dl1-6 2 6 unspecified role unspecified role prey prey P43626:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:57:17 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 19:38:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 16:43:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43626:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:30:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:02:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43626:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 18:31:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 20:08:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43626:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:48:59 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 16:58:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 15:40:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' pro37arg,pro135leu variant variant certain certain sequence position certain certain certain sequence position certain P R certain certain sequence position certain certain certain sequence position certain P L trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 4 unspecified role unspecified role bait bait glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A ile10leu variant variant certain certain sequence position certain certain certain sequence position certain I L gly_arg_73-74ala_pro variant variant certain certain sequence position certain certain certain sequence position certain GR AP asn92lys variant variant certain certain sequence position certain certain certain sequence position certain N K glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q lys202thr variant variant certain certain sequence position certain certain certain sequence position certain ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C direct interaction direct interaction Fig 1, Supp Fig 3-7 C0202 allele hla_c-kir2dl1-7 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:45:27 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 14:35:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 17:17:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 19:51:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 17:23:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 18:49:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 19:06:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:52:42 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 16:39:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 16:12:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-19 C0201 allele;weak interaction this allele is "HLA-C" - Top RHS - initially got missed out in numbering. hla_c-kir2dl3-1 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:57:29 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 15:19:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 17:15:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 14:48:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 17:45:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 17:12:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 18:05:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:42:41 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 19:16:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 20:02:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-8 C1601 hla_c-kir2dl3-2 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:49:32 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 18:05:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 20:01:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 17:32:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:14:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 14:33:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 18:50:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:40:55 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 17:28:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 17:59:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-9 C0801 hla_c-kir2dl3-3 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:49:05 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 19:40:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 17:39:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 18:19:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:45:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 19:50:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 16:42:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:53:53 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 16:01:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 17:07:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-10 C0702 hla_c-kir2dl3-4 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:47:58 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 18:16:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 19:31:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 15:26:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 17:44:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 20:09:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 18:06:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:53:45 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 15:19:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 19:10:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 11 unspecified role unspecified role bait bait ile119leu variant variant certain certain sequence position certain certain certain sequence position certain I L tyr140ser variant variant certain certain sequence position certain certain certain sequence position certain Y S direct interaction direct interaction Fig 1, Supp Fig 3-11 C0304 and C0302 alleles;both show same results I119L variant:dbSNP:rs1071649; Y140S variant dbSNP:rs713032; G115R variant dbSNP:rs1131122 hla_c-kir2dl3-5 2 9 unspecified role unspecified role prey prey P43628:p.Gln92_Asp93delinsProVal mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV Mon Feb 27 13:47:44 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val' Wed May 02 15:19:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val' Tue Jun 30 15:58:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92_Asp93delinsProVal' region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation increasing strength mutation increasing interaction strength mutation increasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 18:05:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:43:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' P43628:p.Thr105Pro mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T P Wed May 02 18:59:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr105pro' Tue Jun 30 17:06:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr105Pro' P43628:p.[Gln92_Asp93delinsProVal;Thr105Pro] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain QD PV certain certain sequence position certain certain certain sequence position certain T P Mon Feb 27 13:56:04 GMT 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_pro92-93pro_val,thr105pro' Wed May 02 18:51:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln_asp92-93pro_val,thr105pro' Tue Jun 30 20:07:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln92_Asp93delinsProVal;Thr105Pro]' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 3 unspecified role unspecified role bait bait gly115arg variant variant certain certain sequence position certain certain certain sequence position certain G R direct interaction direct interaction Fig 1, Supp Fig 3-12 C0303 allele G115R variant dbSNP:rs1131122 hla_c-kir2dl3-6 2 9 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 19:15:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 17:50:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 7 unspecified role unspecified role bait bait glu5ala variant variant certain certain sequence position certain certain certain sequence position certain E A asn92lys variant variant certain certain sequence position certain certain certain sequence position certain glu179gln variant variant certain certain sequence position certain certain certain sequence position certain E Q ser202thr variant variant certain certain sequence position certain certain certain sequence position certain S T ser364cys variant variant certain certain sequence position certain certain certain sequence position certain S C direct interaction direct interaction Fig 1, Supp Fig 3-14 C0102 allele KIR:-I cannot see that they have tested for the "T84" and "QD71-72PV +/-T84" mutants,and same for the rest of these results, -even after magnifying the image a lot. hla_c-kir2dl3-7 2 9 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 17:38:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 15:45:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 12 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-15 B7301 allele KIR:-I cannot see that they have tested for the "T84" and "QD71-72PV +/-T84" mutants,and same for the rest of these results, -even after magnifying the image a lot. hla_b-kir2dl3-1 2 9 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 16:28:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 18:30:55 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 12 unspecified role unspecified role bait bait direct interaction direct interaction Fig 1, Supp Fig 3-16 B4601 allele KIR:-I cannot see that they have tested for the "T84" and "QD71-72PV +/-T84" mutants,and same for the rest of these results, -even after magnifying the image a lot. hla_b-kir2dl3-2 2 9 unspecified role unspecified role prey prey region fc-igg tag fc-igg tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P43628:p.Gln92Pro mutation mutation certain certain sequence position certain certain certain sequence position certain Q P Wed May 02 16:01:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln92pro' Tue Jun 30 16:46:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln92Pro' trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. 10 unspecified role unspecified role bait bait glu43lys variant variant certain certain sequence position certain certain certain sequence position certain E K direct interaction direct interaction Fig 1, Supp Fig 3-17 A1101 and A1102 alleles I cannot see that they tested for the W.T.- so left KIR mutations as "mutations" - not "mutations causing"- but maybe I just cannot see the zero results? Text states:- "Mutating Gln71 to Pro71 in 2DL3 causes a gain of A*11 reactivity" -in both 01 and 02 alleles.