DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Nature 2009 imex curation Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions in vitro In vitro phosphatase assay phosphatase assay predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219615X Nature 2009 IMEx Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Nature 2009 imex curation Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219613X Nature 2009 IMEx Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Nature 2009 imex curation Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219612X Nature 2009 IMEx Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Structural basis of abscisic acid signalling. Nature 2009 imex curation Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Miyazono K., Miyakawa T., Sawano Y., Kubota K., Kang HJ., Asano A., Miyauchi Y., Takahashi M., Zhi Y., Fujita Y., Yoshida T., Kodaira KS., Yamaguchi-Shinozaki K., Tanokura M. DIP original experiment: DIP-219614X Nature 2009 IMEx Structural basis of abscisic acid signalling. pyl1_arath Abscisic acid receptor PYL1 At5g46790 PYL1 RCAR12 PYR1-like protein 1 ABI1-binding protein 6 Regulatory components of ABA receptor 9 MZA15.21 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MANSESSSSPVNEEENSQRISTLHHQTMPSDLTQDEFTQLSQSIAEFHTYQLGNGRCSSLLAQRIHAPPETVWSVVRRFDRPQIYKHFIKSCNVSEDFEMRVGCTRDVNVISGLPANTSRERLDLLDDDRRVTGFSITGGEHRLRNYKSVTTVHRFEKEEEEERIWTVVLESYVVDVPEGNSEEDTRLFADTVIRLNLQKLASITEAMNRNNNNNNSSQVR DIP protein Q8VZS8 original sequence version: 33 DIP protein Q8VZS8 original sequence version: 38 DIP protein Q8VZS8 original sequence version: 34 DIP protein Q8VZS8 original sequence version: 39 B64B1A52C43072C2 p2c56_arath Protein phosphatase 2C 56 Protein ABSCISIC ACID-INSENSITIVE 1 ABI1 At4g26080 F20B18.190 Protein phosphatase 2C ABI1 protein protein arath Arabidopsis thaliana (Mouse-ear cress) Mouse-ear cress MEEVSPAIAGPFRPFSETQMDFTGIRLGKGYCNNQYSNQDSENGDLMVSLPETSSCSVSGSHGSESRKVLISRINSPNLNMKESAAADIVVVDISAGDEINGSDITSEKKMISRTESRSLFEFKSVPLYGFTSICGRRPEMEDAVSTIPRFLQSSSGSMLDGRFDPQSAAHFFGVYDGHGGSQVANYCRERMHLALAEEIAKEKPMLCDGDTWLEKWKKALFNSFLRVDSEIESVAPETVGSTSVVAVVFPSHIFVANCGDSRAVLCRGKTALPLSVDHKPDREDEAARIEAAGGKVIQWNGARVFGVLAMSRSIGDRYLKPSIIPDPEVTAVKRVKEDDCLILASDGVWDVMTDEEACEMARKRILLWHKKNAVAGDASLLADERRKEGKDPAAMSAAEYLSKLAIQRGSKDNISVVVVDLKPRRKLKSKPLN DIP protein P49597 original sequence version: 104 DIP protein P49597 original sequence version: 101 DIP protein P49597 original sequence version: 106 DIP protein P49597 original sequence version: 102 DIP protein P49597 original sequence version: 116 DIP protein P49597 original sequence version: 107 DIP protein P49597 original sequence version: 124 DIP protein P49597 original sequence version: 161 4A4C54F04195F572 phosphopeptide Phosphopeptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) RPATVA abi1-pyl1-1 4 5 unspecified role unspecified role prey prey Region 8-211 Region 8-211 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8VZS8:p.His142Ala H142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain H A This feature has been corrected as a result of our quality control procedures. The original label was 'H142A'. Wed May 02 15:28:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'his142ala' Tue Jun 30 16:13:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.His142Ala' Q8VZS8:p.Leu144Ala L144A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A This feature has been corrected as a result of our quality control procedures. The original label was 'L144A'. Wed May 02 15:27:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu144ala' Tue Jun 30 17:21:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu144Ala' Q8VZS8:p.Arg143Ala R143A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R143A'. Wed May 02 17:29:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg143ala' Tue Jun 30 17:00:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg143Ala' Q8VZS8:p.Phe189Ala F189A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F189A'. Wed May 02 19:38:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe189ala' Tue Jun 30 19:00:12 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe189Ala' ecolx Escherichia coli 6 unspecified role unspecified role bait bait PP2C-domain Region 125-429 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P49597:p.Ile298Ala I298A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I298A'. Wed May 02 19:00:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile298ala' Tue Jun 30 15:53:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile298Ala' P49597:p.Trp300Ala W300A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W300A'. Wed May 02 17:59:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp300ala' Tue Jun 30 16:56:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp300Ala' P49597:p.Arg304Ala R304A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R304A'. Wed May 02 16:52:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg304ala' Tue Jun 30 17:34:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg304Ala' P49597:p.Glu142Ala E142A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E142A'. Wed May 02 17:35:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu142ala' Tue Jun 30 17:49:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu142Ala' P49597:p.Tyr319Ala Y319A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A This feature has been corrected as a result of our quality control procedures. The original label was 'Y319A'. Wed May 02 15:32:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr319ala' Tue Jun 30 18:44:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr319Ala' ecolx Escherichia coli direct interaction direct interaction abi1-phosphopeptide-1 1 7 enzyme target enzyme target substrate substrate unspecified role unspecified role in vitro In vitro 6 enzyme enzyme unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P49597:p.Glu142Ala E142A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E142A'. Wed May 02 18:15:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu142ala' Tue Jun 30 20:22:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu142Ala' ecolx Escherichia coli dephosphorylation dephosphorylation reaction dephosphorylation abi1-pyl1-2 3 5 unspecified role unspecified role unspecified role unspecified role Region 28-210 Region 28-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction abi1-pyl1-3 2 5 unspecified role unspecified role unspecified role unspecified role Region 28-210 Region 28-210 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role PP2C-domain Region 125-429 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction