DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. DIP original experiment: DIP-134571X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. DIP original experiment: DIP-134572X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. DIP original experiment: DIP-134573X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. Only protein-protein interactions in vitro In vitro pull down pull down autoradiography autoradiography Taniguchi N., Caramés B., Kawakami Y., Amendt BA., Komiya S., Lotz M. DIP original experiment: DIP-134570X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Chromatin protein HMGB2 regulates articular cartilage surface maintenance via beta-catenin pathway. lef1_mouse Lymphoid enhancer-binding factor 1 Lef1 Lef-1 protein protein mouse Mus musculus Mouse MPQLSGGGGGGDPELCATDEMIPFKDEGDPQKEKIFAEISHPEEEGDLADIKSSLVNESEIIPASNGHEVVRQAPSSQEPYHDKAREHPDEGKHPDGGLYNKGPSYSSYSGYIMMPNMNSDPYMSNGSLSPPIPRTSNKVPVVQPSHAVHPLTPLITYSDEHFSPGSHPSHIPSDVNSKQGMSRHPPAPEIPTFYPLSPGGVGQITPPIGWQGQPVYPITGGFRQPYPSSLSGDTSMSRFSHHMIPGPPGPHTTGIPHPAIVTPQVKQEHPHTDSDLMHVKPQHEQRKEQEPKRPHIKKPLNAFMLYMKEMRANVVAECTLKESAAINQILGRRWHALSREEQAKYYELARKERQLHMQLYPGWSARDNYGKKKKRKREKLQESTSGTGPRMTAAYI DIP protein P27782 original sequence version: 88 DIP protein P27782-1 original sequence version: 103 0CA01B6C528FD7FA ctnb1_human Catenin beta-1 Beta-catenin OK/SW-cl.35 PRO2286 CTNNB1 CTNNB protein protein human Homo sapiens Human MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEEEDVDTSQVLYEWEQGFSQSFTQEQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDAAHPTNVQRLAEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSKKEASRHAIMRSPQMVSAIVRTMQNTNDVETARCTAGTLHNLSHHREGLLAIFKSGGIPALVKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDCLQILAYGNQESKLIILASGGPQALVNIMRTYTYEKLLWTTSRVLKVLSVCSSNKPAIVEAGGMQALGLHLTDPSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCAAGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEMAQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLLVRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFVQLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLFRMSEDKPQDYKKRLSVELTSSLFRTEPMAWNETADLGLDIGAQGEPLGYRQDDPSYRSFHSGGYGQDALGMDPMMEHEMGGHHPGADYPVDGLPDLGHAQDLMDGLPPGDSNQLAWFDTDL DIP protein P35222 original sequence version: 187 DIP protein P35222 original sequence version: 188 DIP protein P35222 original sequence version: 180 DIP protein P35222-1 original sequence version: 181 DIP protein P35222 original sequence version: 181 DIP protein P35222 original sequence version: 107 DIP protein P35222-1 original sequence version: 107 DIP protein P35222 original sequence version: 103 DIP protein P35222-1 original sequence version: 103 DIP protein P35222 original sequence version: 175 DIP protein P35222 original sequence version: 136 DIP protein P35222 original sequence version: 135 DIP protein P35222 original sequence version: 131 DIP protein P35222-1 original sequence version: 133 DIP protein P35222 original sequence version: 133 DIP protein P35222 original sequence version: 148 DIP protein P35222-1 original sequence version: 139 DIP protein P35222 original sequence version: 139 DIP protein P35222 original sequence version: 146 DIP protein P35222 original sequence version: 196 DIP protein P35222 original sequence version: 192 DIP protein P35222 original sequence version: 185 DIP protein P35222 original sequence version: 151 DIP protein P35222-1 original sequence version: 151 DIP protein P35222-1 original sequence version: 171 DIP protein P35222-1 original sequence version: 148 DIP protein P35222 original sequence version: 177 DIP protein P35222 original sequence version: 178 DIP protein P35222-1 original sequence version: 178 CB78F165A3EEF86E hmgb2_mouse High mobility group protein B2 Hmgb2 Hmg2 High mobility group protein 2 protein protein mouse Mus musculus Mouse MGKGDPNKPRGKMSSYAFFVQTCREEHKKKHPDSSVNFAEFSKKCSERWKTMSAKEKSKFEDLAKSDKARYDREMKNYVPPKGDKKGKKKDPNAPKRPPSAFFLFCSENRPKIKIEHPGLSIGDTAKKLGEMWSEQSAKDKQPYEQKAAKLKEKYEKDIAAYRAKGKSEAGKKGPGRPTGSKKKNEPEDEEEEEEEEEEEDDEEEEEDEE DIP protein P30681 original sequence version: 94 45D1F667DB4ED94D lef1-hmgb2-1 4 7 unspecified role unspecified role prey prey A-box linker domain Region 1-94 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain B-box linker domain Region 79-162 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction hmgb2-lef1-1 2 7 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey HMG domain Region 296-397 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction lef1-ctnnb1-7 1 6 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction ctnnb1-lef1-9 3 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey 35S-label 35S-label 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction