DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. Only protein-protein interactions nicbe Nicotiana benthamiana molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. DIP original experiment: DIP-134577X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. Only protein-protein interactions sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. DIP original experiment: DIP-134575X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. Only protein-protein interactions sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Ottmann C., Rose R., Huttenlocher F., Cedzich A., Hauske P., Kaiser M., Huber R., Schaller A. DIP original experiment: DIP-134576X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Structural basis for Ca2+-independence and activation by homodimerization of tomato subtilase 3. sbt3_sollc Subtilisin-like protease SBT3 sbt3 LeSBT3 SlSBT3 Subtilase 3 Solyc01g087850 protein protein sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato MELLHLLLFSWALSAHLFLALAQRSTYIVHLDKSLMPNVFTDHHHWHSSTIDSIKASVPSSVDRFHSAPKLVYSYDNVLHGFSAVLSKDELAALKKLPGFISAYKDRTVEPHTTHTSDFLKLNPSSGLWPASGLGQDVIVAVLDSGIWPESASFQDDGMPEIPKRWKGICKPGTQFNASMCNRKLIGANYFNKGILANDPTVNITMNSARDTDGHGTHCASITAGNFAKGVSHFGYAPGTARGVAPRARLAVYKFSFNEGTFTSDLIAAMDQAVADGVDMISISYGYRFIPLYEDAISIASFGAMMKGVLVSASAGNRGPGIGSLNNGSPWILCVASGHTDRTFAGTLTLGNGLKIRGWSLFPARAFVRDSPVIYNKTLSDCSSEELLSQVENPENTIVICDDNGDFSDQMRIITRARLKAAIFISEDPGVFRSATFPNPGVVVNKKEGKQVINYVKNSVTPTATITFQETYLDTKPAPVVAASSARGPSRSYLGISKPDILAPGVLILAAYPPNVFATSIGTNILLSTDYILESGTSMAAPHAAGIAAMLKAAHPEWSPSAIRSAMMTTADPLDNTRKPIKDSDNNKAATPLDMGAGHVDPNRALDPGLVYDATPQDYVNLLCSLNFTEEQFKTIARSSASHNCSNPSADLNYPSFIALYSIEGNFTLLEQKFKRTVTNVGKGAATYKAKLKAPKNSTISVSPQILVFKNKNEKQSYTLTIRYIGDEGQSRNVGSITWVEQNGNHSVRSPIVTSPIIEVW DIP protein O82777 original sequence version: 52 sbt3-1 2 4 unspecified role unspecified role unspecified role unspecified role Mature protein Region 113-761 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato direct interaction direct interaction sbt3-2 3 4 unspecified role unspecified role unspecified role unspecified role Mature protein Region 113-761 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain sollc Solanum lycopersicum (Tomato) Lycopersicon esculentum Tomato direct interaction direct interaction sbt3-3 1 4 unspecified role unspecified role unspecified role unspecified role Mature protein Region 113-761 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain nicbe Nicotiana benthamiana direct interaction direct interaction