DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. DIP original experiment: DIP-134446X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Furgason ML., MacDonald C., Shanks SG., Ryder SP., Bryant NJ., Munson M. DIP original experiment: DIP-134445X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx The N-terminal peptide of the syntaxin Tlg2p modulates binding of its closed conformation to Vps45p. vps45_yeast Vacuolar protein sorting-associated protein 45 VPS45 STT10 VPL28 YGL095C protein protein yeast Saccharomyces cerevisiae Baker's yeast MNLFDVADFYINKIVTSQSKLSVANVNEHQRIKVLLLDKNTTPTISLCATQSELLKHEIYLVERIENEQREVSRHLRCLVYVKPTEETLQHLLRELRNPRYGEYQIFFSNIVSKSQLERLAESDDLEAVTKVEEIFQDFFILNQDLFSFDLQPREFLSNKLVWSEGGLTKCTNSLVSVLLSLKIKPDIRYEGASKICERLAKEVSYEIGKNERTFFDFPVMDSTPVLLILDRNTDPITPLLQPWTYQSMINEYIGIKRNIVDLSKVPRIDKDLEKVTLSSKQDAFFRDTMYLNFGELGDKVKQYVTTYKDKTQTNSQINSIEDIKNFIEKYPEFRKLSGNVAKHMAIVGELDRQLKIKNIWEISEIEQNLSAHDANEEDFSDLIKLLQNEAVDKYYKLKLACIYSLNNQTSSDKIRQLVEILSQQLPPEDVNFFHKFKSLFSRQDKMTQSNHDKDDILTELARRFNSRMNSKSNTAENVYMQHIPEISSLLTDLSKNALFRDRFKEIDTQGHRVIGNQQSKDIPQDVILFVIGGVTYEEARLVHDFNGTMNNRMRVVLGGTSILSTKEYMDSIRSAK 4AA50D568228228F 4AA50D568228228F tlg2_yeast t-SNARE affecting a late Golgi compartment protein 2 YOL018C TLG2 Syntaxin TLG2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFRDRTNLFLSYRRTFPHNITFSSGKAPLGDDQDIEMGTYPMMNMSHDISARLTDERKNKHENHSDALPPIFIDIAQDVDDYLLEVRRLSEQLAKVYRKNSLPGFEDKSHDEALIEDLSFKVIQMLQKCYAVMKRLKTIYNSQFVDGKQLSREELIILDNLQKIYAEKIQTESNKFRVLQNNYLKFLNKDDLKPIRNKASAENTLLLDDEEEEAAREKREGLDIEDYSKRTLQRQQQLHDTSAEAYLRERDEEITQLARGVLEVSTIFREMQDLVVDQGTIVDRIDYNLENTVVELKSADKELNKATHYQKRTQKCKVILLLTLCVIALFFFVMLKPHGGGSGGRNNGSNKYNNDDNKTVNNSHDDGSNTHINDEESNLPSIVEVTESENDALDDLL CEBD6FE11278B1F0 CEBD6FE11278B1F0 tlg2p-vps45p-1 2 4 unspecified role unspecified role unspecified role unspecified role Region 37-318 Region 37-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction tlg2p-vps45p-2 1 4 unspecified role unspecified role unspecified role unspecified role Region 37-318 Region 37-318 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Alexa488-label Alexa488-label alexa 488 label alexa 488 label certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction