UniProt UniProt http://www.uniprot.org GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. American journal of physiology. Heart and circulatory physiology 2009 imex curation Valdivia CR., Ueda K., Ackerman MJ., Makielski JC. Jcm@medicine.wisc.edu Only protein-protein interactions in vitro In vitro oxidoreduct assay oxidoreductase assay oxidoreduct assay predetermined predetermined participant predetermined Valdivia CR., Ueda K., Ackerman MJ., Makielski JC. Jcm@medicine.wisc.edu jcm@medicine.wise.edu Accepted 2017-FEB-23 AT 11:13 GMT AT 11:13 GMT by ORCHARD American journal of physiology. Heart and circulatory physiology 2009 imex curation GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. GPD1L links redox state to cardiac excitability by PKC-dependent phosphorylation of the sodium channel SCN5A. American journal of physiology. Heart and circulatory physiology 2009 imex curation Valdivia CR., Ueda K., Ackerman MJ., Makielski JC. Jcm@medicine.wisc.edu Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Valdivia CR., Ueda K., Ackerman MJ., Makielski JC. Jcm@medicine.wisc.edu jcm@medicine.wise.edu Accepted 2017-FEB-23 AT 11:13 GMT AT 11:13 GMT by ORCHARD American journal of physiology. Heart and circulatory physiology 2009 imex curation gpd1l_human Glycerol-3-phosphate dehydrogenase 1-like protein GPD1L KIAA0089 protein protein human Homo sapiens Human MAAAPLKVCIVGSGNWGSAVAKIIGNNVKKLQKFASTVKMWVFEETVNGRKLTDIINNDHENVKYLPGHKLPENVVAMSNLSEAVQDADLLVFVIPHQFIHRICDEITGRVPKKALGITLIKGIDEGPEGLKLISDIIREKMGIDISVLMGANIANEVAAEKFCETTIGSKVMENGLLFKELLQTPNFRITVVDDADTVELCGALKNIVAVGAGFCDGLRCGDNTKAAVIRLGLMEMIAFARIFCKGQVSTATFLESCGVADLITTCYGGRNRRVAEAFARTGKTIEELEKEMLNGQKLQGPQTSAEVYRILKQKGLLDKFPLFTAVYQICYESRPVQEMLSCLQSHPEHT 74C3B27EEB41DE89 sn-glycerol 3-phosphate sn-glycerol 3-phosphate (R)-glycerol 1-phosphate D-(glycerol 1-phosphate) Glycerol-3-phosphate Glycerophosphoric acid L-(glycerol 3-phosphate) phosphoric acid mono-((R)-2,3-dihydroxy-propyl) ester Phosphorsaeure-mono-((R)-2,3-dihydroxy-propylester) sn-Glycerol 3-phosphate SN-GLYCEROL-3-PHOSPHATE sn-Gro-1-P (2R)-2,3-dihydroxypropyl dihydrogen phosphate sn-glycerol 3-(dihydrogen phosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) AWUCVROLDVIAJX-GSVOUGTGSA-N AWUCVROLDVIAJX-GSVOUGTGSA-N scn5a_human Sodium channel protein type 5 subunit alpha Sodium channel protein type V subunit alpha Voltage-gated sodium channel subunit alpha Nav1.5 Sodium channel protein cardiac muscle subunit alpha SCN5A hH1 protein protein human Homo sapiens Human MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSTTLQESREGLPEEEAPRPQLDLQASKKLPDLYGNPPQELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPIRRAAVKILVHSLFNMLIMCTILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIIMAYTTEFVDLGNVSALRTFRVLRALKTISVISGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKCVRNFTALNGTNGSVEADGLVWESLDLYLSDPENYLLKNGTSDVLLCGNSSDAGTCPEGYRCLKAGENPDHGYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQNQATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVNSHERRSKRRKRMSSGTEECGEDRLPKSDSEDGPRAMNHLSLTRGLSRTSMKPRSSRGSIFTFRRRDLGSEADFADDENSTAGESESHHTSLLVPWPLRRTSAQGQPSPGTSAPGHALHGKKNSTVDCNGVVSLLGAGDPEATSPGSHLLRPVMLEHPPDTTTPSEEPGGPQMLTSQAPCVDGFEEPGARQRALSAVSVLTSALEELEESRHKCPPCWNRLAQRYLIWECCPLWMSIKQGVKLVVMDPFTDLTITMCIVLNTLFMALEHYNMTSEFEEMLQVGNLVFTGIFTAEMTFKIIALDPYYYFQQGWNIFDSIIVILSLMELGLSRMSNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKNYSELRDSDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCMEVSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDREMNNLQLALARIQRGLRFVKRTTWDFCCGLLRQRPQKPAALAAQGQLPSCIATPYSPPPPETEKVPPTRKETRFEEGEQPGQGTPGDPEPVCVPIAVAESDTDDQEEDEENSLGTEEESSKQQESQPVSGGPEAPPDSRTWSQVSATASSEAEASASQADWRQQWKAEPQAPGCGETPEDSCSEGSTADMTNTAELLEQIPDLGQDVKDPEDCFTEGCVRRCPCCAVDTTQAPGKVWWRLRKTCYHIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLKWVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNALVGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSQCESLNLTGELYWTKVKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEYNLYMYIYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFLICLNMVTMMVETDDQSPEKINILAKINLLFVAIFTGECIVKLAALRHYYFTNSWNIFDFVVVILSIVGTVLSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMANFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPTLPNSNGSRGDCGSPAVGILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYSVLSDFADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKISYEPITTTLRRKHEEVSAMVIQRAFRRHLLQRSLKHASFLFRQQAGSGLSEEDAPEREGLIAYVMSENFSRPLGPPSSSSISSTSFPPSYDSVTRATSDNLQVRGSDYSHSEDLADFPPSPDRDRESIV DIP protein Q14524 original sequence version: 163 DIP protein Q14524 original sequence version: 119 DIP protein Q14524 original sequence version: 138 This sequence has been withdrawn from Uniprot. 841E3A365931190B gpd1l-scn5a-1 2 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8N335:p.Glu83Lys mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain E K Wed May 02 14:39:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu83lys' Tue Jun 30 17:57:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu83Lys' Q8N335:p.Ala280Val mutation with no effect mutation with no effect mutation with no effect mutation not affecting interaction certain certain sequence position certain certain certain sequence position certain A V Wed May 02 15:20:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala280val' Tue Jun 30 15:47:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala280Val' ecoli Escherichia coli (strain K12) glu83lys and ala280val mutant constucts also interact 5 unspecified role unspecified role prey prey cell lysate cell lysate human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. physical association physical association 5 gpd1l-sn_glycerol 3_phosphate-1 1 3 unspecified role unspecified role bait bait n-terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8N335:p.Glu83Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain E K Wed May 02 16:43:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu83lys' Tue Jun 30 16:33:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu83Lys' Q8N335:p.Ala280Val mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain A V Wed May 02 19:34:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ala280val' Tue Jun 30 18:49:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ala280Val' ecoli Escherichia coli (strain K12) glu83lys and ala280val mutant constucts also interact 4 unspecified role unspecified role prey prey human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. redox reaction oxidoreductase activity electron transfer reaction redox reaction Fig. 2A