DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Nature structural & molecular biology 2009 imex curation Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133092X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Nature structural & molecular biology 2009 imex curation Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133090X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. Nature structural & molecular biology 2009 imex curation Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. Only protein-protein interactions in vitro In vitro protease assay protease assay predetermined predetermined participant predetermined Agarwal R., Schmidt JJ., Stafford RG., Swaminathan S. DIP original experiment: DIP-133091X Nature structural & molecular biology 2009 IMEx Mode of VAMP substrate recognition and inhibition of Clostridium botulinum neurotoxin F. vamp2_human Vesicle-associated membrane protein 2 Synaptobrevin-2 VAMP2 SYB2 protein protein human Homo sapiens Human MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST DIP protein P63027 original sequence version: 108 DIP protein P63027 original sequence version: 60 DIP protein P63027 original sequence version: 74 9CD679C4F6F1B5A8 q57236_clobo F bonT protein protein clobo Clostridium botulinum MPVVINSFNYNDPVNDDTILYMQIPYEEKSKKYYKAFEIMRNVWIIPERNTIGTDPSDFDPPASLENGSSAYYDPNYLTTDAEKDRYLKTTIKLFKRINSNPAGEVLLQEISYAKPYLGNEHTPINEFHPVTRTTSVNIKSSTNVKSSIILNLLVLGAGPDIFENSSYPVRKLMDSGGVYDPSNDGFGSINIVTFSPEYEYTFNDISGGYNSSTESFIADPAISLAHELIHALHGLYGARGVTYKETIKVKQAPLMIAEKPIRLEEFLTFGGQDLNIITSAMKEKIYNNLLANYEKIATRLSRVNSAPPEYDINEYKDYFQWKYGLDKNADGSYTVNENKFNEIYKKLYSFTEIDLANKFKVKCRNTYFIKYGFLKVPNLLDDDIYTVSEGFNIGNLAVNNRGQNIKLNPKIIDSIPDKGLVEKIVKFCKSVIPRKGTKAPPRLCIRVNNRELFFVASESSYNENDINTPKEIDDTTNLNNNYRNNLDEVILDYNSETIPQISNQTLNTLVQDDSYVPRYDSNGTSEIEEHNVVDLNVFFYLHAQKVPEGETNISLTSSIDTALSEESQVYTFFSSEFINTINKPVHAALFISWINQVIRDFTTEATQKSTFDKIADISLVVPYVGLALNIGNEVQKENFKEAFELLGAGILLEFVPELLIPTILVFTIKSFIGSSENKNKIIKAINNSLMERETKWKEIYSWIVSNWLTRINTQFNKRKEQMYQALQNQVDAIKTVIEYKYNNYTSDERNRLESEYNINNIREELNKKVSLAMENIERFITESSIFYLMKLINEAKVSKLREYDEGVKEYLLDYISEHRSILGNSVQELNDLVTSTLNNSIPFELSSYTNDKILILYFNKLYKKIKDNSILDMRYENNKFIDISGYGSNISINGDVYIYSTNRNQFGIYSSKPSEVNIAQNNDIIYNGRYQNFSISFWVRIPKYFNKVNLNNEYTIIDCIRNNNSGWKISLNYNKIIWTLQDTAGNNQKLVFNYTQMISISDYINKWIFVTITNNRLGNSRIYINGNLIDEKSISNLGDIHVSDNILFKIVGCNDTRYVGIRYFKVFDTELGKTEIETLYSDEPDPSILKDFWGNYLLYNKRYYLLNLLRTDKSITQNSNFLNINQQRGVYQKPNIFSNTRLYTGVEVIIRKNGSTDISNTDNFVRKNDLAYINVVDRDVEYRLYADISIAKPEKIIKLIRTSNSNNSLGQIIVMDSIGNNCTMNFQNNNGGNIGLLGFHSNNLVASSWYYNNIRKNTSSNGCFWSFISKEHGWQEN DIP protein Q57236 original sequence version: 58 bont-vamp2-1 2 4 unspecified role unspecified role unspecified role unspecified role Region 27-58 Region 27-58 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction bont-vamp2-2 1 4 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-97 Region 1-97 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P63027:p.[Val53Asp;Leu54Asp] V53D/L54D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L D certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V53D/L54D'. Wed May 02 18:14:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val53asp,leu54asp' Tue Jun 30 16:56:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val53Asp;Leu54Asp]' P63027:p.Val53Asp V53D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V53D'. Wed May 02 16:50:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val53asp' Tue Jun 30 18:01:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val53Asp' P63027:p.Val50Asp V50D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V D This feature has been corrected as a result of our quality control procedures. The original label was 'V50D'. Wed May 02 19:49:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val50asp' Tue Jun 30 19:58:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val50Asp' P63027:p.Glu41Ala E41A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E41A'. Wed May 02 16:33:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu41ala' Tue Jun 30 18:23:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu41Ala' ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli protein cleavage protein cleavage bont-vamp2-3 3 4 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-94 Region 1-94 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role Region 1-416 Region 1-416 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q57236:p.Arg133Ala R133A/K mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Wed May 02 15:54:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg133ala' Tue Jun 30 19:48:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg133Ala' Q57236:p.Glu164Ala E164A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E164A'. Wed May 02 15:16:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu164ala' Tue Jun 30 19:06:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu164Ala' Q57236:p.Arg171Ala R171A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R171A'. Wed May 02 16:32:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg171ala' Tue Jun 30 16:38:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg171Ala' Q57236:p.Arg133Lys mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain R K Wed May 02 18:01:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg133lys' Tue Jun 30 17:26:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg133Lys' ecolx Escherichia coli protein cleavage protein cleavage