DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249927X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249926X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249929X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249924X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249928X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. Nature 2009 imex curation Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. Only protein-protein interactions ecolx Escherichia coli dls dynamic light scattering dls predetermined predetermined participant predetermined Barends TR., Hartmann E., Griese JJ., Beitlich T., Kirienko NV., Ryjenkov DA., Reinstein J., Shoeman RL., Gomelsky M., Schlichting I. DIP original experiment: DIP-249925X Nature 2009 IMEx Structure and mechanism of a bacterial light-regulated cyclic nucleotide phosphodiesterase. a6t8v8_klep7 KPN_01598 protein protein klep7 Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) MLTTLIYRSQVHPDRPPVDLDALVHRASSKNLPLGITGILLFNGLQFFQVLEGTEEALESLFSEIQSDPRHRDVVELMRDYSAYRRFHGTGMRILDLRLFETDGALEEILRFSTFGVTEPVNDRMFRLLSAFIADGGRYCLPEPLQPSRWMMMPASGTAAPQHLPGQPCQFALQAIVEPAKKRVSSFEALIRSPTGGSPVEMFAAIAAEDRYRFDLESKAYAFALAGQLPLGKHQLAINLLPGSLYHHPDAVGWLMDSLLAAGLRPDQVLIEVTETEVITCFDQFRKVLKALRVAGMKLAIDDFGAGYSGLSLLTRFQPDKIKVDAELVRDIHISGTKQAIVASVVRCCEDLGITVVAEGVETLEEWCWLQSVGIRLFQGFLFSRPCLNGIGEICWPVARQAMDL DIP protein A6T8V8 original sequence version: 33 q2ld83_ecolx ELT33_13095 A5U30_002913 CT143_20570 DXB10_24210 GF147_06695 HMV41_19180 MS7163_01245 BvCmsSIP024_03948 EWK56_18500 ycgF ycgF_1 AZZ83_000918 NCTC10767_03158 DS966_14890 NCTC9434_03761 bluF D1912_30690 D3C88_06535 BON91_15120 EQO00_14400 D4U49_02820 EA239_21370 FPI65_06905 protein protein ecolx Escherichia coli MLTTLIYRSHIRGDEPVKKIEEMVSIANRRNMRSDVTGILLFNGSHFFQLLEGPEEQVQMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHERDDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKELDSCTLSSNINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDEEIYTADLKSKALAFAMAHALKLGDKMISINLLPMTLVNKPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAVKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIMKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFSKAKLNGIPSVAWPEKK DIP protein Q2LD83 original sequence version: 24 ycgf-1 5 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a6t8v8_klep7-1 4 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ycgf-2 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a6t8v8_klep7-2 6 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a6t8v8_klep7-3 2 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction a6t8v8_klep7-4 1 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction