DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249912X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249909X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip ms participant Identification by mass spectrometry ms participant Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249910X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249911X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249907X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249913X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. A spatial gradient coordinates cell size and mitotic entry in fission yeast. Nature 2009 imex curation Moseley JB., Mayeux A., Paoletti A., Nurse P. Only protein-protein interactions schpm Schizosaccharomyces pombe (fission yeast) Fission yeast anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Moseley JB., Mayeux A., Paoletti A., Nurse P. DIP original experiment: DIP-249908X Nature 2009 IMEx A spatial gradient coordinates cell size and mitotic entry in fission yeast. blt1_schpo Mitosis inducer protein blt1 blt1 SPBC1A4.05 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSKSAFTSKSQLKGIDGSFNDPFRQPSMNLPTPPHDDGLPSIPRSTALPNLSNRLSPYSHRNYLPIPEADSRNLEVLNSISLTTGSVVNQINKLYQRSCDNANYLQDLRSLILQTPTAEANATQVTESLNQIQKILQEASSKDREQIVQVIKELNKGNSLEVRRELGNCLAHLKKGTLNIDNALQASLQKYWKELQYFNVSKDKLLSLEESIKLLSARLEETDTPSSTHWIEINAGFKEVGDELSENFQRKQEILSGLQNNVILRTNNRKPVGSDGSNSNFNGGEEQMDSKDQEEIQDYLNSLLSVHEKDGVLLQKFHNSYVQYQKLAVALSKYENFDADKLFQQMNLAKQSNETLLQTLTEQTDQLLSFKETMDSFKFILGPSMENQALQQGILAEQQDLVAQLRDIVLRSETLAENPSNMPGSCLPGASSNTADEFTEQLNLLKNEVARLSAICPSPNSGINASVLTNADNLEKENLLLVNNNAFKVDDRSVSSVALDDHNRQLQMNVEELEGKKADLTSKINRLDKDFVKLNTTYSLLTDQVKTKQLALQEIESRVIRLEERLNMLQKLSMQPAISSSSEFVPIESHPSSSAVVPIEEPKNSIIEKKRVPHNAARNSVNLEVTLPNSKKRFSSFSGSSSKLPVRPSTALTDKRKPSWSRRLAAAIGFSSGSPEKKHVITSSDAGHQRSKSRSFSSKM 0A55373AFC35E77E 0A55373AFC35E77E cdr2_schpo Mitosis inducer protein kinase cdr2 cdr2 SPAC57A10.02 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTISEVGPWELGLSLGSGGPNSSRLAKHRETGQLAVVKPIVGWSELTSSQQARIEGELVLLRLIEHPNVLQLIDVISAQEQLFVVVEYMPGGELFDCMLRKGSFTEQDTAKFLWQILCGLEYCHKLHICHRDLKPENLYLDAHGSIKIGEFGMASIQQPGKLLQTSCGSPHYASPEIIMGRSYDGCASDIWSCGIIFFALLTGKLPFDDDNIRSLLLKVCQGQFEMPSNISPQAQHLLYRMLDVDSSTRITMEQIREHPFLSCFVHPNISIPIISAPIQPIDPLIVQHLSLVFRCSDDPMPLYEKLASQSPLVEKTLYTLLSRHLHPPSSAAVDRNRAVVDDLLGTAASNGQQMDEEEIEQAINIPTLAPYPISYAAESVPRPATSASPFLTPVTTSGTFNYSFNATNPQSILQRPATTSSAVPQLPKSVTPGLAYPHDSSMLSSNYRPPSALSPRNFNVSINDPEVQLSRRATSLDMSNDFRMNENDPSIVGNLAASNFPTGMGPPRKRVTSRMSEHTGNRVVSFPRGSAFNPRVTRFNVGNEQFSNNIDNNNYNQPYANATMNNSRRLRTPSGERSMRADLSQSPASYDSLNVPKHRRRQSLFSPSSTKKKLSGSPFQPKRSFLRRLFSSEPSCKCVYASLVASELEHEILEVLRRWQLLGIGIADIIYDSVSASISARIKRQNSLNLKPVRFRISVLAEFFGSQAVFVLESGSSTTFDHLATEFQLIFEDKGFLDNLELSYFQASASRPVSRMSVSSSPFAVFRQRQSVQS 2EE2EECABC8A4FAC 2EE2EECABC8A4FAC klp8_schpo Kinesin-like protein 8 klp8 SPAC144.14 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MSTANVRVIVRVRPKSLRELSKSAEDLLSVDSHNKTVTITPPKHGLKHSRHKNRVNGPRTFAFDECFAPSAPESKNLSGQEDVYESTGPLLVKSILEGFNSCFITYGQKGTGKTYSVVGLRGQPGIIPHISESIFEEIDKLKKKSPNTTITVSISLAEIIEETPYDLLQPNVNSSHTPGETVFVQKDSLTGYHLHGLSEFEVGSAQEIDAFLRLAAKNIRTELSDISGVRKGHSVFSLVVQQRIIDPKTRHSLKKASRLQIIDLASFSKGSQRNESISSFESSSNNKSLSVLNRVIAALTSKKNDVLIPYKDSVLTTLLQDALGGNCRTIMLTCVSPCDFDDTFSALRYSEAARRIKNISNINCKEAYSTNNEGELDDILTTLESDREQLRRHEEHSQKLLKFIEEIRNDYEERIHALESQNSALKAHLRLAVDAYLNPLEFNFDDKNVKLKEFGSPNIAYKQELNSFQGELSSLFKDLKLVKSQLHDYPKPEVSDIDMDMESLRHDSLLD F6ED1C9ABBA28CE1 mid1_schpo Anillin-related medial ring protein mid1 mid1 dmf1 SPCC4B3.15 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MKEQEFSYREAKDVSLDSKGLENSFLSSPNREKTPLFFEGNSNETSGYDQTKNFTHGDGDMSLGNLSELNVATDLLESLDLRSMYMHGYGHLDSSFSSQHSPDNRKRMSSTSVFKRINSEEEGRIPSLTYSAGTMNSTSSSTASLKGADIVADYETFNPDQNLAELSFDRSKSSRKRAVEVAEFSRAKTMSPLEYTVQHPYQSHNELSTNPARARAGSVPNLARIPSDVKPVPPAHLSASSTVGPRILPSLPKDTTEDNPALERVETTASLDMDYKPLEPLAPIQEAPVEDTSEPFSSVPEATLDDSDISTESLRKKVLAKMEAKRISSGSSYASTLRKVYDFSELSLPTNGKDYDELYLQSSRNSEPEISTIINDSLQQENMDEDISATSIPKSQAAYGHGSVTYHEVPRYNLTSASVGYSISSQRGRIKSSSTIDNLSAILSSEDLRHPSMQPVPGTKRTYSNYCENEPNKSSQSLVSSESHNVEGWNYSETGTVGFYDPSAEISASIDELRQSTPVARDSELLSRAHSFDLNRLDLPSQDKSTSYEVPNGTENQSPRPVTSLGFVNETFFEEKPKAPLPLGRFYIHLNSILNISISEVHSPIKIIVNTPTQNMQLPWQAVNGNNRLDHDFAFHVDDNFKVSFMFLDIPIEDKSNGSKGVSATKDVSNGKPAETKSKARKFFDKLFNRRKKRKLNKAAAVENSKAKKSVVIKKVSGTATLNLGNVKDSCFGKAFNVEIPIISRGFLEAIPVKINSIGKRTLGNLTLTCLYIPELSVPEQELPFTLEQATMDLRHVRSNYLYNEGYLYRLEDSSIRRRFVVLRSKQLNFYAEKGGQYLDTFQLSKTVVSIPMVNFSEAVSNLGLVAGILATSVDRRHVQLFADSKKVCQKWLQVMNSRSFALDRGTEKLWLQEYVNFMA CA95FB48A20549B5 CA95FB48A20549B5 cdc15_schpo Cell division control protein 15 cdc15 SPAC20G8.05c protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MEVNGVSQSEAAPYVTKSSVKFRDNFWGSEDAGMDALMSRTKSSLSVLESIDEFYAKRASIEREYASKLQELAASSADIPEVGSTLNNILSMRTETGSMAKAHEEVSQQINTELRNKIREYIDQTEQQKVVAANAIEELYQKKTALEIDLSEKKDAYEYSCNKLNSYMRQTKKMTGRELDKYNLKIRQAALAVKKMDAEYRETNELLLTVTREWIDRWTEVCDAFQHIEEYRLEFLKTNMWAYANIISTACVKDDESCEKIRLTLENTNIDEDITQMIQNEGTGTTIPPLPEFNDYFKENGLNYDIDQLISKAPSYPYSSSRPSASASLASSPTRSAFRPKTSETVSSEVVSSPPTSPLHSPVKPVSNEQVEQVTEVELSIPVPSIQEAESQKPVLTGSSMRRPSVTSPTFEVAARPLTSMDVRSSHNAETEVQAIPAATDISPEVKEGKNSENAITKDNDDIILSSQLQPTATGSRSSRLSFSRHGHGSQTSLGSIKRKSIMERMGRPTSPFMGSSFSNMGSRSTSPTKEGFASNQHATGASVQSDELEDIDPRANVVLNVGPNMLSVGEAPVESTSKEEDKDVPDPIANAMAELSSSMRRRQSTSVDDEAPVSLSKTSSSTRLNGLGYHSRNTSIASDIDGVPKKSTLGAPPAAHTSAQMQRMSNSFASQTKQVFGEQRTENSARESLRHSRSNMSRSPSPMLSRRSSTLRPSFERSASSLSVRQSDVVSPAPSTRARGQSVSGQQRPSSSMSLYGEYNKSQPQLSMQRSVSPNPLGPNRRSSSVLQSQKSTSSNTSNRNNGGYSGSRPSSEMGHRYGSMSGRSMRQVSQRSTSRARSPEPTNRNSVQSKNVDPRATFTAEGEPILGYVIALYDYQAQIPEEISFQKGDTLMVLRTQEDGWWDGEIINVPNSKRGLFPSNFVQTV FDCE7E0AAA3D247D FDCE7E0AAA3D247D gef2_schpo Rho guanine nucleotide exchange factor gef2 gef2 SPAC31A2.16 protein protein schpo Schizosaccharomyces pombe (strain ATCC 38366 / 972) Fission yeast MDVGTMGSRAELLAISIQFGGLWVFRDLLQMNPLFEQDHVFCTFNHPNMGLVLISFAKVDGHILAAYALSKLGPDTFSICYPFSSKLDAFAQTLDLEWQIASMHKEAAACVYEFLCVESVCLHTGENWRESVIEPEYASYIFDTINVFQSSSLTRELYSLGEPNGVREFVVDAIFDIKVDNSWWEDPSNSYWKTVIGSREMFEDSRKKTSSPSPSFASSKDAGTIPAIQKKKSLLIEMMETESTYVERLRHLVNDYALPLRDLAKSSKKLLGLYELNTLFPPCLNKLIQLNSAFLDEFEAIMSDLNFEDIDEKKFEEIDLRLACCFESHFFAFSQHYPRYLEQSNDFGNVLKMASKIPKFVEFHDQVKLNANMNVGLSQLIMEPVQRIPRYSLFLDQIILLTQEGECQHTYVRSVEIIKNIAEMPTVDAEERSRIFAGLQHIIPDLAPNMISNSRNFIDCIDVTREFLKNGQLHLIPYTLILFNDRICLVQRRSKSSIASTILDLRKQNPRNSYSKEKRAQYIGSNMNEAVELTRSMVEENTIFLISKYASSPSFFNEYPILKFRCDFENVRTMDRFYQSFQKALSMNKSQPSCLSFSKLNDFVVFFNNYSRFEYEKESKRSDIVCICTNDANVDKHKFLQDGNIVITFFQQDEDFHLSFDSWLGVSLPTEAVIAKEDLREACLNYLINIKRLLLCPFSNRNFSSLDLYSNLIQHLLSANSSPRKSRLSFGGRPGSPSKISLSLNRFYNQGGLSKSCATLPSQMYNLDHNNISQKSLKFNTHNTSKASAEKTVEHLEAFKGGFKYHTDLKNLLYPLSEKEKIEGDELYDNILKETFNEELLSHYPPNIIYATFQKYLSSFINRKFGVLLSSSFIQQLNTVENLNLSFNSTDAVYHLKKILQDLPESSLKILENIFSIASDLLLRLPLKDQCDFVTKQLAIALAPSMFGSNAVELVYYLAYHSDRIFGTVEELPTPVSPANSNNDKQLDESKFQAIAMKEMPERHPKEILPGQIEREAYEDLRRKYHLTLARLAQMTRLNEDSKKSIPLLYDRFNHDLKLIKQSVQASLIRKQCELDTAKWTLEEYESKLNAKEGCQTNIFI B26C47589F76F50B klp8-blt1-1 2 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 10 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association cdc15-blt1-1 5 12 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 8 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association cdr2-mid1-1 1 11 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 9 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association cdc15-blt1-2 7 12 unspecified role unspecified role bait bait YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association blt1-gef2-1 3 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 13 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association cdr2-blt1-1 6 8 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 9 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association blt1-mid1-1 4 11 unspecified role unspecified role prey prey schpm Schizosaccharomyces pombe (fission yeast) Fission yeast 8 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range schpm Schizosaccharomyces pombe (fission yeast) Fission yeast physical association physical association