DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. PLoS biology 2009 imex curation Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. DIP original experiment: DIP-132630X PLoS biology 2009 IMEx Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. PLoS biology 2009 imex curation Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting anti tag western anti tag western blot anti tag western Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. DIP original experiment: DIP-132627X PLoS biology 2009 IMEx Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. PLoS biology 2009 imex curation Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. DIP original experiment: DIP-132629X PLoS biology 2009 IMEx Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. PLoS biology 2009 imex curation Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Burkard ME., Maciejowski J., Rodriguez-Bravo V., Repka M., Lowery DM., Clauser KR., Zhang C., Shokat KM., Carr SA., Yaffe MB., Jallepalli PV. DIP original experiment: DIP-132628X PLoS biology 2009 IMEx Plk1 self-organization and priming phosphorylation of HsCYK-4 at the spindle midzone regulate the onset of division in human cells. plk1_human Serine/threonine-protein kinase PLK1 Polo-like kinase 1 Serine/threonine-protein kinase 13 PLK1 PLK protein protein human Homo sapiens Human MSAAVTAGKLARAPADPGKAGVPGVAAPGAPAAAPPAKEIPEVLVDPRSRRRYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQREKMSMEISIHRSLAHQHVVGFHGFFEDNDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLHRNRVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYDGERKKTLCGTPNYIAPEVLSKKGHSFEVDVWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTINELLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSNRKPLTVLNKGLENPLPERPREKEEPVVRETGEVVDCHLSDMLQQLHSVNASKPSERGLVRQEEAEDPACIPIFWVSKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKITLLKYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGSVQINFFQDHTKLILCPLMAAVTYIDEKRDFRTYRLSLLEEYGCCKELASRLRYARTMVDKLLSSRSASNRLKAS DIP protein P53350 original sequence version: 169 DIP protein P53350 original sequence version: 170 DIP protein P53350 original sequence version: 164 DIP protein P53350 original sequence version: 154 DIP protein P53350 original sequence version: 91 DIP protein P53350 original sequence version: 145 DIP protein P53350 original sequence version: 121 DIP protein P53350 original sequence version: 118 DIP protein P53350 original sequence version: 123 DIP protein P53350 original sequence version: 119 DIP protein P53350 original sequence version: 138 DIP protein P53350 original sequence version: 163 DIP protein P53350 original sequence version: 178 178C2F13C10E8206 rgap1_human Rac GTPase-activating protein 1 Male germ cell RacGap RACGAP1 KIAA1478 MGCRACGAP Protein CYK4 homolog protein protein human Homo sapiens Human MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLSEEQKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIETVPYWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK DIP protein Q9H0H5 original sequence version: 87 DIP protein Q9H0H5 original sequence version: 144 032B7DF9CEA8F39D ect2_human Protein ECT2 Epithelial cell-transforming sequence 2 oncogene ECT2 protein protein human Homo sapiens Human MAENSVLTSTTGRTSLADSSIFDSKVTEISKENLLIGSTSYVEEEMPQIETRVILVQEAGKQEELIKALKTIKIMEVPVIKIKESCPGKSDEKLIKSVINMDIKVGFVKMESVEEFEGLDSPEFENVFVVTDFQDSVFNDLYKADCRVIGPPVVLNCSQKGEPLPFSCRPLYCTSMMNLVLCFTGFRKKEELVRLVTLVHHMGGVIRKDFNSKVTHLVANCTQGEKFRVAVSLGTPIMKPEWIYKAWERRNEQDFYAAVDDFRNEFKVPPFQDCILSFLGFSDEEKTNMEEMTEMQGGKYLPLGDERCTHLVVEENIVKDLPFEPSKKLYVVKQEWFWGSIQMDARAGETMYLYEKANTPELKKSVSMLSLNTPNSNRKRRRLKETLAQLSRETDVSPFPPRKRPSAEHSLSIGSLLDISNTPESSINYGDTPKSCTKSSKSSTPVPSKQSARWQVAKELYQTESNYVNILATIIQLFQVPLEEEGQRGGPILAPEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFFEMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKHTADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCPANLLSSHRSLVQRVETISLGEHPCDRGEQVTLFLFNDCLEIARKRHKVIGTFRSPHGQTRPPASLKHIHLMPLSQIKKVLDIRETEDCHNAFALLVRPPTEQANVLLSFQMTSDELPKENWLKMLCRHVANTICKADAENLIYTADPESFEVNTKDMDSTLSRASRAIKKTSKKVTRAFSFSKTPKRALRRALMTSHGSVEGRSPSSNDKHVMSRLSSTSSLAGIPSPSLVSLPSFFERRSHTLSRSTTHLI DIP protein Q9H8V3 original sequence version: 73 DIP protein Q9H8V3 original sequence version: 72 DIP protein Q9H8V3-1 original sequence version: 72 DIP protein Q9H8V3 original sequence version: 130 F40FC4F982FB8C78 plk1-racgap1-2 2 6 unspecified role unspecified role prey prey CBD-tag CBD-tag fusion protein fusion protein n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 1-288 Region 1-288 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9H0H5:p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala] S157A/S164A/S170A/S214A/S260A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 19:19:55 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser157ala,ser164ala,ser170ala,ser214ala,thr260ala' Tue Jun 30 18:20:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala]' ecolx Escherichia coli 5 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 345-603 Region 345-603 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction plk1-racgap1-3 3 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 1-288 Region 1-288 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9H0H5:p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala] S157A/S164A/S170A/S214A/S260A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:05:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser157ala,ser164ala,ser170ala,ser214ala,thr260ala' Tue Jun 30 17:00:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala]' ecolx Escherichia coli 5 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation racgap1-ect2-2 4 5 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait human Homo sapiens Human 7 unspecified role unspecified role prey prey human Homo sapiens Human association association ect2-racgap1-11 1 6 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9H0H5:p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala] S157A/S164A/S170A/S214A/S260A mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 16:22:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser157ala,ser164ala,ser170ala,ser214ala,thr260ala' Tue Jun 30 17:47:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser157Ala;Ser164Ala;Ser170Ala;Ser214Ala;Thr260Ala]' Q9H0H5:p.Ser157Ala S157A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S157A'. Wed May 02 18:28:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser157ala' Tue Jun 30 19:36:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser157Ala' Q9H0H5:p.[Ser157Ala;Ser164Ala;Ser170Ala] S157A/S164A/S170A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A This feature has been corrected as a result of our quality control procedures. The original label was 'S157A/S164A/S170A'. Wed May 02 20:15:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser157ala,ser164ala,ser170ala' Tue Jun 30 17:30:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser157Ala;Ser164Ala;Ser170Ala]' human Homo sapiens Human 7 unspecified role unspecified role bait bait Region 1-352 Region 1-352 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association