DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 imex curation Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249904X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 imex curation Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249906X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 imex curation Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249905X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 imex curation Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249902X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. Nature 2009 imex curation Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Wang GG., Song J., Wang Z., Dormann HL., Casadio F., Li H., Luo JL., Patel DJ., Allis CD. DIP original experiment: DIP-249903X Nature 2009 IMEx Haematopoietic malignancies caused by dysregulation of a chromatin-binding PHD finger. h3tail H3 tail peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ARTKQTARKSTGGKAPRKQL kdm5a_human Lysine-specific demethylase 5A Jumonji/ARID domain-containing protein 1A Retinoblastoma-binding protein 2 KDM5A RBBP2 RBP2 JARID1A Histone demethylase JARID1A [histone H3]-trimethyl-L-lysine(4) demethylase 5A protein protein human Homo sapiens Human MAGVGPGGYAAEFVPPPECPVFEPSWEEFTDPLSFIGRIRPLAEKTGICKIRPPKDWQPPFACEVKSFRFTPRVQRLNELEAMTRVRLDFLDQLAKFWELQGSTLKIPVVERKILDLYALSKIVASKGGFEMVTKEKKWSKVGSRLGYLPGKGTGSLLKSHYERILYPYELFQSGVSLMGVQMPNLDLKEKVEPEVLSTDTQTSPEPGTRMNILPKRTRRVKTQSESGDVSRNTELKKLQIFGAGPKVVGLAMGTKDKEDEVTRRRKVTNRSDAFNMQMRQRKGTLSVNFVDLYVCMFCGRGNNEDKLLLCDGCDDSYHTFCLIPPLPDVPKGDWRCPKCVAEECSKPREAFGFEQAVREYTLQSFGEMADNFKSDYFNMPVHMVPTELVEKEFWRLVSSIEEDVIVEYGADISSKDFGSGFPVKDGRRKILPEEEEYALSGWNLNNMPVLEQSVLAHINVDISGMKVPWLYVGMCFSSFCWHIEDHWSYSINYLHWGEPKTWYGVPSHAAEQLEEVMRELAPELFESQPDLLHQLVTIMNPNVLMEHGVPVYRTNQCAGEFVVTFPRAYHSGFNQGYNFAEAVNFCTADWLPIGRQCVNHYRRLRRHCVFSHEELIFKMAADPECLDVGLAAMVCKELTLMTEEETRLRESVVQMGVLMSEEEVFELVPDDERQCSACRTTCFLSALTCSCNPERLVCLYHPTDLCPCPMQKKCLRYRYPLEDLPSLLYGVKVRAQSYDTWVSRVTEALSANFNHKKDLIELRVMLEDAEDRKYPENDLFRKLRDAVKEAETCASVAQLLLSKKQKHRQSPDSGRTRTKLTVEELKAFVQQLFSLPCVISQARQVKNLLDDVEEFHERAQEAMMDETPDSSKLQMLIDMGSSLYVELPELPRLKQELQQARWLDEVRLTLSDPQQVTLDVMKKLIDSGVGLAPHHAVEKAMAELQELLTVSERWEEKAKVCLQARPRHSVASLESIVNEAKNIPAFLPNVLSLKEALQKAREWTAKVEAIQSGSNYAYLEQLESLSAKGRPIPVRLEALPQVESQVAAARAWRERTGRTFLKKNSSHTLLQVLSPRTDIGVYGSGKNRRKKVKELIEKEKEKDLDLEPLSDLEEGLEETRDTAMVVAVFKEREQKEIEAMHSLRAANLAKMTMVDRIEEVKFCICRKTASGFMLQCELCKDWFHNSCVPLPKSSSQKKGSSWQAKEVKFLCPLCMRSRRPRLETILSLLVSLQKLPVRLPEGEALQCLTERAMSWQDRARQALATDELSSALAKLSVLSQRMVEQAAREKTEKIISAELQKAAANPDLQGHLPSFQQSAFNRVVSSVSSSPRQTMDYDDEETDSDEDIRETYGYDMKDTASVKSSSSLEPNLFCDEEIPIKSEEVVTHMWTAPSFCAEHAYSSASKSCSQGSSTPRKQPRKSPLVPRSLEPPVLELSPGAKAQLEELMMVGDLLEVSLDETQHIWRILQATHPPSEDRFLHIMEDDSMEEKPLKVKGKDSSEKKRKRKLEKVEQLFGEGKQKSKELKKMDKPRKKKLKLGADKSKELNKLAKKLAKEEERKKKKEKAAAAKVELVKESTEKKREKKVLDIPSKYDWSGAEESDDENAVCAAQNCQRPCKDKVDWVQCDGGCDEWFHQVCVGVSPEMAENEDYICINCAKKQGPVSPGPAPPPSFIMSYKLPMEDLKETS DIP protein P29375 original sequence version: 85 DIP protein P29375-1 original sequence version: 91 DIP protein P29375 original sequence version: 130 DIP protein P29375-1 original sequence version: 116 FCF6DC22DEF001DF h3peptide-kdm5a-1 1 6 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3peptide-kdm5a-2 4 6 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3peptide-kdm5a-3 2 6 unspecified role unspecified role bait bait K4me2 K4me2 n6me2lys N6,N6-dimethyl-L-lysine (2S)-2-amino-6-(dimethylamino)hexanoic acid MOD_RES N6,N6-dimethyllysine N(zeta)-dimethyllysine N6,N6-dimethyl-L-lysine N6,N6-dimethylated L-lysine epsilon-dimethyllysine lysine derivative Lys(y) Dimethyl N6Me2Lys di-Methylation dimethylk certain certain sequence position certain certain certain sequence position certain K4me3 K4me3 n6me3+lys N6,N6,N6-trimethyl-L-lysine (5S)-5-amino-5-carboxy-N,N,N-trimethylpentan-1-aminium 2-amino-6-(trimethylammonio)hexanoic acid 5-azanyl-5-carboxy-N,N,N-trimethylpentanazanium MOD_RES N6,N6,N6-trimethyllysine N(zeta)-trimethyllysine N6,N6,N6-trimethyl-L-lysine N6,N6,N6-trimethylated L-lysine N6,N6,N6-trimethyllysin-N6-ium N6,N6,N6-trimethyllysine cation epsilon-trimethyllysine N-trimethylation (of lysine) N6Me3+Lys Trimethyl tri-Methylation certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 7 unspecified role unspecified role prey prey Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P29375:p.Trp1625Ala W1625A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A This feature has been corrected as a result of our quality control procedures. The original label was 'W1625A'. Wed May 02 16:12:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp1625ala' Tue Jun 30 17:08:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp1625Ala' P29375:p.[Glu1634Ala;Trp1635Ala] E1634A/W1635A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain E A This feature has been corrected as a result of our quality control procedures. The original label was 'E1634A/W1635A'. Wed May 02 19:39:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu1634ala,trp1635ala' Tue Jun 30 16:39:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu1634Ala;Trp1635Ala]' ecolx Escherichia coli direct interaction direct interaction h3peptide-kdm5a-4 3 6 unspecified role unspecified role unspecified role unspecified role Region 1-15 Region 1-15 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction h3peptide-kdm5a-5 5 6 unspecified role unspecified role unspecified role unspecified role Region 1-9 Region 1-9 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 7 unspecified role unspecified role unspecified role unspecified role Region 1601-1660 Region 1601-1660 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction