DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. Structure (London, England : 1993) 2009 imex curation Warmuth S., Zimmermann I., Dutzler R. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Warmuth S., Zimmermann I., Dutzler R. DIP original experiment: DIP-132090X Structure (London, England : 1993) 2009 IMEx X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. Structure (London, England : 1993) 2009 imex curation Warmuth S., Zimmermann I., Dutzler R. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Warmuth S., Zimmermann I., Dutzler R. DIP original experiment: DIP-132088X Structure (London, England : 1993) 2009 IMEx X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. Structure (London, England : 1993) 2009 imex curation Warmuth S., Zimmermann I., Dutzler R. Only protein-protein interactions ecolx Escherichia coli solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Warmuth S., Zimmermann I., Dutzler R. DIP original experiment: DIP-132089X Structure (London, England : 1993) 2009 IMEx X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. Structure (London, England : 1993) 2009 imex curation Warmuth S., Zimmermann I., Dutzler R. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Warmuth S., Zimmermann I., Dutzler R. DIP original experiment: DIP-132091X Structure (London, England : 1993) 2009 IMEx X-ray structure of the C-terminal domain of a prokaryotic cation-chloride cotransporter. q8thk8_metac MA_4506 protein protein metac Methanosarcina acetivorans (strain ATCC 35395 / DSM 2834 / JCM 12185 / C2A) MGGQKNLENEPFEAGEGEPLEAGEDTKSLQEQVEEQKAKPVLFGTFEGVFVPNLLTILGVIMYLREGWVVGNAGLLGAWLIILLSFAITTCTGLSLSSITTNIRIGAGGAFSIISQSLGLEVGGSIGIPLYLSQALAVSMYIFGIRAGWRWFFPEHPALYIDLAAFILLFVIAYISARLAFRIQYFILVVIVASLVSVFWTGFGDSMQGSVQLWGSFPGSPETGFVGIGFWEVFAVYFPAATGIMAGANMSGELKTPRKSIPLGTLSVIGISLCIYLALAYWFALSATPEELVSNYTIIFEKAAFGPIVVAGLLGATFSSALNSIVGAPRILQALGEHGILPKSEWFSQKTDRGEPRNAILFTGTIVLGAIMLRNLNSVAPLITMFFLITYSMINVVVFIEQNLKLVSFRPLFKIPLSVSFLGAAGSLFAMFIINPGFSLLAVVVVLLIHNYLLRKHLKAPFGDVRSGLFVTVAEWAAKKTNSLTSSSERTWKANLLVPVEDPRELMGTFDFLRDITYPKGSVKLLGLAGNTDKENLLSQLPSISEGFQEEGVFSSWTIIDTAEFEENLVVGMEALTGSFFRPSILFLRLPENRDRDEEIREIIRKASMYRMGVLLFSKHPQAGLGRQNLINLWIENRGLDWDISMELGNMDLALLIAYKLKSNWKASLSFMTFAPTAIQAQAAENFLQSLAELARIPNVKMQVLRENPIKSSKLPFASLHIFSLDPNPDLDLARHLMEKAGSSCIFALDSGEENALA B963B92868732C84 maccc-1 4 5 unspecified role unspecified role unspecified role unspecified role Region 474-758 Region 474-758 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Q8THK8:p.[Glu505Cys;Gln622Cys] E505C/Q622C mutation mutation certain certain sequence position certain certain certain sequence position certain Q C certain certain sequence position certain certain certain sequence position certain E C This feature has been corrected as a result of our quality control procedures. The original label was 'E505C/Q622C'. Wed May 02 15:41:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu505cys,gln622cys' Tue Jun 30 17:09:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu505Cys;Gln622Cys]' ecolx Escherichia coli direct interaction direct interaction maccc-2 2 5 unspecified role unspecified role unspecified role unspecified role Region 474-758 Region 474-758 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction maccc-3 3 5 unspecified role unspecified role unspecified role unspecified role Region 474-758 Region 474-758 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8THK8:p.Arg627Ala R627A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'R627A'. Wed May 02 19:32:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg627ala' Tue Jun 30 18:14:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg627Ala' ecolx Escherichia coli direct interaction direct interaction maccc-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 474-758 Region 474-758 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction