DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133843X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133835X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133844X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133841X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133842X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133837X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133839X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133840X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133838X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. Only protein-protein interactions maize Zea mays (Maize) Maize anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Kroeger TS., Watkins KP., Friso G., van Wijk KJ., Barkan A. DIP original experiment: DIP-133836X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing. caf2p_maize CRS2-associated factor 2, chloroplastic CAF2 Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 2 protein protein maize Zea mays (Maize) Maize MPPPPPQRPASSHVGRANLFSASPPPLSNRRYPHHRSLPLPPVSPRRRDPKKHSQQPSQEEPTDSGPTRTVTTNPAFRAAHLRTAYRKPVPPAAAAGEGEALLAADPTDAASGRAVVVGPSGLSFRLPGAPFDFQFSYSEAPRAPPLAIREPAFLPFAPPTMPRPWTGKAPLLTKEEKARRRGVRLHTPLGQETPQTVSAHGIMMEVRERRKMDLARVSPGDGRSREEVLGEPLTPSEVRALVKPHISHNRQLNIGRDGLTHNMLEMIHCHWRRQEICKVRCRGVPTVDMKNLCYHLEEKSGGKVIHRVGGVVFLYRGRHYDPKTRPRYPLMLWKPATPVYPKLIKEAPDGFTKEEADEMRRKGRDLLPICKLAKNGIYITLVKDVRDAFEGSDLVKIDCEGLNPSDYKKIGAKLRDLVPCVLLSFDDEQILMHRGKEWKSRYSKPLTLIPKVPKNNLAMTSVMNSSDEVSDANTQVAIREVLRPKMFKLWKSAVDSSLALLLDDAEANNLTPDSLLTLVEEFSVTSQAVEHSFPALLVTNGDASTDSLSAEYMNDEPETSVAGNEEGQLEQSPDLRDDEHFDVDMFERLESSVPLGSLPIDSMIERLNSE DIP protein Q84N48 original sequence version: 27 rnc1_maize Ribonuclease III domain-containing protein RNC1, chloroplastic RNC1 Zm.27802 Chloroplast ribonuclease III domain protein protein protein maize Zea mays (Maize) Maize MGPPAMAFQALTLTPLPFSLHSSSRRVRVLAVAADQTPPPAPPSEPANSPSRLLRELAQRKKAVSPKKKHPPRRFILKPPLDDERLTRRFLSSPQLSLKALPLLSSCLPSAPLSTADRTWMDEYLLEAKQALGYPLAPSETLGEGDDCPARHFDVLFYLAFQHLDPSSERTRMRHVRNGHSRLWFLGQYVLELAFCEFFLQRYPRESPGPMRERVFALIGKKVLPRWLKAASLHNLVFPYDDLDKMIRKDREPPSKAVFWAIFGAIYLCFGMPEVYRVLFEAFGMDPDDESCQPKLRRQLEDVDYVSVEFEKRQLTWQDVAAYRPPPDALFAHPRLFRACVPPGMHRFRGNIWDFDSRPKVMTTLGYPLPMNDRIPEITEARNIELGLGLQLCFLHPSKHKFEHPRFCYERLEYVGQKIQDLVMAERLLMKHLDAPGRWLAEKHRRTLMNKYCGRYLRDKHLQHYIIYGETVQDRFEHNRRLRNPSTTSVQQALHGLAYCVYGKPDVRRLMFEVFDFEQVQPKAV DIP protein A6YSL1 original sequence version: 17 cfm3_maize CRM-domain containing factor CFM3, chloroplastic/mitochondrial CFM3 Protein CRM FAMILY MEMBER 3 protein protein maize Zea mays (Maize) Maize MAMASSPACHFRHPPRLRLLLPLSTSAPHPWLYSWSHPRQRGRLRAPPAALDLRPEPSPSSDSDDEDAVGASRSSGRSTMSLILSRLRRAGYSGEDPRAAAPPHPPRGSVEDVFRADDGVLPNARGGFDADDEERALGDARFPWERPMPPPEAAPRSARSPTWMAELTLPAAELRRLRHAAIRIKSRTKVGGAGVTREIVEKIKEKWKTEEVVRVKVSGTPALNMRLFHEILERKTGGLVIWRSGTSVSLYRGVDYDEPEPTKKSKKNSQSLAMDFPIKGSSNPSLLPTETANSVRDSNVALVSNAAKEELVVQAPEIKYEDEIDKLLDELGPRYTDWPGSDPLPVDADLLPANMPGYKPPFRVLPYGVRPSLSRRDTTNLRRLARGLPPHFALGRSRQLQGLANAMVKLWEKSSIAKIALKRGVQLTTSERMAEDIKKLTGGVMLSRNNEFIVFYRGKDFLSSELAEVLLERERLAKSLQDEEEARRKAASYFSSAETYAQPTVAGTLGETLEANSKYGTKHDENHADKMARTIEAARHADLVRKLEWKLSLAQKKMEKAERVLGKVETALRPTEDSRPPETITDEERFMFRKLGLRMKAFLLLGRRGVFDGTIENMHLHWKYRELVKILVKAKSFADVKRIALSLEAESGGILVSVDKVSKGYAIVVFRGKNYRRPSSLRPRNLLSKRKALARSIELQRHQALSRHFAKLNRKVERLKAELVQMEDVKEQGDEELYAKLDAAYSSDDEDMEDEDDEAYLKRFDNEVAGATADDDGSDDYTSAADEADYPDSDDEAGDCSEDEGEDDEDEAAAGVSDAGFHGEVVGFSSDTDRRNHDVNEY DIP protein A7XN92 original sequence version: 10 a7udm2_maize CFM2 protein protein maize Zea mays (Maize) Maize VGSTLDPSWATGDGEASSTSRQRRRGRGRDASGSPSAPPSAAELALPRDELRRLQGIGIRVRKRLKVGKAGITEGIVNGIHERWRNAEVVKLRCEDVWAMNMRRTHEILERKTGGLVIWRSGSTIILYRGTNYTYPYFHHSERVDSFLDKESSDQSNSGDEDETSSQHGSSHEKSSENPVVACAEQIHVGEGNSQTIEYLNQSLSREKDTNHPVSSIKRLVFDADEGNLDIRAGNPNEQHVRLQENTHPDSPNKFGPRDRSSLVAGVGSQNKFRLQLPGEVKLAEEADKLLDGLGPRFSGWWGYDPVPVDADLLPAIVPGYRRPFRLLPSGVPPKLTDREMTILRRLAHALPFHYALGRSSNLQGLAASMIKLWERCEVAKIALKRDAHNTDSELITEEVKELTGGTLLSRDKESIVFYRGKDFLPPAVSLAIEKRRKLGSSTIYKAKPGIEESMPTQNDSVLKVSSDVSVHVREEGTSVTENRAESLNTVAKDVETRLSQAIAEKAKAEKLIEELEKASPLSKAEVRETISEDERYMLRKVGLKMKQFLLLGRRGVFDGTIENMHLHWKYRELVKIICKEHRLEDVEYAARTLEAESGGILVAVEKVSKGHAIIVYRGKNYKRPSKLRPKTLLSKRDALKRSLENQRCKSLKVHVLKLSKNIDYLRDQMNSSYYHKDMHDPSVNSVTLQQQDEEMPEVAPMSSEPEVEKWTSVEIDRALDLTKSGVPVEDMQSKVCFNKLEDDSSATAGPCLTGSSIAASSYNLIRHQNQRSSTVTSSPDGRYEGAPSKVVDAPKLDAESLSVSPLRAAPLSNQERLVLRKQALQMKKRPVLSIGRNNAITGVAKTIKTHFKKHPLAIVNIKNRADGTPIQQLISELEEATGSVLVSRETNKVILYRGWGAEVAQKSSRESSTDEGEKEVISPQLLEAIRLECGLLPADSG DIP protein A7UDM2 original sequence version: 9 wtf1_maize Protein WHAT'S THIS FACTOR 1, chloroplastic WTF1 ZEAMMB73_Zm00001d009618 protein protein maize Zea mays (Maize) Maize MDAKLLLPFPFAPAAATRSPKSLFLGAPLPPPPRPPPFPLRLRPRPAAVVAQAAVKRRKEAPFDTVIQRDKKLKLVLKLRNILVAQPDRVMSLRELGRFRRDLGLTRKRRLIALLRRFPGVFDVVEEGVYSLRFRLTPAAERLYLDELRLRNESEGLAVAKLRKLLMMSQEKRILIEKVAHLKHDLGLPPEFRDTVCLRYPQYFRVVRMDRGPALELTHWDPELAVSAAELAEEESRAREAEERNLIIDRPLKFNRVRLPKGLKLTRGEARRIARFKEMPYISPYADFSHLRSGSDEKEKHACGVVHEILSLTVEKRTLVDHLTHFREEFRFSQSLRGMIIRHPDMFYVSFKGDRDSVFLREAYKDSQLVEKNQLVLLKEKMRALVAVPRFPRRAAVGTGEEAEGMNGSLQSRDQVSDEEYDDEDEGLSDMEDLISELSGGKSDADYEWGDGWFGENDDSPPDFGDDEVKVAMKIADGSANGSAPVPVFPDGRPRERW DIP protein B6TTV8 original sequence version: 9 caf1p_maize CRS2-associated factor 1, chloroplastic CAF1 Chloroplastic group IIA intron splicing facilitator CRS2-associated factor 1 protein protein maize Zea mays (Maize) Maize MATARLPSRSFLSPAQQSYPRLPASVRLCLSHHEQPPTGPKRHRRAATSHPAFSAAARGRAKKIPIADTDEPAAGVRVTDRGISYRLDGAPFEFQYSYTEAPRARPVALREAPFMPFGPEATPRPWTGRKPLPKSRKELPEFDSFVLPAPGKKGVKPVQSPGPFLAGMEPRYQSVSREDILGEPLTKEEVSELVKGSLKSKRQLNMGRDGLTHNMLENIHSHWKRKRVCKIKCKGVCTIDMDNICHQLEEKVGGKVIHRQGGVIFLFRGRNYNYRTRPCFPLMLWKPVAPVYPRLVTKVPGGLTPDEATEMRTRGHQLPPICKLGKNGVYANLVNQVREAFEACDLVRVDCSGLNKSDCRKIGAKLKDLVPCILLSFEFEHILMWRGSDWKSSLPPLENSYEVTKVQESFSGKESNEKVTHSGNVLAQIELVSAATSHKNWNLDEGQEKFKDSTDSDMVLNSAKDVPALFHSTGISRTEPSADIPLEYSPLNPVCDIMDPSLNCRSIPTNNCESRALVEKSEHCPDDYNLEMKRKRNDGTKGTVVLNSGSKVEGLLCLLEQAIHSGRALVLSEDELADSDLVYEKSVAFTKSIPRRLVFENTRRKSSARRNVPDNHARTKTHLVENKMLSSPVENKFIVNGGSAMQTIDHGQEFLSDVVHQGTLRVDELAKLLA DIP protein Q84N49 original sequence version: 30 crs1_maize Chloroplastic group IIA intron splicing facilitator CRS1, chloroplastic CRS1 Chloroplastic RNA splicing factor 1 Protein CHLOROPLAST RNA SPLICING 1 protein protein maize Zea mays (Maize) Maize MAPPPLPLFSPSLKAPPPPPWLHGSSTQSRDSAPPVPPLPAEATPSKFRIDSPKPAPARKNTKTAAKPLTAGVPGGRTHRAVLGIIRRVRSLELSDAPSPNSVHTSNSGAAAAAFHLTIELSPPREPGQYVVEKEKSRAVPWAAARDEGLKVALRREKKPREPTRAETELETHELRRLRRLARGIGRWARAKKAGVTDEVVKEVRREWASGEELAAVRIVEPLRRSMDRAREILEIKTGGLVVWTKGDMHFVYRGSKYQQNAKHSHTFLTNVHKDDAFQENDQSICGQKDEEPVKGTLYEREVNRLLDTLGPRFVDWWWDTPLPVDADLLPEFVPGSKTPYRLCPPGVRPTLADEELTYLRKLARLLPTHFALGRNTRLQGLAAAILKLWEKSLIAKIAVKIGIQNTNNEQMAWNLKHLTGGTVILRNKDFIILYRGKDFLPGGVAQTVIQREAQVHDEQVKEEEARLKAVDSLQMVGELSEESSLGTFREYQGFHAKFVHENTENSNTMIELEAEKYRLEKELKDHEWKLSVLNKKIERSNQALAKLHSSWSPSEQSADREHLTEEEKIMFRRIGRKMDGLVLLGRRGIFDGVIEEIHQHWKHKEVVKVITKQNQTRQIMYAASLLEVETGGILIAVEKLTTSHAIILYRGKNYRRPAKSSFSNLLTKREALRRSIEVQRRGSMKYFVRERQKSILELKRKLRYVTRQIRYRTP DIP protein Q9FYT6 original sequence version: 31 caf1-b6ttv8_maize-1 2 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize physical association physical association cfm3-b6ttv8_maize-1 5 12 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 13 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize association association caf2-b6ttv8_maize-1 7 13 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 14 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 12 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize association association cfm2-caf2-1 4 11 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 14 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize physical association physical association rnc1-b6ttv8_maize-1 8 13 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 12 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize association association b6ttv8_maize-rnc1-1 1 15 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 12 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction caf2-b6ttv8_maize-2 10 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize physical association physical association caf1-b6ttv8_maize-2 9 13 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 12 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 15 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize association association b6ttv8_maize-caf1-1 6 13 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 17 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 12 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 11 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 16 unspecified role unspecified role prey prey maize Zea mays (Maize) Maize 15 unspecified role unspecified role bait bait maize Zea mays (Maize) Maize association association b6ttv8_maize-rnc1-2 3 15 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction