DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133802X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133800X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip ms participant Identification by mass spectrometry ms participant Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133796X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133801X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay tag visualisation tag visualisation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133798X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro filter binding filter binding Filter overlay assay Filter overlay assay predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133799X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Hatakeyama S., Sugihara K., Nakayama J., Akama TO., Wong SM., Kawashima H., Zhang J., Smith DF., Ohyama C., Fukuda M., Fukuda MN. DIP original experiment: DIP-133797X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx Identification of mRNA splicing factors as the endothelial receptor for carbohydrate-dependent lung colonization of cancer cells. srsf2_mouse Serine/arginine-rich splicing factor 2 Sfrs2 Splicing factor, arginine/serine-rich 2 Srsf2 Pr264 Sfrs10 Splicing factor SC35 Splicing component, 35 kDa Protein PR264 Putative myelin regulatory factor 1 protein protein mouse Mus musculus Mouse MSYGRPPPDVEGMTSLKVDNLTYRTSPDTLRRVFEKYGRVGDVYIPRDRYTKESRGFAFVRFHDKRDAEDAMDAMDGAVLDGRELRVQMARYGRPPDSHHSRRGPPPRRYGGGGYGRRSRSPRRRRRSRSRSRSRSRSRSRSRYSRSKSRSRTRSRSRSTSKSRSARRSKSKSSSVSRSRSRSRSRSRSRSPPPVSKRESKSRSRSKSPPKSPEEEGAVSS DIP protein Q62093 original sequence version: 89 DIP protein Q62093 original sequence version: 102 68121AC4D35714FA srsf7_mouse Serine/arginine-rich splicing factor 7 Srsf7 Sfrs7 Splicing factor, arginine/serine-rich 7 protein protein mouse Mus musculus Mouse MRSSARGRPLQAATAFFLSLFFFLRRFERGFWLWGGDSETKVYVGNLGTGAGKGELERAFSYYGPLRTVWIARNPPGFAFVEFEDPRDAEDAVRGLDGKVICGSRVRVELSTGMPRRSRFDRPPARRPFDPNDRCYECGEKGHYAYDCHRYSRRRRSRSRSRSHSRSRGRRYSRSRSRSRGRRSRSASPRRSRSVSLRRSRSASLRRSRSGSIIGSRYFQSRSRSRSRSRSISRPRSSRSKSRSPSPKRSRSPSGSPHRSASPERMD DIP protein Q8BL97 original sequence version: 69 F193FC4C3A7D8C7C srsf1_mouse Serine/arginine-rich splicing factor 1 Sfrs1 Splicing factor, arginine/serine-rich 1 Srsf1 ASF/SF2 Pre-mRNA-splicing factor SRp30a protein protein mouse Mus musculus Mouse MSGGGVIRGPAGNNDCRIYVGNLPPDIRTKDIEDVFYKYGAIRDIDLKNRRGGPPFAFVEFEDPRDAEDAVYGRDGYDYDGYRLRVEFPRSGRGTGRGGGGGGGGGAPRGRYGPPSRRSENRVVVSGLPPSGSWQDLKDHMREAGDVCYADVYRDGTGVVEFVRKEDMTYAVRKLDNTKFRSHEGETAYIRVKVDGPRSPSYGRSRSRSRSRSRSRSRSNSRSRSYSPRRSRGSPRYSPRHSRSRSRT DIP protein Q6PDM2 original sequence version: 80 C28A0B2F112EA713 anxa1_human Annexin A1 ANXA1 ANX1 LPC1 Annexin-1 Annexin I Lipocortin I Calpactin II Chromobindin-9 p35 Phospholipase A2 inhibitory protein Calpactin-2 protein protein human Homo sapiens Human MAMVSEFLKQAWFIENEEQEYVQTVKSSKGGPGSAVSPYPTFNPSSDVAALHKAIMVKGVDEATIIDILTKRNNAQRQQIKAAYLQETGKPLDETLKKALTGHLEEVVLALLKTPAQFDADELRAAMKGLGTDEDTLIEILASRTNKEIRDINRVYREELKRDLAKDITSDTSGDFRNALLSLAKGDRSEDFGVNEDLADSDARALYEAGERRKGTDVNVFNTILTTRSYPQLRRVFQKYTKYSKHDMNKVLDLELKGDIEKCLTAIVKCATSKPAFFAEKLHQAMKGVGTRHKALIRIMVSRSEIDMNDIKAFYQKMYGISLCQAILDETKGDYEKILVALCGGN DIP protein P04083 original sequence version: 146 14B42E1FA4178EC0 ipeptide I-peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) IELLQARC srsf5_mouse Serine/arginine-rich splicing factor 5 Srsf5 Hrs Sfrs5 Delayed-early protein HRS Pre-mRNA-splicing factor SRP40 Splicing factor, arginine/serine-rich 5 protein protein mouse Mus musculus Mouse MSGCRVFIGRLNPAAREKDVERFFKGYGRIRDIDLKRGFGFVEFEDPRDADDAVYELDGKELCSERVTIEHARARSRGGRGRGRYSDRFSSRRPRNDRRNAPPVRTENRLIVENLSSRVSWQDLKDFMRQAGEVTFADAHRPKLNEGVVEFASYGDLKNAIEKLSGKEINGRKIKLIEGSKRHRSRSRSRSRTRSSSRSRSRSRSRRSKSYSRSRSRSRSRSKSRSGSRSPVPEKSQKRGSSSRSKSPASVDRQRSRSRSRSRSVDSGN DIP protein O35326 original sequence version: 80 srsf5-ipeptide-1 2 13 unspecified role unspecified role bait bait ecolx Escherichia coli 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction ipeptide-srsf1-1 3 9 unspecified role unspecified role prey prey mouse Mus musculus Mouse 13 unspecified role unspecified role prey prey mouse Mus musculus Mouse 8 unspecified role unspecified role prey prey mouse Mus musculus Mouse 10 unspecified role unspecified role prey prey mouse Mus musculus Mouse 12 unspecified role unspecified role bait bait in vitro In vitro association association anxa1-ipeptide-1 1 11 unspecified role unspecified role bait bait ecolx Escherichia coli 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction srsf1-ipeptide-1 5 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction srsf2-ipeptide-1 6 8 unspecified role unspecified role bait bait ecolx Escherichia coli 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction srsf7-ipeptide-1 4 9 unspecified role unspecified role bait bait ecolx Escherichia coli 12 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro direct interaction direct interaction ipeptide-srsf1-2 7 12 unspecified role unspecified role unspecified role unspecified role A488-label A488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction