DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133738X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay western blot western blot Immuno blot Immuno blot Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133742X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133743X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133741X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133744X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133739X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Mintz PJ., Cardó-Vila M., Ozawa MG., Hajitou A., Rangel R., Guzman-Rojas L., Christianson DR., Arap MA., Giordano RJ., Souza GR., Easley J., Salameh A., Oliviero S., Brentani RR., Koivunen E., Arap W., Pasqualini R. DIP original experiment: DIP-133740X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx An unrecognized extracellular function for an intracellular adapter protein released from the cytoplasm into the tumor microenvironment. nstpeptide PSI domain peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) NSTFLQEGMPTSA itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C crkl_human Crk-like protein CRKL protein protein human Homo sapiens Human MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE DIP protein P46109 original sequence version: 64 DIP protein P46109 original sequence version: 103 DIP protein P46109 original sequence version: 104 DIP protein P46109 original sequence version: 156 294CF1EE2CD44B81 yrcpeptide PSI domain peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YRCTLNSPFFWEDMTHECHA nstpeptide-crkl-1 3 8 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey Region 123-183 Region 123-183 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 235-296 Region 235-296 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction crkl-itgb1-1 1 9 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association yrcpeptide-crkl-1 4 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait in vitro In vitro physical association physical association itgb1-crkl-1 6 9 unspecified role unspecified role bait bait human Homo sapiens Human 10 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association yrcpeptide-crkl-2 2 10 unspecified role unspecified role prey prey Region 123-183 Region 123-183 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 235-296 Region 235-296 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 11 unspecified role unspecified role bait bait in vitro In vitro direct interaction direct interaction crkl-crkl-1 5 10 unspecified role unspecified role prey prey GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 10 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction yrcpeptide-crkl-3 7 10 unspecified role unspecified role prey prey human Homo sapiens Human 11 unspecified role unspecified role bait bait in vitro In vitro physical association physical association