DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132943X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132950X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132941X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132947X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132954X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132945X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132942X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132952X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132946X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132949X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132944X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132953X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132948X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. Nature structural & molecular biology 2009 imex curation Tripathi A., Ren Y., Jeffrey PD., Hughson FM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Tripathi A., Ren Y., Jeffrey PD., Hughson FM. DIP original experiment: DIP-132951X Nature structural & molecular biology 2009 IMEx Structural characterization of Tip20p and Dsl1p, subunits of the Dsl1p vesicle tethering complex. use1_yeast Protein transport protein USE1 USE1 SLT1 Unconventional SNARE in the endoplasmic reticulum protein 1 SNARE-like tail-anchored protein 1 YGL098W protein protein yeast Saccharomyces cerevisiae Baker's yeast MAETSNDPFLSYVLSSKQLTNLNRLRRKAVTKQLGSSDDNKVSEEFLRYQHTYQREAFEYLQTKHDAHKIMESQYEQYQSSSKTRRYSIDLDSVDAVDTESQTEYPNEEFIDRNEDSEAVMELRKRLLGKGQNKGLGYETTKSVDRQIEDQDTLQQDLIQDMSKLVGSLKQGAVAFQSALDEDKQVLGAAEIGIQVASQGLMDVSGKLRKYDKSKLSYLFYITVFIFMILGLVFTFIIIQLFPAL 213ABBF0492E80EF 213ABBF0492E80EF sec39_yeast Protein transport protein SEC39 Dependent on SLY1-20 protein 3 SEC39 DSL3 YLR440C protein protein yeast Saccharomyces cerevisiae Baker's yeast MLEEQLYLLACIFASRADTRNIKKLSTRLGSQSKYLEILCVLWPELDDPKNLLFLRELEEEVQSPEGEETTDEDVIVELLESDSSLIPLIESDTTTRSNRYHELQEFISKKLNNKTLENFEEWLRERILICNEMIPETPLLYSVLWETAKSKVLSTKFIGWVEGVLKPLDHLNKRLHLIFKINEWEKMPDSELFKIIFDGVEDMQGYIGIADVIEDELAPTLSYGKKWETFITEFFNKQQFSLKSDTNYQLFIKLYYSLEKGVKDNSEASRKLQSNVVDILFHNSENLFNLSSLTHKLDELWSILSGFPDEITIEEQKTITALEMKQFMEFFIKCSTKFSFKEIFAITQEEESAQLAHFSSLCHEEFNKANEISSFLQAMYETVLDISKDDKIFTRISMDEKLYSILEILLQMNEFAYIEAIIERFDYSNNTQIYELLVKFFWHFFNNASNGLRKEPEMKKASQTLQIIQKHMSQRAGTNLTKLEVLLEISDKLSHYSINLNKSHNGARDTAFKPSNILEYRDCPLDIISNLLELNPRLYKDLPTTKSLLFGIYDSLSINREGQTGKVEVDLMVLHIDYALVNLDFGTAYELGKQVFEICQEAGQHMMKALGDEHWLTFYQMGKFVDPNWVDNEIPTEIIVLQMSILGRLLEVCPLEEVEIVTSQWSTLELELSARDLVKDKYALDGQNDNKSKVGGIAREIFHNVTNF 8ABD59328A006863 8ABD59328A006863 tip20_yeast Protein transport protein TIP20 YGL145W TIP20 TIP1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNGIDDLLNINDRIKQVQNERNELASKLQNLKQSLASNDTEVALSEVIAQDIIEVGASVEGLEQLRAKYGDLQILNKLEKVAVQQTQMQAGVDKLDSFERQLDELAEQPPDQFTLDDVKALHSKLTSVFATVPQINNIDSQYAAYNKLKSKVTGKYNDVIIQRLATNWSNTFDQKLLEAQWDTQKFASTSVGLVKCLRENSTKLYQLSLLYLPLEEETQNGDSERPLSRSNNNQEPVLWNFKSLANNFNVRFTYHFHATSSSSKIETYFQFLNDYLAENLYKCINIFHDDCNGLTKPVIHEQFINYVLQPIRDKVRSTLFQNDLKTLIVLISQILATDKNLLNSFHYHGLGLVSLISDEVWEKWINYEVEMANRQFINITKNPEDFPKSSQNFVKLINKIYDYLEPFYDLDFDLLVRYKLMTCSLIFMNLTSSYLDYILTVDSLNETRTKEQELYQTMAKLQHVNFVYRKIKSLSSNFIFIQLTDIVNSTESKKYNSLFQNVENDYEKAMSTDMQNSIVHRIQKLLKETLRNYFKISTWSTLEMSVDENIGPSSVPSAELVNSINVLRRLINKLDSMDIPLAISLKVKNELLNVIVNYFTESILKLNKFNQNGLNQFLHDFKSLSSILSLPSHATNYKCMSLHELVKILKLKYDPNNQQFLNPEYIKTGNFTSLKEAYSIKYLKDTKIQDALYRIIYGNIL 3E561DD4C0B9C176 3E561DD4C0B9C176 dsl1_yeast Protein transport protein DSL1 Dependent on SLY1-20 protein 1 YNL258C N0842 DSL1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MESLFPNKGEIIRELLKDPLILKNDSKRSNGSELELDSSDLLQREAILANELNILDNLKTFLNLIKEVKTNLNILELENCYYSLQSLRKKMRNNAAYLKQSFNFQQSISTYVDTLHLELVSTLYKILTNGFWKITENSIQFTPTVEWGKDKVHIEYDTFMDFVAQQYFPKGSLDNQAWFILDMTSADSQEQVRAKLNTIMKEYMNLSRIVSMIKNSIFISGKEISYENEKNILVFSKSSSHGQHCVSTVLTSFEAVCDFMLDGLAFRDRKTLSYELGPLFNTEFTKFVKNNASIILESLDSPLKNLVSVINNKLTRLVAKSEVTNWTHSGKEIQDLLMNKQLYYNLLLDKVLESHISEIRSIFEDPKKSWQNLEVVELTTSNTNTMSEKIGKNDSDVQNEKELHNAVSKDDDWNWEVEDDDADAWGDEIDVNIDDEEEKTNQEKEKEPEEEENAWDEAWAIDENIDDASLENGKEHLKAHDVGSLDKDHIEVTQLPKLFLAISQNFKSSFADSHVDEQYFAYKYNLLQTSYMAMCTANFSHNWCQLYVDMRYLIERDEKLYRIKELTRNLLETKLNMKYRIVCQLIRHQLTEFRENERNPSWDATIEKLLPYILKEIVRPLQKIRGEEGSRYLLSFLNFLYNDCVTKEILKWQIISEVNSENLGELVSLLVNNTDIQLLAKEPSYKKMREKFATMGKFLPLHLKEIMEMFYNGDFYLFATDELIQWIELLFADTPLRRNAIDDIYEIRGTALDD 51EDB17F2C55A917 51EDB17F2C55A917 sec20_yeast Protein transport protein SEC20 SEC20 D9719.4 YDR498C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVVTFLQDLEVLQDALLNNLQKLSAISRRKESGESKHDNKDSFAAIANEHNDEEEEIEFEDLVNIIESKVSDFESVLKCSIVEMTYKYPELKLQWEKSPRYDQCDKLHIVKLDKQMNEDIYAQLVEELDFVLQFVDWFYCYRLKVKEILRQHHKRDLAWNDEKRDRAIKFHAVDYDKLHQGTSSSSSLTSTSMEKASTREKLLSKTKQLTNNLVRGNQILQSGILQSDLNLDELRAQTNSLTQIDDKYTQFETVFKKTADLVKVLENASHQEKRDVYLSLGFLLCCVSWVLWRRIFKLPVKLGLWLLFKFFKGILVTLGLVKSYAGSSSSLQAPSLVLNAPILATTTTSSATSVEPFASVSAVSSIQRAVDEAVDRIVSHDEL 9BF8C748B8A8F589 9BF8C748B8A8F589 dsl1p-tip20p-1 3 18 unspecified role unspecified role unspecified role unspecified role Region 1-361 Region 1-361 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction dsl1p-sec20p-1 10 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 19 unspecified role unspecified role unspecified role unspecified role Region 1-275 Region 1-275 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association dsl1p-sec20p-2 8 19 unspecified role unspecified role unspecified role unspecified role Region 1-275 Region 1-275 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 1-167 Region 1-167 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association dsl1p-sec39p-1 6 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role Region 1-81 Region 1-81 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli physical association physical association dsl1p-tip20p-2 7 18 unspecified role unspecified role unspecified role unspecified role Region 1-56 Region 1-56 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P53847:p.Leu48Glu L48E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L48E'. Wed May 02 19:16:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu48glu' Tue Jun 30 17:31:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu48Glu' P53847:p.Leu55Glu L55E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L E This feature has been corrected as a result of our quality control procedures. The original label was 'L55E'. Wed May 02 14:53:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu55glu' Tue Jun 30 17:04:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu55Glu' P53847:p.Leu58Asp L58D mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L D This feature has been corrected as a result of our quality control procedures. The original label was 'L58D'. Wed May 02 18:44:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu58asp' Tue Jun 30 18:46:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu58Asp' ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role Region 1-81 Region 1-81 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain P33891:p.[Ile10Asp;Leu28Glu] I10D/L28E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L E certain certain sequence position certain certain certain sequence position certain I D This feature has been corrected as a result of our quality control procedures. The original label was 'I10D/L28E'. Wed May 02 18:39:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile10asp,leu28glu' Tue Jun 30 18:53:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ile10Asp;Leu28Glu]' P33891:p.Val17Glu V17E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V E This feature has been corrected as a result of our quality control procedures. The original label was 'V17E'. Wed May 02 17:22:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val17glu' Tue Jun 30 16:07:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val17Glu' ecolx Escherichia coli direct interaction direct interaction dsl1p-sec39p-2 9 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role Region 340-754 Region 340-754 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dsl1p-sec39p-3 14 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 1-167 Region 1-167 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association tip20p-dsl1p-1 1 18 unspecified role unspecified role prey prey Region 1-361 Region 1-361 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role bait bait Region 1-43 Region 1-43 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction sec39p-use1p-1 2 15 unspecified role unspecified role unspecified role unspecified role Region 1-167 Region 1-167 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction dsl1p-sec39p-4 4 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 18 unspecified role unspecified role unspecified role unspecified role Region 362-754 Region 362-754 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association dsl1p-sec39p-5 12 18 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 1-167 Region 1-167 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association sec20p-tip20p-1 13 19 unspecified role unspecified role unspecified role unspecified role Region 1-275 Region 1-275 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction sec20p-tip20p-2 5 19 unspecified role unspecified role unspecified role unspecified role Region 1-275 Region 1-275 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role Region 82-701 Region 82-701 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction dsl1p-tip20p-3 11 18 unspecified role unspecified role unspecified role unspecified role Region 1-361 Region 1-361 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 17 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction