DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The influence of catalysis on mad2 activation dynamics. The influence of catalysis on mad2 activation dynamics. PLoS biology 2009 imex curation Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. Only protein-protein interactions in vitro In vitro pull down pull down weight by staining molecular weight estimation by staining weight by staining Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. DIP original experiment: DIP-132547X PLoS biology 2009 IMEx The influence of catalysis on mad2 activation dynamics. The influence of catalysis on mad2 activation dynamics. The influence of catalysis on mad2 activation dynamics. PLoS biology 2009 imex curation Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. DIP original experiment: DIP-132549X PLoS biology 2009 IMEx The influence of catalysis on mad2 activation dynamics. The influence of catalysis on mad2 activation dynamics. The influence of catalysis on mad2 activation dynamics. PLoS biology 2009 imex curation Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Simonetta M., Manzoni R., Mosca R., Mapelli M., Massimiliano L., Vink M., Novak B., Musacchio A., Ciliberto A. DIP original experiment: DIP-132548X PLoS biology 2009 IMEx The influence of catalysis on mad2 activation dynamics. cdc20_human Cell division cycle protein 20 homolog CDC20 p55CDC protein protein human Homo sapiens Human MAQFAFESDLHSLLQLDAPIPNAPPARWQRKAKEAAGPAPSPMRAANRSHSAGRTPGRTPGKSSSKVQTTPSKPGGDRYIPHRSAAQMEVASFLLSKENQPENSQTPTKKEHQKAWALNLNGFDVEEAKILRLSGKPQNAPEGYQNRLKVLYSQKATPGSSRKTCRYIPSLPDRILDAPEIRNDYYLNLVDWSSGNVLAVALDNSVYLWSASSGDILQLLQMEQPGEYISSVAWIKEGNYLAVGTSSAEVQLWDVQQQKRLRNMTSHSARVGSLSWNSYILSSGSRSGHIHHHDVRVAEHHVATLSGHSQEVCGLRWAPDGRHLASGGNDNLVNVWPSAPGEGGWVPLQTFTQHQGAVKAVAWCPWQSNVLATGGGTSDRHIRIWNVCSGACLSAVDAHSQVCSILWSPHYKELISGHGFAQNQLVIWKYPTMAKVAELKGHTSRVLSLTMSPDGATVASAAADETLRLWRCFELDPARRREREKASAAKSSLIHQGIR DIP protein Q12834 original sequence version: 132 DIP protein Q12834 original sequence version: 74 DIP protein Q12834 original sequence version: 100 DIP protein Q12834 original sequence version: 118 DIP protein Q12834 original sequence version: 125 DIP protein Q12834 original sequence version: 114 DIP protein Q12834 original sequence version: 147 DIP protein Q12834 original sequence version: 162 FD5C967AF84089E8 md2l1_human Mitotic spindle assembly checkpoint protein MAD2A MAD2L1 MAD2 Mitotic arrest deficient 2-like protein 1 protein protein human Homo sapiens Human MALQLSREQGITLRGSAEIVAEFFSFGINSILYQRGIYPSETFTRVQKYGLTLLVTTDLELIKYLNNVVEQLKDWLYKCSVQKLVVVISNIESGEVLERWQFDIECDKTAKDDSAPREKSQKAIQDEIRSVIRQITATVTFLPLLEVSCSFDLLIYTDKDLVVPEKWEESGPQFITNSEEVRLRSFTTTIHKVNSMVAYKIPVND DIP protein Q13257 original sequence version: 133 DIP protein Q13257 original sequence version: 78 DIP protein Q13257 original sequence version: 105 DIP protein Q13257 original sequence version: 120 DIP protein Q13257 original sequence version: 127 DIP protein Q13257 original sequence version: 116 DIP protein Q13257 original sequence version: 150 DIP protein Q13257 original sequence version: 156 DIP protein Q13257 original sequence version: 134 B8DCBF0043836764 cdc20-mad2l1-10 1 4 unspecified role unspecified role bait bait Region 111-138 Region 111-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction cdc20-mad2l1-11 2 4 unspecified role unspecified role bait bait Region 111-138 Region 111-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 5 unspecified role unspecified role prey prey Alexa488-tag Alexa488-tag alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli physical association physical association cdc20-mad2l1-12 3 4 unspecified role unspecified role bait bait Region 111-138 Region 111-138 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 5 unspecified role unspecified role prey prey Alexa488-tag Alexa488-tag alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction