DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133607X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133610X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133608X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. Proceedings of the National Academy of Sciences of the United States of America 2009 imex curation Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Suzuki T., Elias BC., Seth A., Shen L., Turner JR., Giorgianni F., Desiderio D., Guntaka R., Rao R. DIP original experiment: DIP-133609X Proceedings of the National Academy of Sciences of the United States of America 2009 IMEx PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. ocln_canlf Occludin OCLN protein protein canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris MSSRPFESPPPYRPDEFKPNHYAPSNDVYGGDMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLVIVMCIAIFGCVASTLAWDRGYGTGLMGGSIGYPYGSGFGSYGTGYGYGFGYGYGYGGYTDPRAAKGFLLAMVAFCFIAALVIFVTSVIRSDISRTRRYYLTVIILSAFLGVMMFIATIVYIMGVNPTAQASGSLYSSQIYAMCNQFYASTATGLYMDQYLYHYCVVDPQEAIAIVLGFMVIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDMPPPPSDYVERVDSPMAYSSNGKVNDKRLYPESSYKSTPVPEVVQELPATSPADDFRQPRYSSSGHLEPPSKRAPSKGRTGRPKRLEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQAELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSPDYKNKRNYCKQLKSKLSHIKKMVGDYDRQKT DIP protein Q28269 original sequence version: 62 kpcl_human Protein kinase C eta type PRKCH PKCL PRKCL PKC-L nPKC-eta protein protein human Homo sapiens Human MSSGTMKFNGYLRVRIGEAVGLQPTRWSLRHSLFKKGHQLLDPYLTVSVDQVRVGQTSTKQKTNKPTYNEEFCANVTDGGHLELAVFHETPLGYDHFVANCTLQFQELLRTTGASDTFEGWVDLEPEGKVFVVITLTGSFTEATLQRDRIFKHFTRKRQRAMRRRVHQINGHKFMATYLRQPTYCSHCREFIWGVFGKQGYQCQVCTCVVHKRCHHLIVTACTCQNNINKVDSKIAEQRFGINIPHKFSIHNYKVPTFCDHCGSLLWGIMRQGLQCKICKMNVHIRCQANVAPNCGVNAVELAKTLAGMGLQPGNISPTSKLVSRSTLRRQGKESSKEGNGIGVNSSNRLGIDNFEFIRVLGKGSFGKVMLARVKETGDLYAVKVLKKDVILQDDDVECTMTEKRILSLARNHPFLTQLFCCFQTPDRLFFVMEFVNGGDLMFHIQKSRRFDEARARFYAAEIISALMFLHDKGIIYRDLKLDNVLLDHEGHCKLADFGMCKEGICNGVTTATFCGTPDYIAPEILQEMLYGPAVDWWAMGVLLYEMLCGHAPFEAENEDDLFEAILNDEVVYPTWLHEDATGILKSFMTKNPTMRLGSLTQGGEHAILRHPFFKEIDWAQLNHRQIEPPFRPRIKSREDVSNFDPDFIKEEPVLTPIDEGHLPMINQDEFRNFSYVSPELQP This sequence has been withdrawn from Uniprot. The sequence has been withdrawn from uniprot. 0A29806CE31912F2 q16625-1 Occludin OCLN WT-OCLN TM4(+) protein protein human Homo sapiens Human MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT DIP protein Q16625-1 original sequence version: 135 DIP protein Q16625 original sequence version: 92 pkceta-ocln-1 1 5 unspecified role unspecified role prey prey canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris 6 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris physical association physical association ocln-pkceta-1 2 6 enzyme enzyme unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 378-522 Region 378-522 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q16625-1:p.Thr403Ala T403A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T403A'. Wed May 02 16:52:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr403ala' Tue Jun 30 15:56:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr403Ala' Q16625-1:p.Thr404Ala T404A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T404A'. Wed May 02 18:48:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr404ala' Tue Jun 30 18:34:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr404Ala' Q16625-1:p.Thr438Ala T438A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A This feature has been corrected as a result of our quality control procedures. The original label was 'T438A'. Wed May 02 17:37:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr438ala' Tue Jun 30 20:08:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr438Ala' pT403 pT403 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain pT404 pT404 opthr O-phospho-L-threonine (2S,3R)-2-amino-3-(phosphonooxy)butanoic acid 2-amino-3-hydroxybutanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)butanoic acid MOD_RES Phosphothreonine O-phospho-L-threonine O-phosphorylated L-threonine O3-phosphothreonine threonine phosphate ester OPThr Phospho Phospho Threonyl Phosphorylation phosphorylationt modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain pS408 pS408 opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations modified residue ms mass detection of residue modification modified residue ms certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli phosphorylation phosphorylation reaction phosphorylation ocln-pkceta-2 3 5 unspecified role unspecified role bait bait canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris 6 unspecified role unspecified role prey prey GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal canlf Canis familiaris (dog) Canis lupus familiaris Dog Canis familiaris physical association physical association ocln-pkceta-3 4 6 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm 7 unspecified role unspecified role bait bait Region 378-522 Region 378-522 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction