DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249962X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249960X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249958X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249957X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249955X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions ecolx Escherichia coli comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249954X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting tag fluorescence tag visualisation by fluorescence tag fluorescence Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249959X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249961X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249953X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. Nature 2009 imex curation Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel predetermined predetermined participant predetermined Jovanovic-Talisman T., Tetenbaum-Novatt J., McKenney AS., Zilman A., Peters R., Rout MP., Chait BT. DIP original experiment: DIP-249956X Nature 2009 IMEx Artificial nanopores that mimic the transport selectivity of the nuclear pore complex. imb1_yeast Importin subunit beta-1 KAP95 YLR347C L8300.15 Karyopherin subunit beta-1 Karyopherin-95 Importin-95 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSTAEFAQLLENSILSPDQNIRLTSETQLKKLSNDNFLQFAGLSSQVLIDENTKLEGRILAALTLKNELVSKDSVKTQQFAQRWITQVSPEAKNQIKTNALTALVSIEPRIANAAAQLIAAIADIELPHGAWPELMKIMVDNTGAEQPENVKRASLLALGYMCESADPQSQALVSSSNNILIAIVQGAQSTETSKAVRLAALNALADSLIFIKNNMEREGERNYLMQVVCEATQAEDIEVQAAAFGCLCKIMSLYYTFMKPYMEQALYALTIATMKSPNDKVASMTVEFWSTICEEEIDIAYELAQFPQSPLQSYNFALSSIKDVVPNLLNLLTRQNEDPEDDDWNVSMSAGACLQLFAQNCGNHILEPVLEFVEQNITADNWRNREAAVMAFGSIMDGPDKVQRTYYVHQALPSILNLMNDQSLQVKETTAWCIGRIADSVAESIDPQQHLPGVVQACLIGLQDHPKVATNCSWTIINLVEQLAEATPSPIYNFYPALVDGLIGAANRIDNEFNARASAFSALTTMVEYATDTVAETSASISTFVMDKLGQTMSVDENQLTLEDAQSLQELQSNILTVLAAVIRKSPSSVEPVADMLMGLFFRLLEKKDSAFIEDDVFYAISALAASLGKGFEKYLETFSPYLLKALNQVDSPVSITAVGFIADISNSLEEDFRRYSDAMMNVLAQMISNPNARRELKPAVLSVFGDIASNIGADFIPYLNDIMALCVAAQNTKPENGTLEALDYQIKVLEAVLDAYVGIVAGLHDKPEALFPYVGTIFQFIAQVAEDPQLYSEDATSRAAVGLIGDIAAMFPDGSIKQFYGQDWVIDYIKRTRSGQLFSQATKDTARWAREQQKRQLSL ED530F5313E75B06 ED530F5313E75B06 ntf2_human Nuclear transport factor 2 Placental protein 15 NUTF2 NTF2 protein protein human Homo sapiens Human MGDKPIWEQIGSSFIQHYYQLFDNDRTQLGAIYIDASCLTWEGQQFQGKAAIVEKLSSLPFQKIQHSITAQDHQPTPDSCIISMVVGQLKADEDPIMGFHQMFLLKNINDAWVCTNDMFRLALHNFG DIP protein P61970 original sequence version: 86 817752F20E262FD3 ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL DIP protein P62826 original sequence version: 138 DIP protein P62826 original sequence version: 67 DIP protein P62826 original sequence version: 93 DIP protein P62826 original sequence version: 125 DIP protein P62826 original sequence version: 96 D5C9B7275C34BCE0 ima1_yeast Importin subunit alpha SRP1 KAP60 YNL189W N1606 Karyopherin subunit alpha Karyopherin-60 Serine-rich RNA polymerase I suppressor protein protein protein yeast Saccharomyces cerevisiae Baker's yeast MDNGTDSSTSKFVPEYRRTNFKNKGRFSADELRRRRDTQQVELRKAKRDEALAKRRNFIPPTDGADSDEEDESSVSADQQFYSQLQQELPQMTQQLNSDDMQEQLSATVKFRQILSREHRPPIDVVIQAGVVPRLVEFMRENQPEMLQLEAAWALTNIASGTSAQTKVVVDADAVPLFIQLLYTGSVEVKEQAIWALGNVAGDSTDYRDYVLQCNAMEPILGLFNSNKPSLIRTATWTLSNLCRGKKPQPDWSVVSQALPTLAKLIYSMDTETLVDACWAISYLSDGPQEAIQAVIDVRIPKRLVELLSHESTLVQTPALRAVGNIVTGNDLQTQVVINAGVLPALRLLLSSPKENIKKEACWTISNITAGNTEQIQAVIDANLIPPLVKLLEVAEYKTKKEACWAISNASSGGLQRPDIIRYLVSQGCIKPLCDLLEIADNRIIEVTLDALENILKMGEADKEARGLNINENADFIEKAGGMEKIFNCQQNENDKIYEKAYKIIETYFGEEEDAVDETMAPQNAGNTFGFGSNVNQQFNFN 8D3A0CB76F2E7C00 8D3A0CB76F2E7C00 nsp1_yeast Nucleoporin NSP1 NSP1 YJL041W J1207 Nuclear pore protein NSP1 Nucleoskeletal-like protein p110 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNFNTPQQNKTPFSFGTANNNSNTTNQNSSTGAGAFGTGQSTFGFNNSAPNNTNNANSSITPAFGSNNTGNTAFGNSNPTSNVFGSNNSTTNTFGSNSAGTSLFGSSSAQQTKSNGTAGGNTFGSSSLFNNSTNSNTTKPAFGGLNFGGGNNTTPSSTGNANTSNNLFGATANANKPAFSFGATTNDDKKTEPDKPAFSFNSSVGNKTDAQAPTTGFSFGSQLGGNKTVNEAAKPSLSFGSGSAGANPAGASQPEPTTNEPAKPALSFGTATSDNKTTNTTPSFSFGAKSDENKAGATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKPDENKASATSKPAFSFGAKPEEKKDDNSSKPAFSFGAKSNEDKQDGTAKPAFSFGAKPAEKNNNETSKPAFSFGAKSDEKKDGDASKPAFSFGAKSDEKKDSDSSKPAFSFGTKSNEKKDSGSSKPAFSFGAKPDEKKNDEVSKPAFSFGAKANEKKESDESKSAFSFGSKPTGKEEGDGAKAAISFGAKPEEQKSSDTSKPAFTFGAQKDNEKKTEESSTGKSTADVKSSDSLKLNSKPVELKPVSLDNKTLDDLVTKWTNQLTESASHFEQYTKKINSWDQVLVKGGEQISQLYSDAVMAEHSQNKIDQSLQYIERQQDELENFLDNFETKTEALLSDVVSTSSGAAANNNDQKRQQAYKTAQTLDENLNSLSSNLSSLIVEINNVSNTFNKTTNIDINNEDENIQLIKILNSHFDALRSLDDNSTSLEKQINSIKK A258003CB1917810 A258003CB1917810 nu100_yeast Nucleoporin NUP100/NSP100 NUP100 NSP100 YKL068W YKL336 Nuclear pore protein NUP100/NSP100 protein protein yeast Saccharomyces cerevisiae Baker's yeast MFGNNRPMFGGSNLSFGSNTSSFGGQQSQQPNSLFGNSNNNNNSTSNNAQSGFGGFTSAAGSNSNSLFGNNNTQNNGAFGQSMGATQNSPFGSLNSSNASNGNTFGGSSSMGSFGGNTNNAFNNNSNSTNSPFGFNKPNTGGTLFGSQNNNSAGTSSLFGGQSTSTTGTFGNTGSSFGTGLNGNGSNIFGAGNNSQSNTTGSLFGNQQSSAFGTNNQQGSLFGQQSQNTNNAFGNQNQLGGSSFGSKPVGSGSLFGQSNNTLGNTTNNRNGLFGQMNSSNQGSSNSGLFGQNSMNSSTQGVFGQNNNQMQINGNNNNSLFGKANTFSNSASGGLFGQNNQQQGSGLFGQNSQTSGSSGLFGQNNQKQPNTFTQSNTGIGLFGQNNNQQQQSTGLFGAKPAGTTGSLFGGNSSTQPNSLFGTTNVPTSNTQSQQGNSLFGATKLTNMPFGGNPTANQSGSGNSLFGTKPASTTGSLFGNNTASTTVPSTNGLFGNNANNSTSTTNTGLFGAKPDSQSKPALGGGLFGNSNSNSSTIGQNKPVFGGTTQNTGLFGATGTNSSAVGSTGKLFGQNNNTLNVGTQNVPPVNNTTQNALLGTTAVPSLQQAPVTNEQLFSKISIPNSITNPVKATTSKVNADMKRNSSLTSAYRLAPKPLFAPSSNGDAKFQKWGKTLERSDRGSSTSNSITDPESSYLNSNDLLFDPDRRYLKHLVIKNNKNLNVINHNDDEASKVKLVTFTTESASKDDQASSSIAASKLTEKAHSPQTDLKDDHDESTPDPQSKSPNGSTSIPMIENEKISSKVPGLLSNDVTFFKNNYYISPSIETLGNKSLIELRKINNLVIGHRNYGKVEFLEPVDLLNTPLDTLCGDLVTFGPKSCSIYENCSIKPEKGEGINVRCRVTLYSCFPIDKETRKPIKNITHPLLKRSIAKLKENPVYKFESYDPVTGTYSYTIDHPVLT D3985F9901BBAA51 D3985F9901BBAA51 nutf2-1 9 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nutf2-rangdp-1 10 13 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nsp1fg-kap95-1 7 11 unspecified role unspecified role prey prey Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 30-591 Region 30-591 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nutf2-2 6 12 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nup100fg-kap95-1 8 16 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role prey prey Alexa488-label Alexa488-label alexa 488 label alexa 488 label undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction ibb-kap95-1 4 14 unspecified role unspecified role unspecified role unspecified role Region 1-65 Region 1-65 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain eGFP-tag eGFP-tag egfp tag enhanced green fluorescent protein tag enhanced green fluorescent protein EGFP enhanced green fluorescent protein EGFP egfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction ibb-kap95-2 5 14 unspecified role unspecified role unspecified role unspecified role Region 1-65 Region 1-65 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction nup100fg-nutf2-1 2 16 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 12 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction nup100fg-kap95-2 1 16 unspecified role unspecified role bait bait Region 1-570 Region 1-570 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 11 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction nsp1fg-nutf2-1 3 12 unspecified role unspecified role prey prey YFP-tag YFP-tag yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 15 unspecified role unspecified role bait bait Region 30-591 Region 30-591 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction