DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Nature 2009 imex curation Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. DIP original experiment: DIP-249769X Nature 2009 IMEx Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Nature 2009 imex curation Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. DIP original experiment: DIP-249770X Nature 2009 IMEx Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Nature 2009 imex curation Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. Only protein-protein interactions ecolx Escherichia coli anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. DIP original experiment: DIP-249767X Nature 2009 IMEx Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Nature 2009 imex curation Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. Only protein-protein interactions in vitro In vitro solid phase assay solid phase assay predetermined predetermined participant predetermined Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. DIP original experiment: DIP-249768X Nature 2009 IMEx Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. Nature 2009 imex curation Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column ms participant Identification by mass spectrometry ms participant Roy K., Hilliard GM., Hamilton DJ., Luo J., Ostmann MM., Fleckenstein JM. DIP original experiment: DIP-249766X Nature 2009 IMEx Enterotoxigenic Escherichia coli EtpA mediates adhesion between flagella and host cells. q29xt7_ecolx etpA protein protein ecolx Escherichia coli MNRIYKLKFDKRRNELVVVSEITTGVGNAKATGSVEGEKSPRRGVRAMALSLLSGMMIMAHPAMSANLPTGGQIVAGSGSIQTPSGNQMNIHQNSQNMVANWNSFDIGKGNTVQFDQPSSSAVALNRVVGGGESQIMGNLKANGQVFLVNPNGVLFGEGASVSTSGFVASTRDIKNDDFMNRRYTFSGGQKAGAAIVNQGELTTNAGGYIVLAADRVSNSGTIRTPGGKTVLAASERITLQLDNGGLMSVQVTGDVVNALVENRGLVSARDGQVYLTALGRGMLMNTVLNVSGVVEASGMHRQDGNIVLDGGDSGVVHLSGTLQADNASGQGGKVVVQGKNILLDKGSNITATGGQGGGEVYVGGGWQGKDSNIRNADKVVMQGGARIDVSATQQGNGGTAVLWSDSYTNFHGQISAKGGETGGNGGRVETSSHGNLQAFGTVSASAKKGKAGNWLLDSADITIVNGSNVSKTETTQSPPHTQFAPTAAGSAVSNTSINNRLNNGTSVTILTHRTRTGTAQGGNITVNAAINKSNGSDVNLTLQAGGNITVNNSITSTEGKLNVNLSGARTSNGSITISNNANITTNGGDITVGTTNTSNRVNISINNTTLNASNGNIQLTGTGTDSGILFAGNNRLTASNIALTGNSTSGNAINLTGTATLNATNNITLTGSSTSGNAINLKGNNTLTASNITLTGESTSGNAINLTDTTGTTTLNATNNITMQGTRVQIKHSNITAGNFALNATVAGSEISNTTLTATNNINLAAKTNSASSGVYLKDARITSTNGSITANGTATANGKATHLDGNVTLNASNGRIKLTGNGHGSASGILFAGNNRLTASNIALTGNSTSGNAINLTGTATLNATNDITLTGSSTSGNAINLTGTATLNATNNITLTGSSTSGNAINLKGNNTLTASNITLTGESTSGNAINLTDTTGTTTLNATNNITMQGTRVQIKHSNITAGNFALNATVAGSEISNTTLTATNNINLAAKTNSASSGVYLKDARITSTNGSITANGTATANGKATHLDGNVTLNASNGRIKLTGNGHGSASGILFAGNNRLTASNIALTGNSTSGNAINLTGTATLNATNDITLTGSSTSGNAINLTGTATLNATNNITLTGSSTSGNAINLKGNNTLTASNITLTGESTSGNAINLTDTTGTTTLNATNNITMQGTRVQIKHSNITAGNFALNATVAGSEISNTTLTATNNINLAAKTNSASSGVYLKDARITSTNGSITANGTATANGKATHLDGNVTLNASNGRIKLTGNGHGSASGILFAGNNRLTASNIALTGNSTSGNAINLTGTATLNATNDITLTGSSTSGNAINLTGTATLNATNNITLTGSSTSGNAINLKGNNTLTASNITLTGESTSGNAINLTDTTGTTTLNATNNITMQGTRVQIKHSNITAGNFALNATVAGSEISNTTLTATNNINLAAKTNSASSGVYLKDARITSTNGSITTNGTATANGKATHLDGNVTLNASNGRIKLTGNGHGSASGILFAGNNRLTASNIALTGNSTSGNAINLTGTATLNATNDITLTGSSTSGNAINLTGTATLNATNNITLTGSSTSGNAINLKGNNTLTASNITLTGESTSGNAINLTDTTGTTTLNATNNITMQGTRVQIKHSNITAGNFALNATVAGSEISNTTLTATNNINLAAKTNSASSGVYLKDARITSTNGSITANGTAPANDNATYLDGNVTLNASNGSIKLTGNGNGSTSGILFAGNNTLTASNITLTGNSEVYWQ DIP protein Q29XT7 original sequence version: 23 flic_ecoli Flagellin fliC flaF hag b1923 JW1908 protein protein ecoli Escherichia coli (strain K12) MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDDAAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRVRELTVQATTGTNSESDLSSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKNGSMKIQVGANDNQTITIDLKQIDAKTLGLDGFSVKNNDTVTTSAPVTAFGATTTNNIKLTGITLSTEAATDTGGTNPASIEGVYTDNGNDYYAKITGGDNDGKYYAVTVANDGTVTMATGATANATVTDANTTKATTITSGGTPVQIDNTAGSATANLGAVSLVKLQDSKGNDTDTYALKDTNGNLYAADVNETTGAVSVKTITYTDSSGAASSPTAVKLGGDDGKTEVVDIDGKTYDSADLNGGNLQTGLTAGGEALTAVANGKTTDPLKALDDAIASVDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG 14A0ED3E68E326A1 14A0ED3E68E326A1 etpa-flic-1 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction flic-etpa-1 1 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Region 1-173 Region 1-173 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction etpa-flic-2 3 7 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role bait bait ecolx Escherichia coli physical association physical association flic-etpa-2 4 7 unspecified role unspecified role bait bait ecolx Escherichia coli 6 unspecified role unspecified role prey prey Biotin-tag Biotin-tag biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction flic-etpa-3 2 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 6 unspecified role unspecified role prey prey Region 1-560 Region 1-560 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction