DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Nature 2008 imex curation Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions in vitro In vitro emsa electrophoretic mobility shift assay Gel retardation assay band shift band shift Gel retardation assay emsa predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249984X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Nature 2008 imex curation Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249983X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. Nature 2008 imex curation Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Chandra V., Huang P., Hamuro Y., Raghuram S., Wang Y., Burris TP., Rastinejad F. DIP original experiment: DIP-249982X Nature 2008 IMEx Structure of the intact PPAR-gamma-RXR- nuclear receptor complex on DNA. rxra_human Retinoic acid receptor RXR-alpha RXRA NR2B1 Retinoid X receptor alpha Nuclear receptor subfamily 2 group B member 1 protein protein human Homo sapiens Human MDTKHFLPLDFSTQVNSSLTSPTGRGSMAAPSLHPSLGPGIGSPGQLHSPISTLSSPINGMGPPFSVISSPMGPHSMSVPTTPTLGFSTGSPQLSSPMNPVSSSEDIKPPLGLNGVLKVPAHPSGNMASFTKHICAICGDRSSGKHYGVYSCEGCKGFFKRTVRKDLTYTCRDNKDCLIDKRQRNRCQYCRYQKCLAMGMKREAVQEERQRGKDRNENEVESTSSANEDMPVERILEAELAVEPKTETYVEANMGLNPSSPNDPVTNICQAADKQLFTLVEWAKRIPHFSELPLDDQVILLRAGWNELLIASFSHRSIAVKDGILLATGLHVHRNSAHSAGVGAIFDRVLTELVSKMRDMQMDKTELGCLRAIVLFNPDSKGLSNPAEVEALREKVYASLEAYCKHKYPEQPGRFAKLLLRLPALRSIGLKCLEHLFFFKLIGDTPIDTFLMEMLEAPHQMT DIP protein P19793 original sequence version: 177 DIP protein P19793 original sequence version: 107 DIP protein P19793 original sequence version: 153 DIP protein P19793 original sequence version: 131 DIP protein P19793 original sequence version: 136 DIP protein P19793 original sequence version: 169 DIP protein P19793 original sequence version: 161 DIP protein P19793 original sequence version: 139 7F952B580AD84C42 p37231-2 Peroxisome proliferator-activated receptor gamma PPARG NR1C3 Nuclear receptor subfamily 1 group C member 3 PPARgamma1(wt) protein protein human Homo sapiens Human MTMVDTEMPFWPTNFGISSVDLSVMEDHSHSFDIKPFTTVDFSSISTPHYEDIPFTRTDPVVADYKYDLKLQEYQSAIKVEPASPPYYSEKTQLYNKPHEEPSNSLMAIECRVCGDKASGFHYGVHACEGCKGFFRRTIRLKLIYDRCDLNCRIHKKSRNKCQYCRFQKCLAVGMSHNAIRFGRMPQAEKEKLLAEISSDIDQLNPESADLRALAKHLYDSYIKSFPLTKAKARAILTGKTTDKSPFVIYDMNSLMMGEDKIKFKHITPLQEQSKEVAIRIFQGCQFRSVEAVQEITEYAKSIPGFVNLDLNDQVTLLKYGVHEIIYTMLASLMNKDGVLISEGQGFMTREFLKSLRKPFGDFMEPKFEFAVKFNALELDDSDLAIFIAVIILSGDRPGLLNVKPIEDIQDNLLQALELQLKLNHPESSQLFAKLLQKMTDLRQIVTEHVQLLQVIKKTETDMSLHPLLQEIYKDLY DIP protein P37231-2 original sequence version: 128 1061C2074B739E0A coactivator peptide Coactivator peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EKHKILHRLLQDSY pparg-rxra-1 1 5 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 4 unspecified role unspecified role unspecified role unspecified role in vitro In vitro physical association physical association coactivator peptide-pparg-1 3 6 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli physical association physical association coactivator peptide-pparg-2 2 6 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction