DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. DIP original experiment: DIP-251164X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. DIP original experiment: DIP-113749X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. DIP original experiment: DIP-251163X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Structural basis of sequence-specific collagen recognition by SPARC. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hohenester E., Sasaki T., Giudici C., Farndale RW., Bächinger HP. DIP original experiment: DIP-113750X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis of sequence-specific collagen recognition by SPARC. p02461-1 Collagen alpha-1(III) chain COL3A1 protein protein human Homo sapiens Human MMSFVQKGSWLLLALLHPTIILAQQEAVEGGCSHLGQSYADRDVWKPEPCQICVCDSGSVLCDDIICDDQELDCPNPEIPFGECCAVCPQPPTAPTRPPNGQGPQGPKGDPGPPGIPGRNGDPGIPGQPGSPGSPGPPGICESCPTGPQNYSPQYDSYDVKSGVAVGGLAGYPGPAGPPGPPGPPGTSGHPGSPGSPGYQGPPGEPGQAGPSGPPGPPGAIGPSGPAGKDGESGRPGRPGERGLPGPPGIKGPAGIPGFPGMKGHRGFDGRNGEKGETGAPGLKGENGLPGENGAPGPMGPRGAPGERGRPGLPGAAGARGNDGARGSDGQPGPPGPPGTAGFPGSPGAKGEVGPAGSPGSNGAPGQRGEPGPQGHAGAQGPPGPPGINGSPGGKGEMGPAGIPGAPGLMGARGPPGPAGANGAPGLRGGAGEPGKNGAKGEPGPRGERGEAGIPGVPGAKGEDGKDGSPGEPGANGLPGAAGERGAPGFRGPAGPNGIPGEKGPAGERGAPGPAGPRGAAGEPGRDGVPGGPGMRGMPGSPGGPGSDGKPGPPGSQGESGRPGPPGPSGPRGQPGVMGFPGPKGNDGAPGKNGERGGPGGPGPQGPPGKNGETGPQGPPGPTGPGGDKGDTGPPGPQGLQGLPGTGGPPGENGKPGEPGPKGDAGAPGAPGGKGDAGAPGERGPPGLAGAPGLRGGAGPPGPEGGKGAAGPPGPPGAAGTPGLQGMPGERGGLGSPGPKGDKGEPGGPGADGVPGKDGPRGPTGPIGPPGPAGQPGDKGEGGAPGLPGIAGPRGSPGERGETGPPGPAGFPGAPGQNGEPGGKGERGAPGEKGEGGPPGVAGPPGGSGPAGPPGPQGVKGERGSPGGPGAAGFPGARGLPGPPGSNGNPGPPGPSGSPGKDGPPGPAGNTGAPGSPGVSGPKGDAGQPGEKGSPGAQGPPGAPGPLGIAGITGARGLAGPPGMPGPRGSPGPQGVKGESGKPGANGLSGERGPPGPQGLPGLAGTAGEPGRDGNPGSDGLPGRDGSPGGKGDRGENGSPGAPGAPGHPGPPGPVGPAGKSGDRGESGPAGPAGAPGPAGSRGAPGPQGPRGDKGETGERGAAGIKGHRGFPGNPGAPGSPGPAGQQGAIGSPGPAGPRGPVGPSGPPGKDGTSGHPGPIGPPGPRGNRGERGSEGSPGHPGQPGPPGPPGAPGPCCGGVGAAAIAGIGGEKAGGFAPYYGDEPMDFKINTDEIMTSLKSVNGQIESLISPDGSRKNPARNCRDLKFCHPELKSGEYWVDPNQGCKLDAIKVFCNMETGETCISANPLNVPRKHWWTDSSAEKKHVWFGESMDGGFQFSYGNPELPEDVLDVHLAFLRLLSSRASQNITYHCKNSIAYMDQASGNVKKALKLMGSNEGEFKAEGNSKFTYTVLEDGCTKHTGEWSKTVFEYRTRKAVRLPIVDIAPYDIGGPDQEFGVDVGPVCFL DIP protein P02461-1 original sequence version: 111 sprc_human SPARC ON Basement-membrane protein 40 Osteonectin Secreted protein acidic and rich in cysteine SPARC protein protein human Homo sapiens Human MRAWIFFLLCLAGRALAAPQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKVCELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALDEWAGCFGIKQKDIDKDLVI DIP protein P09486 original sequence version: 106 B914599F79705945 col3a1-sparc-1 2 5 unspecified role unspecified role unspecified role unspecified role Region 564-590 Region 564-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 53-195/204-286 Region 53-195/204-286 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction col3a1-sparc-2 4 5 unspecified role unspecified role unspecified role unspecified role Region 564-590 Region 564-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 53-195/204-286 Region 53-195/204-286 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction col3a1-sparc-3 3 5 unspecified role unspecified role unspecified role unspecified role Region 564-590 Region 564-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 53-195/204-286 Region 53-195/204-286 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction 1 nodeMerge04 2012-12-10 col3a1-sparc-4 1 5 unspecified role unspecified role unspecified role unspecified role Region 564-590 Region 564-590 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro 6 unspecified role unspecified role unspecified role unspecified role Region 53-195/204-286 Region 53-195/204-286 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain human Homo sapiens Human direct interaction direct interaction 1 nodeMerge04 2012-12-10