DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions bovin Bos taurus (Bovine) Bovine anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260485X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260486X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260487X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting predetermined predetermined participant predetermined Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260484X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260482X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions bovin Bos taurus (Bovine) Bovine anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260481X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. Only protein-protein interactions bovin Bos taurus (Bovine) Bovine pull down pull down anti tag western anti tag western blot anti tag western Sorriento D., Ciccarelli M., Santulli G., Campanile A., Altobelli GG., Cimini V., Galasso G., Astone D., Piscione F., Pastore L., Trimarco B., Iaccarino G. DIP original experiment: DIP-260483X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx The G-protein-coupled receptor kinase 5 inhibits NFkappaB transcriptional activity by inducing nuclear accumulation of IkappaB alpha. ikka_bovin Inhibitor of nuclear factor kappa-B kinase subunit alpha Nuclear factor NF-kappa-B inhibitor kinase alpha CHUK IKKA I-kappa-B kinase 1 protein protein bovin Bos taurus (Bovine) Bovine MERPPGLRPGAGGPWEMRERLGTGGFGNVCLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNSLCSLIVEPMENWLQLMLNWDPQQRGGPVDLTLKQPRCFVLMDHILNLKIVHILNMTSAKIISFLLPPDESLHSLQSRIERETGINTGSQELLSEMGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPIIQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNTNLTKMKNTLISASQQLKAKLEFFHKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYAEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPSDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEMALSNIKEADSTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGVTPQLPPTSAEREHPLSCVVTPQDGETLAQMIEENLNCLGHLSTIIHEANEKQGNNMMSLDWSWLTE DIP protein Q95KV1 original sequence version: 85 mint Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines 01903BE11F44D176 grk5_human G protein-coupled receptor kinase 5 GRK5 GPRK5 G protein-coupled receptor kinase GRK5 protein protein human Homo sapiens Human MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHISQCEDLRRTIDRDYCSLCDKQPIGRLLFRQFCETRPGLECYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFIAQVGQDLVSQTEEKLLQKPCKELFSACAQSVHEYLRGEPFHEYLDSMFFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSQFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHRENTVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVSIPWQNEMIETECFKELNVFGPNGTLPPDLNRNHPPEPPKKGLLQRLFKRQHQNNSKSSPSSKTSFNHHINSNHVSSNSTGSS DIP protein P34947 original sequence version: 136 mint Specifically phosphorylates the activated forms of G protein-coupled receptors D363567ECFF5CF21 arbk1_human Beta-adrenergic receptor kinase 1 GRK2 ADRBK1 BARK BARK1 G-protein coupled receptor kinase 2 protein protein human Homo sapiens Human MADLEAVLADVSYLMAMEKSKATPAARASKKILLPEPSIRSVMQKYLEDRGEVTFEKIFSQKLGYLLFRDFCLNHLEEARPLVEFYEEIKKYEKLETEEERVARSREIFDSYIMKELLACSHPFSKSATEHVQGHLGKKQVPPDLFQPYIEEICQNLRGDVFQKFIESDKFTRFCQWKNVELNIHLTMNDFSVHRIIGRGGFGEVYGCRKADTGKMYAMKCLDKKRIKMKQGETLALNERIMLSLVSTGDCPFIVCMSYAFHTPDKLSFILDLMNGGDLHYHLSQHGVFSEADMRFYAAEIILGLEHMHNRFVVYRDLKPANILLDEHGHVRISDLGLACDFSKKKPHASVGTHGYMAPEVLQKGVAYDSSADWFSLGCMLFKLLRGHSPFRQHKTKDKHEIDRMTLTMAVELPDSFSPELRSLLEGLLQRDVNRRLGCLGRGAQEVKESPFFRSLDWQMVFLQKYPPPLIPPRGEVNAADAFDIGSFDEEDTKGIKLLDSDQELYRNFPLTISERWQQEVAETVFDTINAETDRLEARKKAKNKQLGHEEDYALGKDCIMHGYMSKMGNPFLTQWQRRYFYLFPNRLEWRGEGEAPQSLLTMEEIQSVEETQIKERKCLLLKIRGGKQFILQCDSDPELVQWKKELRDAYREAQQLVQRVPKMKNKPRSPVVELSKVPLVQRGSANGL DIP protein P25098 original sequence version: 156 DIP protein P25098 original sequence version: 124 4DB68F62841AFA7D ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 grk5_bovin G protein-coupled receptor kinase 5 GRK5 GPRK5 G protein-coupled receptor kinase GRK5 protein protein bovin Bos taurus (Bovine) Bovine MELENIVANTVLLKAREGGGGKRKGKSKKWKEILKFPHINQCEDLRRTIDRDYCSLCDKQPVGRLLFRQFCETRPGLESYIQFLDSVAEYEVTPDEKLGEKGKEIMTKYLTPKSPVFITQVGRDLVSQTEEKLLQKPCKELFSACVQSVHDYLRGEPFHEYLDSMYFDRFLQWKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKRLEKKRIKKRKGESMALNEKQILEKVNSRFVVNLAYAYETKDALCLVLTIMNGGDLKFHIYNMGNPGFEEERALFYAAEILCGLEDLHHENIVYRDLKPENILLDDYGHIRISDLGLAVKIPEGDLIRGRVGTVGYMAPEVLNNQRYGLSPDYWGLGCLIYEMIEGQSPFRGRKEKVKREEVDRRVLETEEVYSHKFSEEAKSICKMLLTKDAKQRLGCQEEGAAEVKRHPFFRNMNFKRLEAGMLDPPFVPDPRAVYCKDVLDIEQFSTVKGVNLDHTDDDFYSKFSTGSVPIPWQSEMIETECFKELNVFGPHGTLSPDLNRSHPPEPPKKGLLQRLFKRQHQNNSKSSPNSKTSFNHHINSNHVSSNSTGSS DIP protein P43249 original sequence version: 100 grk5-nfkbia-1 2 9 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction chuk-grk5-1 6 8 unspecified role unspecified role bait bait bovin Bos taurus (Bovine) Bovine 12 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine physical association physical association grk2-nfkbia-1 4 10 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction grk2-nfkbia-2 5 10 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction grk5-nfkbia-2 3 9 unspecified role unspecified role bait bait ecolx Escherichia coli 11 unspecified role unspecified role prey prey spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm direct interaction direct interaction nfkbia-grk5-1 1 11 unspecified role unspecified role bait bait Region 1-58 Region 1-58 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain bovin Bos taurus (Bovine) Bovine 12 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine physical association physical association grk5-chuk-1 7 9 unspecified role unspecified role bait bait Region 50-176 Region 50-176 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range bovin Bos taurus (Bovine) Bovine 8 unspecified role unspecified role prey prey bovin Bos taurus (Bovine) Bovine physical association physical association