DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. PLoS biology 2008 imex curation Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip predetermined predetermined participant predetermined Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. DIP original experiment: DIP-113253X PLoS biology 2008 IMEx The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. PLoS biology 2008 imex curation Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. DIP original experiment: DIP-113256X PLoS biology 2008 IMEx The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. PLoS biology 2008 imex curation Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. DIP original experiment: DIP-113255X PLoS biology 2008 IMEx The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. PLoS biology 2008 imex curation Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. DIP original experiment: DIP-113252X PLoS biology 2008 IMEx The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. PLoS biology 2008 imex curation Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly pull down pull down western blot western blot Immuno blot Immuno blot Sala A., La Rocca G., Burgio G., Kotova E., Di Gesù D., Collesano M., Ingrassia AM., Tulin AV., Corona DF. DIP original experiment: DIP-113254X PLoS biology 2008 IMEx The nucleosome-remodeling ATPase ISWI is regulated by poly-ADP-ribosylation. parp1_human Poly [ADP-ribose] polymerase 1 PARP1 ADPRT PPOL NAD(+) ADP-ribosyltransferase 1 Poly[ADP-ribose] synthase 1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein human Homo sapiens Human MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW DIP protein P09874 original sequence version: 189 DIP protein P09874 original sequence version: 183 DIP protein P09874 original sequence version: 166 DIP protein P09874 original sequence version: 117 DIP protein P09874 original sequence version: 143 DIP protein P09874 original sequence version: 136 DIP protein P09874 original sequence version: 137 DIP protein P09874 original sequence version: 147 DIP protein P09874 original sequence version: 193 DIP protein P09874 original sequence version: 202 DIP protein P09874 original sequence version: 171 DIP protein P09874 original sequence version: 153 DIP protein P09874 original sequence version: 175 6A5FC01EB91C046B iswi_drome Chromatin-remodeling complex ATPase chain Iswi Iswi CG8625 Protein imitation swi Nucleosome-remodeling factor 140 kDa subunit CHRAC 140 kDa subunit protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKTDTAAVEATEENSNETTSDAATSSSGEKEAEFDNKIEADRSRRFDFLLKQTEIFTHFMTNSAKSPTKPKGRPKKIKDKDKEKDVADHRHRKTEQEEDEELLAEDSATKEIFRFDASPAYIKSGEMRDYQIRGLNWMISLYENGINGILADEMGLGKTLQTISLLGYLKHFKNQAGPHIVIVPKSTLQNWVNEFKKWCPSLRAVCLIGDQDTRNTFIRDVLMPGEWDVCVTSYEMCIREKSVFKKFNWRYLVIDEAHRIKNEKSKLSEILREFKTANRLLITGTPLQNNLHELWALLNFLLPDVFNSSEDFDEWFNTNTCLGDDALITRLHAVLKPFLLRRLKAEVEKRLKPKKEMKIFVGLSKMQRDWYTKVLLKDIDVVNGAGKVEKMRLQNILMQLRKCTNHPYLFDGAEPGPPYTTDTHLVYNSGKMAILDKLLPKLQEQGSRVLIFSQMTRMLDILEDYCHWRNYNYCRLDGQTPHEDRNRQIQEFNMDNSAKFLFMLSTRAGGLGINLATADVVIIYDSDWNPQMDLQAMDRAHRIGQKKQVRVFRLITESTVEEKIVERAEVKLRLDKMVIQGGRLVDNRSNQLNKDEMLNIIRFGANQVFSSKETDITDEDIDVILERGEAKTAEQKAALDSLGESSLRTFTMDTNGEAGTSSVYQFEGEDWREKQKLNALGNWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPIVQDFQFFPPRLFELLDQEIYYFRKTVGYKVPKNTELGSDATKVQREEQRKIDEAEPLTEEEIQEKENLLSQGFTAWTKRDFNQFIKANEKYGRDDIDNIAKDVEGKTPEEVIEYNAVFWERCTELQDIERIMGQIERGEGKIQRRLSIKKALDQKMSRYRAPFHQLRLQYGNNKGKNYTEIEDRFLVCMLHKLGFDKENVYEELRAAIRASPQFRFDWFIKSRTALELQRRCNTLITLIERENIELEEKERAEKKKKAPKGSVSAGSGSASSNTPAPAPQPKASQKRKSEVVATSSNSKKKKK DIP protein Q24368 original sequence version: 91 008FC81AE15E071F parp_drome Poly [ADP-ribose] polymerase Parp Poly[ADP-ribose] synthase NAD(+) ADP-ribosyltransferase Protein ADP-ribosyltransferase Parp CG40411 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDIELPYLAEYARTGRATCKGCKSTISKDTLRIAVMVQSAFHDAKVPNWFHKTCFFKNQRPSSVGDIQNIGNLRFADQKELTDLVENIQEVISAQLGKKRSKAFNLALKDFGIEYAKSSRSTCRGCEQKINKDLVRLRKTVYDTEVGMKYGGQPLWHHLECFAQLRSELGWFASGEDMPGFQSLADDDQAKVKNAIPPIKSEELPDTKRAKMELSDTNEEGEKKQRLKDQNDAYFRFRDDIKNKMKKKDIDILLKFNNQQPVTGDTEKLFDQTADLLTFGAIESCSECNSCQFIVNKSGYICNGNHSEWTKCNKLLKEPTRSACIVPKELKALYNFLNTVKEIPSTRIFNNFPPNKSTFSRSLLKTNKNNDVLVRPTIPRISPPLYNLKFSIIGLKNQHKELRKRIENLGGKFEVKISENTIAIISTELEIQKKSTRMKFAEELGIHIVPIEFLDFVEADTEGAIKYINSTCICSWGTDPKSRIPKETTKSLNSNSIYTKSMPVSRTFKVKDGLAVDPDSGLEDIAHVYVDSNNKYSVVLGLTDIQRNKNSYYKVQLLKADKKEKYWIFRSWGRIGTNIGNSKLEEFDTSESAKRNFKEIYADKTGNEYEQRDNFVKRTGRMYPIEIQYDDDQKLVKHESHFFTSKLEISVQNLIKLIFDIDSMNKTLMEFHIDMDKMPLGKLSAHQIQSAYRVVKEIYNVLECGSNTAKLIDATNRFYTLIPHNFGVQLPTLIETHQQIEDLRQMLDSLAEIEVAYSIIKSEDVSDACNPLDNHYAQIKTQLVALDKNSEEFSILSQYVKNTHASTHKSYDLKIVDVFKVSRQGEARRFKPFKKLHNRKLLWHGSRLTNFVGILSHGLRIAPPEAPPTGYMFGKGIYFADMVSKSANYCCTSQQNSTGLMLLSEVALGDMMECTSAKYINKLSNNKHSCFGRGRTMPDPTKSYIRSDGVEIPYGETITDEHLKSSLLYNEYIVYDVAQVNIQYLFRMEFKYSY DIP protein P35875 original sequence version: 99 ACA85A270DD29E08 iswi-parp-1 4 7 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association iswi-parp-2 3 7 unspecified role unspecified role bait bait CBP-tag CBP-tag tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association iswi-parp1-1 1 6 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction parp-iswi-1 2 7 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role bait bait drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association iswi-parp-3 5 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 8 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association