DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions in vitro In vitro protein kinase assay protein kinase assay predetermined predetermined participant predetermined Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113538X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-264631X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113537X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-264630X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113540X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113539X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113535X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. Nature structural & molecular biology 2008 imex curation Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Shirakawa K., Takaori-Kondo A., Yokoyama M., Izumi T., Matsui M., Io K., Sato T., Sato H., Uchiyama T. DIP original experiment: DIP-113536X Nature structural & molecular biology 2008 IMEx Phosphorylation of APOBEC3G by protein kinase A regulates its interaction with HIV-1 Vif. ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG abc3g_human DNA dC->dU-editing enzyme APOBEC-3G APOBEC-related cytidine deaminase APOBEC-related protein 9 CEM-15 APOBEC3G Deoxycytidine deaminase MDS019 protein protein human Homo sapiens Human MKPHFRNTVERMYRDTFSYNFYNRPILSRRNTVWLCYEVKTKGPSRPPLDAKIFRGQVYSELKYHPEMRFFHWFSKWRKLHRDQEYEVTWYISWSPCTKCTRDMATFLAEDPKVTLTIFVARLYYFWDPDYQEALRSLCQKRDGPRATMKIMNYDEFQHCWSKFVYSQRELFEPWNNLPKYYILLHIMLGEILRHSMDPPTFTFNFNNEPWVRGRHETYLCYEVERMHNDTWVLLNQRRGFLCNQAPHKHGFLEGRHAELCFLDVIPFWKLDLDQDYRVTCFTSWSPCFSCAQEMAKFISKNKHVSLCIFTARIYDDQGRCQEGLRTLAEAGAKISIMTYSEFKHCWDTFVDHQGCPFQPWDGLDEHSQDLSGRLRAILQNQEN DIP protein Q9HC16 original sequence version: 140 DIP protein Q9HC16 original sequence version: 68 DIP protein Q9HC16-1 original sequence version: 88 DIP protein Q9HC16-1 original sequence version: 84 DIP protein Q9HC16 original sequence version: 138 DIP protein Q9HC16-1 original sequence version: 68 60525DC3B7D903D6 kapca_human cAMP-dependent protein kinase catalytic subunit alpha PRKACA PKACA protein protein human Homo sapiens Human MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF DIP protein P17612 original sequence version: 171 DIP protein P17612 original sequence version: 175 DIP protein P17612 original sequence version: 178 DIP protein P17612-1 original sequence version: 177 DIP protein P17612 original sequence version: 186 DIP protein P17612 original sequence version: 102 DIP protein P17612-1 original sequence version: 102 BF6D3ECD2614E5AB q77yg0_9hiv1 Virion infectivity factor vif SOR protein protein protein 9hiv1 Human immunodeficiency virus 1 MENRWQVMIVWQVDRMRIRTWKSLVKHHMYVSGKARGWFYRHHYESPHPRISSEVHIPLGDARLVITTYWGLHTGERDWHLGQGVSIEWRKKRYSTQVDPELADQLIHLYYFDCFSDSAIRKALLGHIVSPRCEYQAGHNKVGSLQYLALAALITPKKIKPPLPSVTKLTEDRWNKPQKTKGHRGSHTMNGH DIP protein Q77YG0 original sequence version: 49 DIP protein Q77YG0 original sequence version: 39 apobec3g-prkaca-1 7 11 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association apobec3g-prkaca-2 3 11 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association apobec3g-vif-5 5 12 unspecified role unspecified role prey prey human Homo sapiens Human 10 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q9HC16:p.Thr32Asp T32D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T32D'. Wed May 02 19:22:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr32asp' Tue Jun 30 16:35:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr32Asp' Q9HC16:p.[Thr32Asp;Thr218Asp] T32D/T218D mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T D certain certain sequence position certain certain certain sequence position certain T D This feature has been corrected as a result of our quality control procedures. The original label was 'T32D/T218D'. Wed May 02 16:57:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr32asp,thr218asp' Tue Jun 30 16:00:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Thr32Asp;Thr218Asp]' human Homo sapiens Human physical association physical association apobec3g-prkaca-3 8 11 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association apobec3g-prkaca-4 1 11 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 10 enzyme target enzyme target substrate substrate unspecified role unspecified role spofr Spodoptera frugiperda (Fall armyworm) Fall armyworm phosphorylation phosphorylation reaction phosphorylation apobec3g-ub-1 6 9 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal human Homo sapiens Human 10 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association apobec3g-prkaca-5 4 11 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role bait bait HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association 1 nodeMerge04 2015-04-26 apobec3g-prkaca-6 2 11 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 10 unspecified role unspecified role bait bait GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association 1 nodeMerge04 2015-04-26