DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 2008 imex curation Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. DIP original experiment: DIP-250227X Nature 2008 IMEx E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 2008 imex curation Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. DIP original experiment: DIP-250229X Nature 2008 IMEx E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 2008 imex curation Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. DIP original experiment: DIP-250228X Nature 2008 IMEx E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 2008 imex curation Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. DIP original experiment: DIP-250230X Nature 2008 IMEx E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. Nature 2008 imex curation Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. Only protein-protein interactions human Homo sapiens Human anti bait coip anti bait coimmunoprecipitation anti bait coip predetermined predetermined participant predetermined Morris EJ., Ji JY., Yang F., Di Stefano L., Herr A., Moon NS., Kwon EJ., Haigis KM., Näär AM., Dyson NJ. DIP original experiment: DIP-250231X Nature 2008 IMEx E2F1 represses beta-catenin transcription and is antagonized by both pRB and CDK8. cdk8_human Cyclin-dependent kinase 8 CDK8 Cell division protein kinase 8 Mediator of RNA polymerase II transcription subunit CDK8 Mediator complex subunit CDK8 Protein kinase K35 protein protein human Homo sapiens Human MDYDFKVKLSSERERVEDLFEYEGCKVGRGTYGHVYKAKRKDGKDDKDYALKQIEGTGISMSACREIALLRELKHPNVISLQKVFLSHADRKVWLLFDYAEHDLWHIIKFHRASKANKKPVQLPRGMVKSLLYQILDGIHYLHANWVLHRDLKPANILVMGEGPERGRVKIADMGFARLFNSPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPADKDWEDIKKMPEHSTLMKDFRRNTYTNCSLIKYMEKHKVKPDSKAFHLLQKLLTMDPIKRITSEQAMQDPYFLEDPLPTSDVFAGCQIPYPKREFLTEEEPDDKGDKKNQQQQQGNNHTNGTGHPGNQDSSHTQGPPLKKVRVVPPTTTSGGLIMTSDYQRSNPHAAYPNPGPSTSQPQSSMGYSATSQQPPQYSHQTHRY DIP protein P49336 original sequence version: 126 DIP protein P49336 original sequence version: 115 DIP protein P49336 original sequence version: 89 DIP protein P49336-1 original sequence version: 149 DIP protein P49336 original sequence version: 138 00CBAB6CE354605F e2f1_human Transcription factor E2F1 Retinoblastoma-associated protein 1 Retinoblastoma-binding protein 3 PBR3 E2F1 RBBP3 pRB-binding protein E2F-1 protein protein human Homo sapiens Human MALAGAPAGGPCAPALEALLGAGALRLLDSSQIVIISAAQDASAPPAPTGPAAPAAGPCDPDLLLFATPQAPRPTPSAPRPALGRPPVKRRLDLETDHQYLAESSGPARGRGRHPGKGVKSPGEKSRYETSLNLTTKRFLELLSHSADGVVDLNWAAEVLKVQKRRIYDITNVLEGIQLIAKKSKNHIQWLGSHTTVGVGGRLEGLTQDLRQLQESEQQLDHLMNICTTQLRLLSEDTDSQRLAYVTCQDLRSIADPAEQMVMVIKAPPETQLQAVDSSENFQISLKSKQGPIDVFLCPEETVGGISPGKTPSQEVTSEEENRATDSATIVSPPPSSPPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF DIP protein Q01094 original sequence version: 173 DIP protein Q01094 original sequence version: 168 DIP protein Q01094 original sequence version: 149 DIP protein Q01094 original sequence version: 130 DIP protein Q01094 original sequence version: 125 DIP protein Q01094 original sequence version: 153 DIP protein Q01094 original sequence version: 180 003B3F654F0C60DF e2f1_drome Transcription factor E2f1 E2f1 E2f dE2F CG6376 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSKFFVNVAPINNSNSSSSHTTTSSNTQRHQQHQQHYGGSGTTGHTMVARRLNYDLHGGTTSINNNNNIVIKNESVDLDYDHVLSSSDSNSNGGVAAHLRDHVYISLDKGHNTGAVATAAAAATAGHTQQQLQQQHHHQNQQQRKATGKSNDITNYYKVKRRPHAVSDEIHPKKQAKQSAHHQTVYQKHTASSAPQQLRHSHHQLRHDADAELDEDVVERVAKPASHHPFSLSTPQQQLAASVASSSSSGDRNRADTSLGILTKKFVDLLQESPDGVVDLNEASNRLHVQKRRIYDITNVLEGINILEKKSKNNIQWRCGQSMVSQERSRHIEADSLRLEQQENELNKAIDLMRENLAEISQEVENSGGMAYVTQNDLLNVDLFKDQIVIVIKAPPEAKLVLPNTKLPREIYVKAENSGEINVFLCHDTSPENSPIAPGAGYVGAPGAGCVRTATSTRLHPLTNQRLNDPLFNNIDAMSTKGLFQTPYRSARNLSKSIEEAAKQSQPEYNNICDIAMGQHHNLNQQQQQQQQQLLQQPEEDDVDVELNQLVPTLTNPVVRTHQFQQHQQPSIQELFSSLTESSPPTPTKRRREAAAAAIAAGSSTTATTTLNSHNNRNHSNHSNHSNHSSSNNSKSQPPTIGYGSSQRRSDVPMYNCAMEGATTTSATADTTAATSRSAAASSLQMQFAAVAESNNGSSSGGGGGGGGYGSIAGAGANADPHQPYSHDRNSLPPGVADCDANSNSSSVTLQGLDALFNDIGSDYFSNDIAFVSINPPDDNDYPYALNANEGIDRLFDFGSDAYGP DIP protein Q27368 original sequence version: 121 DIP protein Q27368 original sequence version: 107 BD49C813DDB2A77D cdk8_drome Cyclin-dependent kinase 8 Cdk8 CG10572 Cell division protein kinase 8 Mediator of RNA polymerase II transcription subunit Cdk8 Mediator complex subunit Cdk8 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDYDFKMKTQIERTKVEDLFNYEGCKVGRGTYGHVYKAKWKETSDGKEYALKQIDGTGLSMSACREIALLRELKHQNVITLIRVFLSHNDRKVFLLIDYAEHDLWHIIKFHRAAKATKKQVVVPRGMVKSLLYQILDGIHYLHSNWVLHRDLKPANILVMGDGNERGRVKIADMGFARLFNAPLKPLADLDPVVVTFWYRAPELLLGARHYTKAIDIWAIGCIFAELLTSEPIFHCRQEDIKTSNPYHHDQLDRIFNVMGFPQDKDWEDIKKMPEHHTLTKDFKRSTYSTCSLAKYMERHKIKPDSKAFHLLQKLLLMDPNKRITSEQAMQDQYFQEEPQPTQDVFAGCPIPYPKREFLTDDDQEDKSDNKRQQQQQQQQQQQQQQQQQQQQQQQQQQQQMNAEPNAKRVRLSGAGNQQDFHHQQQQQQQQQQQQQQQQQQMMFNQQQNFQRFN DIP protein Q9VT57 original sequence version: 95 D73F6219E2A03C98 e2f1-cdk8-1 1 6 unspecified role unspecified role prey prey human Homo sapiens Human 7 unspecified role unspecified role bait bait human Homo sapiens Human physical association physical association cdk8-e2f1-1 4 9 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 8 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association cdk8-e2f1-2 3 6 unspecified role unspecified role bait bait human Homo sapiens Human 7 unspecified role unspecified role prey prey human Homo sapiens Human physical association physical association e2f1-cdk8-2 2 9 unspecified role unspecified role prey prey HA-tag HA-tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human 8 unspecified role unspecified role bait bait Region 561-769 Region 561-769 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Region 733-805 Region 733-805 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association cdk8-e2f1-3 5 6 unspecified role unspecified role bait bait human Homo sapiens Human 7 unspecified role unspecified role prey prey p-residue p-residue phosres phosphorylated residue PhosRes Phospho Phosphorylation Phosphorylation (O of Serine, Threonine, Tyrosine and Aspartate, N epsilon of Lysine) undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined human Homo sapiens Human physical association physical association