DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250191X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250189X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250198X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250197X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250190X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250195X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250192X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250193X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250194X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. Nature 2008 imex curation McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie McLellan JS., Zheng X., Hauk G., Ghirlando R., Beachy PA., Leahy DJ. DIP original experiment: DIP-250196X Nature 2008 IMEx The mode of Hedgehog binding to Ihog homologues is not conserved across different phyla. shh_mouse Sonic hedgehog protein HHG-1 Shh Hhg1 Shh unprocessed N-terminal signaling and C-terminal autoprocessing domains protein protein mouse Mus musculus Mouse MLLLLARCFLVILASSLLVCPGLACGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGGCFPGSATVHLEQGGTKLVKDLRPGDRVLAADDQGRLLYSDFLTFLDRDEGAKKVFYVIETLEPRERLLLTAAHLLFVAPHNDSGPTPGPSALFASRVRPGQRVYVVAERGGDRRLLPAAVHSVTLREEEAGAYAPLTAHGTILINRVLASCYAVIEEHSWAHRAFAPFRLAHALLAALAPARTDGGGGGSIPAAQSATEARGAEPTAGIHWYSQLLYHIGTWLLDSETMHPLGMAVKSS DIP protein Q62226 original sequence version: 148 DIP protein Q62226 original sequence version: 140 DIP protein Q62226 original sequence version: 118 q02936-1 Protein hedgehog hh CG4637 A B protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MDNHSSVPWASAASVTCLSLDAKCHSSSSSSSSKSAASSISAIPQEETQTMRHIAHTQRCLSRLTSLVALLLIVLPMVFSPAHSCGPGRGLGRHRARNLYPLVLKQTIPNLSEYTNSASGPLEGVIRRDSPKFKDLVPNYNRDILFRDEEGTGADRLMSKRCKEKLNVLAYSVMNEWPGIRLLVTESWDEDYHHGQESLHYEGRAVTIATSDRDQSKYGMLARLAVEAGFDWVSYVSRRHIYCSVKSDSSISSHVHGCFTPESTALLESGVRKPLGELSIGDRVLSMTANGQAVYSEVILFMDRNLEQMQNFVQLHTDGGAVLTVTPAHLVSVWQPESQKLTFVFADRIEEKNQVLVRDVETGELRPQRVVKVGSVRSKGVVAPLTREGTIVVNSVAASCYAVINSQSLAHWGLAPMRLLSTLEAWLPAKEQLHSSPKVVSSAQQQNGIHWYANALYKVKDYVLPQSWRHD DIP protein Q02936-1 original sequence version: 167 DIP protein Q02936 original sequence version: 115 DIP protein Q02936-1 original sequence version: 136 DIP protein Q02936-1 original sequence version: 145 gas1_mouse Growth arrest-specific protein 1 Gas1 Gas-1 protein protein mouse Mus musculus Mouse MLAALLGGAGARTGTLPGALLCLMALLQLLCSAPRGSGLAHGRRLICWQALLQCQGEPDCSYAYSQYAEACAPVLAQRGGADAPGPAGAFPASAASSPRWRCPSHCISALIQLNHTRRGPALEDCDCAQDEHCRSTKRAIEPCLPRTSSVGPGAGAGSVMGCTEARRRCDRDSRCNLALSRYLAYCGKLFNGLRCTDECRAVIEDMLAVPKAALLNDCVCDGLERPICESVKENMARLCFGPDASNGPGSSGSDGGLDDYYDEEYDDEQRAGAAGGEQPLDDDDGLARPGGGAAAAGGRGDLPHGPGRRSSSSGSGGHWANRSAWTPFACLLLLLLLLLGSHL DIP protein Q01721 original sequence version: 88 ihh_mouse Indian hedgehog protein Ihh HHG-2 protein protein mouse Mus musculus Mouse MSPAWLRPRLRFCLFLLLLLLVPAARGCGPGRVVGSRRRPPRKLVPLAYKQFSPNVPEKTLGASGRYEGKIARSSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDRLNSLAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRNKYGLLARLAVEAGFDWVYYESKAHVHCSVKSEHSAAAKTGGCFPAGAQVRLENGERVALSAVKPGDRVLAMGEDGTPTFSDVLIFLDREPNRLRAFQVIETQDPPRRLALTPAHLLFIADNHTEPAAHFRATFASHVQPGQYVLVSGVPGLQPARVAAVSTHVALGSYAPLTRHGTLVVEDVVASCFAAVADHHLAQLAFWPLRLFPSLAWGSWTPSEGVHWYPQMLYRLGRLLLEESTFHPLGMSGAGS DIP protein P97812 original sequence version: null cdon_human Cell adhesion molecule-related/down-regulated by oncogenes CDON CDO protein protein human Homo sapiens Human MHPDLGPLCTLLYVTLTILCSSVSSDLAPYFTSEPLSAVQKLGGPVVLHCSAQPVTTRISWLHNGKTLDGNLEHVKIHQGTLTILSLNSSLLGYYQCLANNSIGAIVSGPATVSVAVLGDFGSSTKHVITAEEKSAGFIGCRVPESNPKAEVRYKIRGKWLEHSTENYLILPSGNLQILNVSLEDKGSYKCAAYNPVTHQLKVEPIGRKLLVSRPSSDDVHILHPTHSQALAVLSRSPVTLECVVSGVPAPQVYWLKDGQDIAPGSNWRRLYSHLATDSVDPADSGNYSCMAGNKSGDVKYVTYMVNVLEHASISKGLQDQIVSLGATVHFTCDVHGNPAPNCTWFHNAQPIHPSARHLTAGNGLKISGVTVEDVGMYQCVADNGIGFMHSTGRLEIENDGGFKPVIITAPVSAKVADGDFVTLSCNASGLPVPVIRWYDSHGLITSHPSQVLRSKSRKSQLSRPEGLNLEPVYFVLSQAGASSLHIQAVTQEHAGKYICEAANEHGTTQAEASLMVVPFETNTKAETVTLPDAAQNDDRSKRDGSETGLLSSFPVKVHPSAVESAPEKNASGISVPDAPIILSPPQTHTPDTYNLVWRAGKDGGLPINAYFVKYRKLDDGVGMLGSWHTVRVPGSENELHLAELEPSSLYEVLMVARSAAGEGQPAMLTFRTSKEKTASSKNTQASSPPVGIPKYPVVSEAANNNFGVVLTDSSRHSGVPEAPDRPTISTASETSVYVTWIPRANGGSPITAFKVEYKRMRTSNWLVAAEDIPPSKLSVEVRSLEPGSTYKFRVIAINHYGESFRSSASRPYQVVGFPNRFSSRPITGPHIAYTEAVSDTQIMLKWTYIPSSNNNTPIQGFYIYYRPTDSDNDSDYKRDVVEGSKQWHMIGHLQPETSYDIKMQCFNEGGESEFSNVMICETKVKRVPGASEYPVKDLSTPPNSLGSGGNVGPATSPARSSDMLYLIVGCVLGVMVLILMVFIAMCLWKNRQQNTIQKYDPPGYLYQGSDMNGQMVDYTTLSGASQINGNVHGGFLTNGGLSSGYSHLHHKVPNAVNGIVNGSLNGGLYSGHSNSLTRTHVDFEHPHHLVNGGGMYTAVPQIDPLECVNCRNCRNNNRCFTKTNSTFSSSPPPVVPVVAPYPQDGLEMKPLSHVKVPVCLTSAVPDCGQLPEESVKDNVEPVPTQRTCCQDIVNDVSSDGSEDPAEFSRGQEGMINLRIPDHLQLAKSCVWEGDSCAHSETEINIVSWNALILPPVPEGCAEKTMWSPPGIPLDSPTEVLQQPRET DIP protein Q4KMG0 original sequence version: 49 mint Component of a cell-surface receptor complex that mediates cell-cell interactions between muscle precursor cells. Promotes differentiation of myogenic cells B7870C66F5224BBE ihog_drome Interference hedgehog ihog CG9211 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTLLTSSLLLFSLLTSRLEAIPVLEKSPAHPAHSAHPAHPAHPAHPAHPSPGVRILRAPESLVAPLGDEVVLECETSLQPERFEWSHRSSRSPGAGFKYLKTGTAKANVSQEAAISRLRVLVRPDTLGEYRCVGWFGPLVVTSTIARLELASTSLVDAQESESPLQWRVSAGNSVLWSCGQQVQSNPSASWSYYRNGVEIKPEFIGTNGNLFLSNVSSESSGSYSCQATNPASGERIQLPGSLQLQVTPEQRSESKSPHLLRGQPSSQEITIREGSSLLLLCPGVGSPPPTVVWSSPDVVGAVKNKRSKVFGHALEISNTRVNDAGTYICFQDNGVRPALEHYIKVHVEQPPQIVRPPWADLTNEGDRLKLECKATGVPTPEIYWLLNGHSSIDDSEAELSNNFLILHSVLKRHAGYVQCFARNRLGEHSAGTLLQVNPKQIQEPRESGGTHRPKPNQGSRQKQMYPPTPPNVTRLSDESVMLRWMVPRNDGLPIVIFKVQYRMVGKRKNWQTTNDNIPYGKPKWNSELGKSFTASVTDLKPQHTYRFRILAVYSNNDNKESNTSAKFYLQPGAALDPMPVPELLEIEEYSETAVVLHWSLASDADEHLITGYYAYYRPSSSAGEYFKATIEGAHARSFKIAPLETATMYEFKLQSFSAASASEFSALKQGRTQRPKTSTTEEPTLQMGDRDTTTPSHNETFNMSPMLTGTIGGGAVLILLLISTCFCVCRRRNSRSRGNNPNKPRMAELRDDFVPLGNCSPTKQRQRTRHIHITLNPLAQQQQQALEEKNDTDQDAPYYQRPSSYDYDPTLRRMSSSSLRRSQRTLERAGGSNGSNNGNNNNLNQSAEAGSIENPGKPGRVLMKRPRLSSRSENLSSGSLNSVGV DIP protein Q9VM64 original sequence version: 125 DIP protein Q9VM64 original sequence version: 102 DIP protein Q9VM64 original sequence version: 93 DIP protein Q9VM64 original sequence version: 101 8DA038FA9107E227 cdon-shh-2 1 15 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ihog-shh-1 8 16 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cdon-shh-3 4 15 unspecified role unspecified role bait bait Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D V This feature has been corrected as a result of our quality control procedures. The original label was 'D89V'. Wed May 02 15:58:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp89val' Tue Jun 30 19:10:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp89Val' Q62226:p.Glu127Lys E127K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E127K'. Wed May 02 20:03:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu127lys' Tue Jun 30 19:15:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu127Lys' Q62226:p.[Glu90Gln;Glu91Gln;Glu127Gln;Asp132Asn] E90Q/E91Q/E127Q/D132N mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E Q certain certain sequence position certain certain certain sequence position certain E Q certain certain sequence position certain certain certain sequence position certain D N certain certain sequence position certain certain certain sequence position certain E Q This feature has been corrected as a result of our quality control procedures. The original label was 'E90Q/E91Q/E127Q/D132N'. Wed May 02 15:11:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu90gln,glu91gln,glu127gln,asp132asn' Tue Jun 30 15:38:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu90Gln;Glu91Gln;Glu127Gln;Asp132Asn]' Q62226:p.[Lys88Ala;Arg124Ala;Arg154Ala;Ser178Ala] K88A/R124A/R154A/S178A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain R A certain certain sequence position certain certain certain sequence position certain R A This feature has been corrected as a result of our quality control procedures. The original label was 'K88A/R124A/R154A/S178A'. Wed May 02 16:54:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys88ala,arg124ala,arg154ala,ser178ala' Tue Jun 30 18:18:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys88Ala;Arg124Ala;Arg154Ala;Ser178Ala]' ecolx Escherichia coli direct interaction direct interaction cdon-shh-4 2 15 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction gas1-shh-1 6 13 unspecified role unspecified role bait bait Fc-tag Fc-tag fusion protein fusion protein c-term range C-terminal range c-term range c-term range C-terminal range c-term range chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey 11 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D V This feature has been corrected as a result of our quality control procedures. The original label was 'D89V'. Wed May 02 20:33:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp89val' Tue Jun 30 17:37:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp89Val' Q62226:p.Glu127Lys E127K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E127K'. Wed May 02 17:18:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu127lys' Wed May 02 18:51:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu127lys' Tue Jun 30 20:21:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu127Lys' ecolx Escherichia coli direct interaction direct interaction cdon-ihh-1 3 15 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 14 unspecified role unspecified role unspecified role unspecified role Region 67-231 Region 67-231 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain P97812:p.Glu131Lys E131K mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E K This feature has been corrected as a result of our quality control procedures. The original label was 'E131K'. Wed May 02 14:30:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu131lys' Tue Jun 30 16:36:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu131Lys' ecolx Escherichia coli direct interaction direct interaction cdon-shh-5 7 15 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q62226:p.Asp89Val D89V mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D V This feature has been corrected as a result of our quality control procedures. The original label was 'D89V'. Wed May 02 14:53:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp89val' Tue Jun 30 20:17:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp89Val' ecolx Escherichia coli direct interaction direct interaction hh-ihog-4 9 12 unspecified role unspecified role unspecified role unspecified role Region 86-248 Region 86-248 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 16 unspecified role unspecified role unspecified role unspecified role Region 466-679 Region 466-679 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cdon-shh-6 5 15 unspecified role unspecified role unspecified role unspecified role Region 826-924 Region 826-924 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role unspecified role unspecified role Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cdon-shh-7 10 15 unspecified role unspecified role bait bait Region 722-927 Region 722-927 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 11 unspecified role unspecified role prey prey Region 26-189 Region 26-189 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction