DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. DIP original experiment: DIP-113358X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. Only protein-protein interactions ecolx Escherichia coli sls static light scattering sls predetermined predetermined participant predetermined Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. DIP original experiment: DIP-113357X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. Only protein-protein interactions in vitro In vitro pull down pull down weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. DIP original experiment: DIP-113359X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. DIP original experiment: DIP-113356X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Structural basis for suppression of a host antiviral response by influenza A virus. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. Only protein-protein interactions human Homo sapiens Human comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel western blot western blot Immuno blot Immuno blot Das K., Ma LC., Xiao R., Radvansky B., Aramini J., Zhao L., Marklund J., Kuo RL., Twu KY., Arnold E., Krug RM., Montelione GT. DIP original experiment: DIP-113360X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Structural basis for suppression of a host antiviral response by influenza A virus. o95639-1 Cleavage and polyadenylation specificity factor subunit 4 CPSF4 CPSF30 NAR NEB1 Cleavage and polyadenylation specificity factor 30 kDa subunit NS1 effector domain-binding protein 1 No arches homolog protein protein human Homo sapiens Human MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQSNNPPLQRSSSLIQLTSQNSSPNQQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ DIP protein O95639-1 original sequence version: 63 irf3_human Interferon regulatory factor 3 IRF3 protein protein human Homo sapiens Human MGTPKPRILPWLVSQLDLGQLEGVAWVNKSRTRFRIPWKHGLRQDAQQEDFGIFQAWAEATGAYVPGRDKPDLPTWKRNFRSALNRKEGLRLAEDRSKDPHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGDRTLPGWPVTLPDPGMSLTDRGVMSYVRHVLSCLGGGLALWRAGQWLWAQRLGHCHTYWAVSEELLPNSGHGPDGEVPKDKEGGVFDLGPFIVDLITFTEGSGRSPRYALWFCVGESWPQDQPWTKRLVMVKVVPTCLRALVEMARVGGASSLENTVDLHISNSHPLSLTSDQYKAYLQDLVEGMDFQGPGES DIP protein Q14653 original sequence version: 96 DIP protein Q14653 original sequence version: 75 DIP protein Q14653 original sequence version: 95 DIP protein Q14653-1 original sequence version: 128 DIP protein Q14653 original sequence version: 119 F536676FA78B0110 ns1_i72a2 Non-structural protein 1 NS NS1A protein protein i72a2 Influenza A virus (strain A/Udorn/307/1972 H3N2) MDSNTVSSFQVDCFLWHVRKQVVDQELGDAPFLDRLRRDQKSLRGRGSTLGLNIEAATHVGKQIVEKILKEESDEALKMTMASTPASRYITDMTIEELSRDWFMLMPKQKVEGPLCIRIDQAIMDKNIMLKANFSVIFDRLETLILLRAFTEEGAIVGEISPLPSFPGHTIEDVKNAIGVLIGGLEWNDNTVRVSKTLQRFAWGSSNENGRPPLTPKQKRKMARTARSKVRRDKMAD DIP protein P03495 original sequence version: 109 DIP protein P03495 original sequence version: 73 DIP protein P03495 original sequence version: 88 DIP protein P03495 original sequence version: 101 14D0308795627E5E cpsf4-ns-1 1 8 unspecified role unspecified role unspecified role unspecified role Region 85-215 Region 85-215 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 60-120 Region 60-120 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction cpsf4-ns-2 4 8 unspecified role unspecified role unspecified role unspecified role Region 85-215 Region 85-215 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 60-120 Region 60-120 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction irf3-6 5 7 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human physical association physical association cpsf4-ns-3 3 8 unspecified role unspecified role prey prey P03495:p.Gln121Ala Q121A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Q A This feature has been corrected as a result of our quality control procedures. The original label was 'Q121A'. Wed May 02 16:15:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln121ala' Tue Jun 30 19:50:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln121Ala' P03495:p.Trp187Arg W187R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W R This feature has been corrected as a result of our quality control procedures. The original label was 'W187R'. Wed May 02 18:28:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp187arg' Tue Jun 30 18:39:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp187Arg' P03495:p.Gly184Arg G184R mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain G R This feature has been corrected as a result of our quality control procedures. The original label was 'G184R'. Wed May 02 14:25:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly184arg' Tue Jun 30 16:38:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly184Arg' in vitro In vitro 6 unspecified role unspecified role bait bait Region 60-120 Region 60-120 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction cpsf4-ns-4 2 8 unspecified role unspecified role unspecified role unspecified role Region 85-215 Region 85-215 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 60-120 Region 60-120 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction