DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133222X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds western blot western blot Immuno blot Immuno blot Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133225X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133224X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds autoradiography autoradiography Yamoah K., Oashi T., Sarikas A., Gazdoiu S., Osman R., Pan ZQ. DIP original experiment: DIP-133223X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Autoinhibitory regulation of SCF-mediated ubiquitination by human cullin 1's C-terminal tail. cul1_human Cullin-1 CUL1 protein protein human Homo sapiens Human MSSTRSQNPHGLKQIGLDQIWDDLRAGIQQVYTRQSMAKSRYMELYTHVYNYCTSVHQSNQARGAGVPPSKSKKGQTPGGAQFVGLELYKRLKEFLKNYLTNLLKDGEDLMDESVLKFYTQQWEDYRFSSKVLNGICAYLNRHWVRRECDEGRKGIYEIYSLALVTWRDCLFRPLNKQVTNAVLKLIEKERNGETINTRLISGVVQSYVELGLNEDDAFAKGPTLTVYKESFESQFLADTERFYTRESTEFLQQNPVTEYMKKAEARLLEEQRRVQVYLHESTQDELARKCEQVLIEKHLEIFHTEFQNLLDADKNEDLGRMYNLVSRIQDGLGELKKLLETHIHNQGLAAIEKCGEAALNDPKMYVQTVLDVHKKYNALVMSAFNNDAGFVAALDKACGRFINNNAVTKMAQSSSKSPELLARYCDSLLKKSSKNPEEAELEDTLNQVMVVFKYIEDKDVFQKFYAKMLAKRLVHQNSASDDAEASMISKLKQACGFEYTSKLQRMFQDIGVSKDLNEQFKKHLTNSEPLDLDFSIQVLSSGSWPFQQSCTFALPSELERSYQRFTAFYASRHSGRKLTWLYQLSKGELVTNCFKNRYTLQASTFQMAILLQYNTEDAYTVQQLTDSTQIKMDILAQVLQILLKSKLLVLEDENANVDEVELKPDTLIKLYLGYKNKKLRVNINVPMKTEQKQEQETTHKNIEEDRKLLIQAAIVRIMKMRKVLKHQQLLGEVLTQLSSRFKPRVPVIKKCIDILIEKEYLERVDGEKDTYSYLA DIP protein Q13616 original sequence version: 144 DIP protein Q13616 original sequence version: 133 DIP protein Q13616 original sequence version: null DIP protein Q13616 original sequence version: 135 DIP protein Q13616 original sequence version: 98 DIP protein Q13616 original sequence version: 110 DIP protein Q13616 original sequence version: 146 DIP protein Q13616 original sequence version: 148 DIP protein Q13616 original sequence version: 100 DIP protein Q13616 original sequence version: 85 DIP protein Q13616 original sequence version: 114 DIP protein Q13616 original sequence version: 119 DIP protein Q13616 original sequence version: 136 DIP protein Q13616 original sequence version: 117 DIP protein Q13616 original sequence version: 99 DIP protein Q13616 original sequence version: 108 6625A1FFA7799BBA ikba_human NF-kappa-B inhibitor alpha NFKBIA IKBA MAD3 NFKBI I-kappa-B-alpha Major histocompatibility complex enhancer-binding protein MAD3 protein protein human Homo sapiens Human MFQAAERPQEWAMEGPRDGLKKERLLDDRHDSGLDSMKDEEYEQMVKELQEIRLEPQEVPRGSEPWKQQLTEDGDSFLHLAIIHEEKALTMEVIRQVKGDLAFLNFQNNLQQTPLHLAVITNQPEIAEALLGAGCDPELRDFRGNTPLHLACEQGCLASVGVLTQSCTTPHLHSILKATNYNGHTCLHLASIHGYLGIVELLVSLGADVNAQEPCNGRTALHLAVDLQNPDLVSLLLKCGADVNRVTYQGYSPYQLTWGRPSTRIQQQLGQLTLENLQMLPESEDEESYDTESEFTEFTEDELPYDDCVFGGQRLTL DIP protein P25963 original sequence version: 160 DIP protein P25963 original sequence version: 157 DIP protein P25963 original sequence version: 145 DIP protein P25963 original sequence version: 170 DIP protein P25963 original sequence version: 122 DIP protein P25963 original sequence version: 128 DIP protein P25963 original sequence version: 78 DIP protein P25963 original sequence version: 121 DIP protein P25963 original sequence version: 124 088B313226786395 ub2r1_human Ubiquitin-conjugating enzyme E2 R1 Ubiquitin-protein ligase R1 Ubiquitin-conjugating enzyme E2-32 kDa complementing Ubiquitin-conjugating enzyme E2-CDC34 CDC34 UBE2R1 UBCH3 (E3-independent) E2 ubiquitin-conjugating enzyme R1 E2 ubiquitin-conjugating enzyme R1 protein protein human Homo sapiens Human MARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES DIP protein P49427 original sequence version: 101 DIP protein P49427 original sequence version: 134 258960666B589DB3 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG nedd8_human NEDD8 Ubiquitin-like protein Nedd8 Neddylin Neural precursor cell expressed developmentally down-regulated protein 8 NEDD8 protein protein human Homo sapiens Human MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGGGLRQ DIP protein Q15843 original sequence version: 103 DIP protein Q15843 original sequence version: 149 DIP protein Q15843 original sequence version: 58 DIP protein Q15843 original sequence version: 124 DIP protein Q15843 original sequence version: 95 DIP protein Q15843 original sequence version: 111 DC2FE102BE4725D2 nfkbia-ub-2 1 8 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 6 unspecified role unspecified role unspecified role unspecified role GST tag GST tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli covalent binding covalent binding cul1-nedd8-3 2 9 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 5 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human covalent binding covalent binding cdc34-ub-1 4 8 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human 7 unspecified role unspecified role unspecified role unspecified role human Homo sapiens Human covalent binding covalent binding cul1-nedd8-4 3 9 unspecified role unspecified role prey prey human Homo sapiens Human 5 unspecified role unspecified role bait bait Flag tag Flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q13616:p.[Lys720Arg;Tyr761Ala] K720R/Y761A mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain K R This feature has been corrected as a result of our quality control procedures. The original label was 'K720R/Y761A'. Wed May 02 17:31:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys720arg,tyr761ala' Tue Jun 30 20:14:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys720Arg;Tyr761Ala]' human Homo sapiens Human physical association physical association