DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Zhang Y., Oh H., Burton RA., Burgner JW., Geahlen RL., Post CB. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zhang Y., Oh H., Burton RA., Burgner JW., Geahlen RL., Post CB. DIP original experiment: DIP-133204X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Zhang Y., Oh H., Burton RA., Burgner JW., Geahlen RL., Post CB. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Zhang Y., Oh H., Burton RA., Burgner JW., Geahlen RL., Post CB. DIP original experiment: DIP-133205X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Tyr130 phosphorylation triggers Syk release from antigen receptor by long-distance conformational uncoupling. ksyk_mouse Tyrosine-protein kinase SYK Syk Spleen tyrosine kinase Sykb ptk72 protein protein mouse Mus musculus Mouse MAGSAVDSANHLTYFFGNITREEAEDYLVQGGMTDGLYLLRQSRNYLGGFALSVAHNRKAHHYTIERELNGTYAISGGRAHASPADLCHYHSQEPDGLICLLKKPFNRPPGVQPKTGPFEDLKENLIREYVKQTWNLQGQALEQAIISQKPQLEKLIATTAHEKMPWFHGNISRDESEQTVLIGSKTNGKFLIRARDNSGSYALCLLHEGKVLHYRIDRDKTGKLSIPEGKKFDTLWQLVEHYSYKPDGLLRVLTVPCQKIGAQMGHPGSPNAHPVTWSPGGIISRIKSYSFPKPGHKKPAPPQGSRPESTVSFNPYEPTGGPWGPDRGLQREALPMDTEVYESPYADPEEIRPKEVYLDRSLLTLEDNELGSGNFGTVKKGYYQMKKVVKTVAVKILKNEANDPALKDELLAEANVMQQLDNPYIVRMIGICEAESWMLVMEMAELGPLNKYLQQNRHIKDKNIIELVHQVSMGMKYLEESNFVHRDLAARNVLLVTQHYAKISDFGLSKALRADENYYKAQTHGKWPVKWYAPECINYYKFSSKSDVWSFGVLMWEAFSYGQKPYRGMKGSEVTAMLEKGERMGCPAGCPREMYDLMNLCWTYDVENRPGFTAVELRLRNYYYDVVN DIP protein P48025 original sequence version: 108 DIP protein P48025 original sequence version: 125 2F98D21601F8224E dpitam Doubly phosphorylated peptide ITAM peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ENLYEGLNLDDCSMYEDISR dpitam-syk-1 1 3 unspecified role unspecified role prey prey Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P48025:p.Tyr130Glu Y130E mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y E This feature has been corrected as a result of our quality control procedures. The original label was 'Y130E'. Wed May 02 18:24:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr130glu' Tue Jun 30 20:04:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr130Glu' chick Gallus gallus (Chicken) Chicken 4 unspecified role unspecified role bait bait in vitro In vitro physical association physical association dpitam-syk-2 2 3 unspecified role unspecified role unspecified role unspecified role Region 9-265 Region 9-265 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role in vitro In vitro direct interaction direct interaction