DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. Nature structural & molecular biology 2008 imex curation Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. DIP original experiment: DIP-113593X Nature structural & molecular biology 2008 IMEx A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. Nature structural & molecular biology 2008 imex curation Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. DIP original experiment: DIP-113594X Nature structural & molecular biology 2008 IMEx A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. Nature structural & molecular biology 2008 imex curation Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. DIP original experiment: DIP-113591X Nature structural & molecular biology 2008 IMEx A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. Nature structural & molecular biology 2008 imex curation Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Cordle J., Johnson S., Tay JZ., Roversi P., Wilkin MB., de Madrid BH., Shimizu H., Jensen S., Whiteman P., Jin B., Redfield C., Baron M., Lea SM., Handford PA. DIP original experiment: DIP-113592X Nature structural & molecular biology 2008 IMEx A conserved face of the Jagged/Serrate DSL domain is involved in Notch trans-activation and cis-inhibition. notc1_human Neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1 NOTCH1 TAN1 protein protein human Homo sapiens Human MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK DIP protein P46531 original sequence version: 173 DIP protein P46531 original sequence version: 103 DIP protein P46531 original sequence version: 109 DIP protein P46531 original sequence version: 127 DIP protein P46531 original sequence version: 137 DIP protein P46531 original sequence version: 186 DIP protein P46531 original sequence version: 150 DIP protein P46531 original sequence version: 142 DIP protein P46531 original sequence version: 181 E173C872D195F028 jag1_human Protein jagged-1 JAG1 JAGL1 protein protein human Homo sapiens Human MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV DIP protein P78504 original sequence version: 95 F36EE9FBF64DF162 jag1-notch1-2 3 6 unspecified role unspecified role bait bait Region 185-335 Region 185-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey Region 411-526 Region 411-526 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction jag1-notch1-3 1 6 unspecified role unspecified role bait bait Region 185-335 Region 185-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role prey prey Region 411-526 Region 411-526 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction notch1-jag1-1 4 6 unspecified role unspecified role prey prey Region 185-335 Region 185-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range P78504:p.Phe207Ala F207A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F A This feature has been corrected as a result of our quality control procedures. The original label was 'F207A'. Wed May 02 15:36:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'phe207ala' Tue Jun 30 19:39:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Phe207Ala' ecolx Escherichia coli 5 unspecified role unspecified role bait bait Region 411-526 Region 411-526 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Biotin-tag Biotin-tag biotin tag biotin tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction jag1-notch1-4 2 6 unspecified role unspecified role unspecified role unspecified role Region 185-335 Region 185-335 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 411-526 Region 411-526 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction