DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature 2008 imex curation Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. Only protein-protein interactions in vitro In vitro fps fluorescence polarization spectroscopy Fluorescence anisotropy FPS FPS Fluorescence anisotropy fps predetermined predetermined participant predetermined Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. DIP original experiment: DIP-250337X Nature 2008 IMEx The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature 2008 imex curation Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. DIP original experiment: DIP-250334X Nature 2008 IMEx The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature 2008 imex curation Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. Only protein-protein interactions in vitro In vitro solution sedimentati cosedimentation in solution solution sedimentati predetermined predetermined participant predetermined Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. DIP original experiment: DIP-250338X Nature 2008 IMEx The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature 2008 imex curation Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. DIP original experiment: DIP-250335X Nature 2008 IMEx The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. Nature 2008 imex curation Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Sallee NA., Rivera GM., Dueber JE., Vasilescu D., Mullins RD., Mayer BJ., Lim WA. DIP original experiment: DIP-250336X Nature 2008 IMEx The pathogen protein EspF(U) hijacks actin polymerization using mimicry and multivalency. wasl_rat Neural Wiskott-Aldrich syndrome protein Wasl protein protein rat Rattus norvegicus (Rat) Rat MSSGQQPPRRVTNVGSLLLTPQENESLFSFLGKKCVTMSSAVVQLYAADRNCMWSKKCSGVACLVKDNPQRSYFLRIFDIKDGKLLWEQELYNNFVYNSPRGYFHTFAGDTCQVALNFANEEEAKKFRKAVTDLLGRRQRKSEKRRDAPNGPNLPMATVDIKNPEITTNRFYSSQVNNISHTKEKKKGKAKKKRLTKADIGTPSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKNELRRQAPPPPPPSRGGPPPPPPPPHSSGPPPPPARGRGAPPPPPSRAPTAAPPPPPPSRPGVVVPPPPPNRMYPPPPPALPSSAPSGPPPPPPLSMAGSTAPPPPPPPPPPPGPPPPPGLPSDGDHQVPASSGNKAALLDQIREGAQLKKVEQNSRPVSCSGRDALLDQIRQGIQLKSVSDGQESTPPTPAPTSGIVGALMEVMQKRSKAIHSSDEDEDDDDEEDFQDDDEWED DIP protein O08816 original sequence version: 145 DIP protein O08816 original sequence version: 115 480E21F26F7FC77E esfu2_eco57 Secreted effector protein EspF(U) espF(U) tccP EspF-like protein encoded on prophage U Tir-cytoskeleton coupling protein TccP ECs2715 protein protein eco57 Escherichia coli O157:H7 MINNVSSLFPTVNRNITAVYKKSSFSVSPQKITLNPVKISSPFSPSSSSISATTLFRAPNAHSASFHRQSTAESSLHQQLPNVRQRLIQHLAEHGIKPARSMAEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLVQHLAEHGIQPARNMAEHIPPAPNWPAPPLPVQNEQSRPLPDVAQRLVQHLAEHGIQPARSMAEHIPPAPNWPAPPPPVQNEQSRPLPDVAQRLMQHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRPLPDVAQRLMQHLAEHGINTSKRS DIP protein P0DJ89 original sequence version: 5 This sequence has been withdrawn from Uniprot. 49A1BE2EA220E1C0 wasl-espfu-1 2 7 unspecified role unspecified role prey prey Region 221-267 Region 221-267 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait Region 228-270 Region 228-270 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction espfu-wasl-1 1 7 unspecified role unspecified role unspecified role unspecified role Region 80-126 Region 80-126 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Fluorescein-label Fluorescein-label fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled certain certain sequence position certain certain certain sequence position certain Region 226-274 Region 226-274 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction wasl-wasl-3 5 6 unspecified role unspecified role prey prey Region 190-274 Region 190-274 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 6 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 392-501 Region 392-501 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espfu-wasl-2 4 7 unspecified role unspecified role bait bait Region 221-237 Region 221-237 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 80-173 Region 80-173 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role prey prey His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Region 190-274 Region 190-274 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction espfu-wasl-3 3 7 unspecified role unspecified role unspecified role unspecified role Region 80-173 Region 80-173 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role Region 178-274 Region 178-274 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Region 392-501 Region 392-501 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction