DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Nature structural & molecular biology 2008 imex curation Zhou Z., Feng H., Hansen DF., Kato H., Luk E., Freedberg DI., Kay LE., Wu C., Bai Y. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Zhou Z., Feng H., Hansen DF., Kato H., Luk E., Freedberg DI., Kay LE., Wu C., Bai Y. DIP original experiment: DIP-113578X Nature structural & molecular biology 2008 IMEx NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. Nature structural & molecular biology 2008 imex curation Zhou Z., Feng H., Hansen DF., Kato H., Luk E., Freedberg DI., Kay LE., Wu C., Bai Y. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhou Z., Feng H., Hansen DF., Kato H., Luk E., Freedberg DI., Kay LE., Wu C., Bai Y. DIP original experiment: DIP-113579X Nature structural & molecular biology 2008 IMEx NMR structure of chaperone Chz1 complexed with histones H2A.Z-H2B. sh2b_h2a.z Chimera of Histone H2B.1 and Histone H2A.Z protein protein yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast RKETYSSYIYKVLKQTHPDTGISQKSMSILNSFVNDIFERIATEASKLAAYNKKSTISAREIQTAVRLILPGELAKHAVSEGTRAVTKYSSSTQAQSSSARAGLQFPVGRIKRYLKRHATGRTRVGSKAAIYLTAVLEYLTAEVLELAGNAAKDLKVKRITPRHLQLAIRGDDELDSLIRATIASGGVLPHI chz1_yeast Histone H2A.Z-specific chaperone CHZ1 CHZ1 YER030W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDEAKEKRELESQKESSHNKSEKSVEPKPKRRRRRNYDDYDAEVAKEETKAKNGLTKSENNGTVEDSESDMDDAKLDALMGNEGEEEEDDLAEIDTSNIITSGRRTRGKVIDYKKTAEELDKKEPSTGSKDDVGYGEKEEDDEDEEDDDFKE CE1DCBEFC035BC08 CE1DCBEFC035BC08 chz1-sh2b_h2a.z-1 1 3 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role Region 71-132 Region 71-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction chz1-sh2b_h2a.z-2 2 3 unspecified role unspecified role prey prey ecolx Escherichia coli 4 unspecified role unspecified role bait bait Region 71-132 Region 71-132 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ProteinA-tag ProteinA-tag protein a tag protein a tag protein A protein A n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro direct interaction direct interaction