DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature 2008 imex curation He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250329X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature 2008 imex curation He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250331X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. Nature 2008 imex curation He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined He X., Zhou J., Bartlam M., Zhang R., Ma J., Lou Z., Li X., Li J., Joachimiak A., Zeng Z., Ge R., Rao Z., Liu Y. DIP original experiment: DIP-250330X Nature 2008 IMEx Crystal structure of the polymerase PA(C)-PB1(N) complex from an avian influenza H5N1 virus. rdrp_i96a0 RNA-directed RNA polymerase catalytic subunit PB1 Polymerase basic protein 1 RNA-directed RNA polymerase subunit P1 protein protein i96a0 Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd) MDVNPTLLFLKVPAQNAISTTFPYTGDPPYSHGTGTGYTMDTVNRTHQYSEKGKWTTNTETGAPQLNPIDGPLPEDNEPSGYAQTDCVLEAMAFLEESHPGIFENSCLETMEVVQQTRVDKLTQGRQTYDWTLKRNQPAATALANTIEVFRSNGLTANESGRLIDFLKDVMESMDKGEMEIITHFQRKRRVRDNMTKKMVTQRTIGKKKQRLNKRSYLIRALTLNTMTKDAERGKLKRRAIATPGMQIRGFVYFVETLARSICEKLEQSGLPVGGNEKKAKLANVVRKMMTNSQDTELSFTITGDNTKWNENQNPRMFLAMITYITRNQPEWFRNVLSIAPIMFSNKMARLGKGYMFESKSMKLRTQIPAEMLASIDLKYFNESTRKKIEKIRPLLIDGTASLSPGMMMGMFNMLSTVLGVSILNLGQKRYTKTTYWWDGLQSSDDFALIVNAPNHEGIEAGVDRFYRTCKLVGINMTKKKSYINRTGTCEFTSFFYRYGFVANFSMELPSFGVSGINESADMSIGVTVIKNNMMDNDLGPATAQMALQLFIKDYRYPYRCHRGDTQIQTRRSFELKKLWEQTRSKAGLLVSDGGPNPYNIRNLHIPEAGLKWELMDEDYQGRLCNPLNPFVSHKEIESVNNAVVMPAHGPAKSMEYDAVATTHSWIPKRNRSILNTSQRGILEDEQMYQKCCNLFEKFFPSSSYRRPVGISSMVEAMVSRARIDARIDFESGRIKKEEFAEIMKICSTIEELGRQK DIP protein Q9Q0V0 original sequence version: 54 mint homomint D3F07429E0421159 pa_i96a0 Polymerase acidic protein PA RNA-directed RNA polymerase subunit P2 protein protein i96a0 Influenza A virus (strain A/Goose/Guangdong/1/1996 H5N1 genotype Gs/Gd) MEDFVRQCFNPMIVELAEKAMKEYGEDPKIETNKFAAICTHLEVCFMYSDFHFIDERGESTIIESGDPNALLKHRFEIIEGRDRTMAWTVVNSICNTTGVEKPKFLPDLYDYKENRFIEIGVTRREVHTYYLEKANKIKSEKTHIHIFSFTGEEMATKADYTLDEESRARIKTRLFTIRQEMASRGLWDSFRQSERGEETVEERFEITGTMCRLADQSLPPNFSSLEKFRAYVDGFEPNGCIEGKLSQMSKEVNARIEPFLKTTPRPLRLPDGPPCSQRSKFLLMDALKLSIEDPSHEGEGIPLYDAIKCMKTFFGWKEPNIVKPHEKGINPNYLLAWKQVLAELQDIENEEKIPKTKNMRKTSQLKWALGENMAPEKVDFEDCKDVSDLRQYDSDEPKPRSLASWIQSEFNKACELTDSSWIELDEIGEDVAPIEHIASMRRNYFTAEVSHCRATEYIMKGVYINTALLNASCAAMDDFQLIPMISKCRTKEGRRKTNLYGFLIKGRSHLRNDTDVVNFVSMEFSLTDPRLEPHRWEKYCVLRIGDMLLRTEIGQVSRPMFLYVRTNGTSKIKMKWGMEMRRCPFQSLQQIESMIEAESSVKEKDMTKEFFENKSETWPIGESPKGVEEGSIGKVCRTLLAKSVFNSLYASPQLEGFSAESRKLLLIVQALRDNLEPGTFDLGGLYEAIEECLINDPWVLLNASWFNSFLTHALR DIP protein Q9Q0U9 original sequence version: 51 pb1-pa-4 2 4 unspecified role unspecified role bait bait Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 5 unspecified role unspecified role prey prey Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9Q0U9:p.[Gln670Ala;Trp706Ala] W706/Q670A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A certain certain sequence position certain certain certain sequence position certain Q A Fri Jul 28 10:49:15 BST 2017 This feature has been corrected as a result of our quality control procedures. The original label was 'trp706ala,gln670ala' Wed May 02 15:16:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln670ala,trp706ala' Tue Jun 30 20:09:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Gln670Ala;Trp706Ala]' Q9Q0U9:p.[Leu666Gly;Phe710Glu] L666G/F710E/G mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F E certain certain sequence position certain certain certain sequence position certain L G Wed May 02 19:04:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu666gly,phe710glu' Tue Jun 30 20:20:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu666Gly;Phe710Glu]' Q9Q0U9:p.[Trp706Ala;Phe710Gln] W706/F710Q mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain F Q certain certain sequence position certain certain certain sequence position certain W A Wed May 02 14:57:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp706ala,phe710gln' Tue Jun 30 16:44:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Trp706Ala;Phe710Gln]' Q9Q0U9:p.[Leu666Gly;Phe710Gly] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain L G certain certain sequence position certain certain certain sequence position certain F G Wed May 02 16:42:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu666gly,phe710gly' Tue Jun 30 18:02:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Leu666Gly;Phe710Gly]' ecolx Escherichia coli direct interaction direct interaction pa-pb1-1 1 4 unspecified role unspecified role unspecified role unspecified role Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction pa-pb1-2 3 4 unspecified role unspecified role unspecified role unspecified role Region 1-25 Region 1-25 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role Region 257-716 Region 257-716 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction