DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure (London, England : 1993) 2008 imex curation Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. DIP original experiment: DIP-110801X Structure (London, England : 1993) 2008 IMEx The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure (London, England : 1993) 2008 imex curation Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. DIP original experiment: DIP-110805X Structure (London, England : 1993) 2008 IMEx The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure (London, England : 1993) 2008 imex curation Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. DIP original experiment: DIP-110803X Structure (London, England : 1993) 2008 IMEx The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure (London, England : 1993) 2008 imex curation Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. DIP original experiment: DIP-110804X Structure (London, England : 1993) 2008 IMEx The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. Structure (London, England : 1993) 2008 imex curation Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Schrader N., Koerner C., Koessmeier K., Bangert JA., Wittinghofer A., Stoll R., Vetter IR. DIP original experiment: DIP-110802X Structure (London, England : 1993) 2008 IMEx The crystal structure of the Ran-Nup153ZnF2 complex: a general Ran docking site at the nuclear pore complex. ran_human GTP-binding nuclear protein Ran GTPase Ran Ras-related nuclear protein Ras-like protein TC4 Androgen receptor-associated protein 24 RAN ARA24 OK/SW-cl.81 protein protein human Homo sapiens Human MAAQGEPQVQFKLVLVGDGGTGKTTFVKRHLTGEFEKKYVATLGVEVHPLVFHTNRGPIKFNVWDTAGQEKFGGLRDGYYIQAQCAIIMFDVTSRVTYKNVPNWHRDLVRVCENIPIVLCGNKVDIKDRKVKAKSIVFHRKKNLQYYDISAKSNYNFEKPFLWLARKLIGDPNLEFVAMPALAPPEVVMDPALAAQYEHDLEVAQTTALPDEDDDL DIP protein P62826 original sequence version: 138 DIP protein P62826 original sequence version: 67 DIP protein P62826 original sequence version: 93 DIP protein P62826 original sequence version: 125 DIP protein P62826 original sequence version: 96 D5C9B7275C34BCE0 nu153_rat Nuclear pore complex protein Nup153 Nup153 153 kDa nucleoporin Nucleoporin Nup153 protein protein rat Rattus norvegicus (Rat) Rat MASGAGGIGGGGGGGKIRTRRCHQGPVKPYQQGRPQHQGILSRVTESVKNIVPGWLQRYFNKSENACSCSVNADEVPRWPENREDEREIYVDENTNTDDGRTTPEPTGSNTEEPSTTSTASNYPDVLTRPSLHRSHLNFSVLESPALHCQPSTSSAFPIGSSGFSLVKEIKDSTSQHDDDNISTTSGFSSRASEKDIAVSKNTSLPPLWSPEAERSHSLSQHTAISSKKPAFNLSAFGTLSTSLGNSSILKTSQLGDSPFYPGKTTYGGAAAAVRQNKVRSTPYQAPVRRQMKAKQLNAQSYGVTSSTARRILQSLEKMSSPLADAKRIPSAVSSPLNSPLDRSGIDSTVFQAKKEKVDSQYPPVQRLMTPKPVSIATNRTVYFKPSLTPSGDLRKTNQRIDKKNSTVDEKNISRQNREQESGFSYPNFSIPAANGLSSGVGGGGGKMRRERTTHFVASKPSEEEEVEVPLLPQISLPISSSSLPTFNFSSPAISAASSSSVSPSQPLSNKVQMTSLGSTGNPVFTFSSPIVKSTQADVLPPASIGFTFSVPLAKTELSGPNSSSETVLSSSVTAQDNTVVNSSSSKKRSAPCEDPFTPAKILREGSVLDILKTPGFMSPKVDSPALQPTTTSSIVYTRPAISTFSSSGVEFGESLKAGSSWQCDTCLLQNKVTDNKCIACQAAKLPLKETAKQTGIGTPSKSDKPASTSGTGFGDKFKPAIGTWDCDTCLVQNKPEAVKCVACETPKPGTGVKRALPLTVASESPVTASSSTTVTTGTLGFGDKFKRPVGSWECPVCCVSNKAEDSRCVSCTSEKPGLVSASSSNSVPVSLPSGGCLGLDKFKKPEGSWDCEVCLVQNKADSTKCIACESAKPGTKSEFKGFGTSSSLNPAPSAFKFGIPSSSSGLSQTFTSTGNFKFGDQGGFKLGTSSDSGSTNTMNTNFKFPKPTGDFKFGVLPDSKPEEIKNDSKNDNFQFGPSSGLSNPASSAPFQFGVSTLGQQEKKEELPQSSSAGFSFGAGVANPSSAAIDTTVTSENKSGFNFGTIDTKSVSVTPFTYKTTEAKKEDASATKGGFTFGKVDSAALSSPSMFVLGRTEEKQQEPVTSTSLVFGKKADNEEPKCQPVFSFGNSEQTKDESSSKPTFSFSVAKPSVKESDQLAKATFAFGNQTNTTTDQGAAKPAFSFLNSSSSSSSTPATSSSASIFGSSTSSSSPPVAAFVFGQASNPVSSSAFGNSAESSTSQPLLFPQDGKPATTSSTASAAPPFVFGTGASSNSTVSSGFTFGATTTSSSSGSFFVFGTGHSAPSASPAFGANQTPTFGQSQGASQPNPPSFGSISSSTALFSAGSQPVPPPTFGTVSSSSQPPVFGQQPSQSAFGSGTANASSVFQFGSSTTNFNFTNNNPSGVFTFGASPSTPAAAAQPSGSGGFSFSQSPASFTVGSNGKNMFSSSGTSVSGRKIKTAVRRKK DIP protein P49791 original sequence version: 58 C3DFD9697C556A7C nup153-ran-1 1 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 703-754 Region 703-754 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nup153-ran-2 3 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 703-754 Region 703-754 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nup153-ran-3 4 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role Region 703-754 Region 703-754 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction nup153-ran-4 5 6 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait Region 703-754 Region 703-754 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain GSt-tag GSt-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction nup153-ran-5 2 6 unspecified role unspecified role unspecified role unspecified role yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 7 unspecified role unspecified role unspecified role unspecified role Region 703-754 Region 703-754 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast direct interaction direct interaction