DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Nature structural & molecular biology 2008 imex curation Lu X., Zhang Y., Shin YK. Only protein-protein interactions ecolx Escherichia coli epr electron paramagnetic resonance ESR EPR EPR ESR epr predetermined predetermined participant predetermined Lu X., Zhang Y., Shin YK. DIP original experiment: DIP-113612X Nature structural & molecular biology 2008 IMEx Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. Nature structural & molecular biology 2008 imex curation Lu X., Zhang Y., Shin YK. Only protein-protein interactions in vitro In vitro fret fluorescent resonance energy transfer FRET RET FRET analysis FRET RET FRET analysis fret predetermined predetermined participant predetermined Lu X., Zhang Y., Shin YK. DIP original experiment: DIP-113611X Nature structural & molecular biology 2008 IMEx Supramolecular SNARE assembly precedes hemifusion in SNARE-mediated membrane fusion. sso1_yeast Protein SSO1 SSO1 P1405 YPL232W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSYNNPYQLETPFEESYELDEGSSAIGAEGHDFVGFMNKISQINRDLDKYDHTINQVDSLHKRLLTEVNEEQASHLRHSLDNFVAQATDLQFKLKNEIKSAQRDGIHDTNKQAQAENSRQRFLKLIQDYRIVDSNYKEENKEQAKRQYMIIQPEATEDEVEAAISDVGGQQIFSQALLNANRRGEAKTALAEVQARHQELLKLEKSMAELTQLFNDMEELVIEQQENVDVIDKNVEDAQLDVEQGVGHTDKAVKSARKARKNKIRCWLIVFAIIVVVVVVVVVPAVVKTR 6E4C6D35C3CD9D94 6E4C6D35C3CD9D94 snc2_yeast Synaptobrevin homolog 2 SNC2 YOR327C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSVPYDPYVPPEESNSGANPNSQNKTAALRQEIDDTVGIMRDNINKVAERGERLTSIEDKADNLAISAQGFKRGANRVRKQMWWKDLKMRMCLFLVVIILLVVIIVPIVVHFS 815F027FAD8A599B 815F027FAD8A599B sso1p-snc2p-1 2 3 unspecified role unspecified role fluorescence donor fluorescence donor Region 185-290 Region 185-290 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Cy3-label Cy3-label cy3 label cy3 label certain certain sequence position certain certain certain sequence position certain P32867:p.Glu185Cys E185C mutation mutation certain certain sequence position certain certain certain sequence position certain E C This feature has been corrected as a result of our quality control procedures. The original label was 'E185C'. Wed May 02 16:16:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu185cys' Tue Jun 30 16:00:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu185Cys' ecolx Escherichia coli 4 unspecified role unspecified role fluorescence accept fluorescence acceptor fluorescence accept Cy5-label Cy5-label cy5 label cy5 label certain certain sequence position certain certain certain sequence position certain P33328:p.Pro13Cys P13C mutation mutation certain certain sequence position certain certain certain sequence position certain P C This feature has been corrected as a result of our quality control procedures. The original label was 'P13C'. Wed May 02 18:03:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro13cys' Tue Jun 30 15:55:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro13Cys' ecolx Escherichia coli direct interaction direct interaction sso1p-1 1 3 unspecified role unspecified role unspecified role unspecified role MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain MTSSL-label MTSSL-label r1 spin label r1 spin label certain certain sequence position certain certain certain sequence position certain Region 185-290 Region 185-290 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction