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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>DIP</shortLabel>
        <fullName>Database of Interacting Proteins</fullName>
      </names>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="identity" refTypeAc="MI:0356"/>
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      </xref>
      <attributeList>
        <attribute name="postaladdress">611 Young Drive East; Los Angeles CA 90095; USA</attribute>
        <attribute name="url" nameAc="MI:0614">http://dip.doe-mbi.ucla.edu/</attribute>
        <attribute name="contact-email" nameAc="MI:0634">dip@mbi.ucla.edu</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</fullName>
        </names>
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          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="18509058" refType="primary-reference" refTypeAc="MI:0358"/>
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            <attribute name="publication title" nameAc="MI:1091">Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</attribute>
            <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
            <attribute name="publication year" nameAc="MI:0886">2008</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Lee SY.,  Letts JA.,  Mackinnon R.</attribute>
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            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
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          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>crosslink</shortLabel>
            <fullName>cross-linking study</fullName>
            <alias type="synonym" typeAc="MI:1041">crosslink</alias>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0030" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Lee SY., Letts JA., Mackinnon R.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-111065X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2008</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</attribute>
        </attributeList>
      </experimentDescription>
      <experimentDescription id="2">
        <names>
          <fullName>Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</fullName>
        </names>
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            <attribute name="publication title" nameAc="MI:1091">Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</attribute>
            <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
            <attribute name="publication year" nameAc="MI:0886">2008</attribute>
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            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>bifunctional agent crosslink</shortLabel>
            <fullName>protein cross-linking with a bifunctional reagent</fullName>
            <alias type="go synonym" typeAc="MI:0303">Label transfer techniques</alias>
            <alias type="go synonym" typeAc="MI:0303">Photoaffinity labelling</alias>
            <alias type="synonym" typeAc="MI:1041">Label transfer techniques</alias>
            <alias type="synonym" typeAc="MI:1041">Photoaffinity labelling</alias>
            <alias type="synonym" typeAc="MI:1041">bifunctional agent crosslink</alias>
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            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0031" refType="identity" refTypeAc="MI:0356"/>
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        </interactionDetectionMethod>
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          <names>
            <shortLabel>western blot</shortLabel>
            <fullName>western blot</fullName>
            <alias type="go synonym" typeAc="MI:0303">Immuno blot</alias>
            <alias type="synonym" typeAc="MI:1041">Immuno blot</alias>
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          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0113" refType="identity" refTypeAc="MI:0356"/>
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        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Lee SY., Letts JA., Mackinnon R.</attribute>
          <attribute name="comment" nameAc="MI:0612">DIP original experiment: DIP-111066X</attribute>
          <attribute name="journal" nameAc="MI:0885">Proceedings of the National Academy of Sciences of the United States of America</attribute>
          <attribute name="publication year" nameAc="MI:0886">2008</attribute>
          <attribute name="curation depth" nameAc="MI:0955">IMEx</attribute>
          <attribute name="title" nameAc="MI:1091">Dimeric subunit stoichiometry of the human voltage-dependent proton channel Hv1.</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="3">
        <names>
          <shortLabel>hvcn1_human</shortLabel>
          <fullName>Voltage-gated hydrogen channel 1</fullName>
          <alias type="gene name" typeAc="MI:0301">HVCN1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">VSOP</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Hydrogen voltage-gated channel 1</alias>
          <alias type="gene name synonym" typeAc="MI:0302">Voltage sensor domain-only protein</alias>
          <alias type="orf name" typeAc="MI:0306">UNQ578/PRO1140</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="Q96D96" version="47" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000242607" version="SP_158" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000349181" version="SP_158" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSP00000479812" version="SP_158" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="A8MQ37" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="B4DEB3" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="F8WCH5" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q6UW11" refType="secondary-ac" refTypeAc="MI:0360"/>
          <secondaryRef db="uniprotkb" dbAc="MI:0486" id="Q96IS5" refType="secondary-ac" refTypeAc="MI:0360"/>
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          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000356742" version="SP_158" refType="transcript"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENST00000620084" version="SP_158" refType="transcript"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005886" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005887" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0009268" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0010043" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016021" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016324" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0022843" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030171" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030667" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030670" version="SP_158"/>
          <secondaryRef db="ensembl" dbAc="MI:0476" id="ENSG00000122986" version="SP_158" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0034765" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0035579" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0042802" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0045454" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0051453" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0071294" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0071467" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:1902600" version="SP_158"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005821" version="SP_158"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR027359" version="SP_158"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR031844" version="SP_158"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR031846" version="SP_158"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="3A2A" version="SP_158"/>
          <secondaryRef db="rcsb pdb" dbAc="MI:0460" id="5OQK" version="SP_158"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-1222556" version="SP_158"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-1300642" version="SP_158"/>
          <secondaryRef db="reactome" dbAc="MI:0467" id="R-HSA-6798695" version="SP_158"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0032930" version="SP_158"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001035196.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_001243342.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="NP_115745.2"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_005254005.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_011537147.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_011537148.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_011537149.1"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="XP_016875516.1"/>
          <secondaryRef db="dip" dbAc="MI:0465" id="DIP-46112N"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="9606">
          <names>
            <shortLabel>human</shortLabel>
            <fullName>Homo sapiens</fullName>
            <alias type="synonym" typeAc="MI:1041">Human</alias>
          </names>
        </organism>
        <sequence>MATWDEKAVTRRAKVAPAERMSKFLRHFTVVGDDYHAWNINYKKWENEEEEEEEEQPPPTPVSGEEGRAAAPDVAPAPGPAPRAPLDFRGMLRKLFSSHRFQVIIICLVVLDALLVLAELILDLKIIQPDKNNYAAMVFHYMSITILVFFMMEIIFKLFVFRLEFFHHKFEILDAVVVVVSFILDIVLLFQEHQFEALGLLILLRLWRVARIINGIIISVKTRSERQLLRLKQMNVQLAAKIQHLEFSCSEKEQEIERLNKLLRQHGLLGEVN</sequence>
        <attributeList>
          <attribute name="remark-internal">DIP protein Q96D96 original sequence version: 47</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="4" imexId="IM-9360-1">
        <names>
          <shortLabel>hvcn1-1</shortLabel>
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          <primaryRef db="dip" dbAc="MI:0465" id="DIP-87901E" refType="identity" refTypeAc="MI:0356"/>
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        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="5">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
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                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
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                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
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          </xref>
        </interactionType>
      </interaction>
      <interaction id="6" imexId="IM-9360-2">
        <names>
          <shortLabel>hvcn1-2</shortLabel>
        </names>
        <xref>
          <primaryRef db="dip" dbAc="MI:0465" id="DIP-87901E" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-15705029" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="psi-mi" dbAc="MI:0488" id="MI:0465" refType="imex source"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-9360-2" refType="imex-primary" refTypeAc="MI:0662"/>
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        <experimentList>
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        </experimentList>
        <participantList>
          <participant id="7">
            <interactorRef>3</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
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            </biologicalRole>
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                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
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              </experimentalRole>
            </experimentalRoleList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="9606">
                <names>
                  <shortLabel>human</shortLabel>
                  <fullName>Homo sapiens</fullName>
                  <alias type="synonym" typeAc="MI:1041">Human</alias>
                </names>
              </hostOrganism>
            </hostOrganismList>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
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        </interactionType>
      </interaction>
    </interactionList>
  </entry>
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