DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259405X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro fluorescence spectr classical fluorescence spectroscopy fluorescence spectr predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259411X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259412X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259415X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259413X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259408X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259414X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259417X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259407X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259404X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259406X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259416X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259410X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Proteasome subunit Rpn13 is a novel ubiquitin receptor. Nature 2008 imex curation Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Husnjak K., Elsasser S., Zhang N., Chen X., Randles L., Shi Y., Hofmann K., Walters KJ., Finley D., Dikic I. DIP original experiment: DIP-259409X Nature 2008 IMEx Proteasome subunit Rpn13 is a novel ubiquitin receptor. adrm1_human Proteasomal ubiquitin receptor ADRM1 Adhesion-regulating molecule 1 110 kDa cell membrane glycoprotein Rpn13 homolog Proteasome regulatory particle non-ATPase 13 ADRM1 GP110 protein protein human Homo sapiens Human MTTSGALFPSLVPGSRGASNKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGNVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNEYLNNPPMPGALGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPGSSSSSSSRSQSAAVTPSSTTSSTRATPAPSAPAAASATSPSPAPSSGNGASTAASPTQPIQLSDLQSILATMNVPAGPAGGQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKPEQKEGDTKDKKDEEEDMSLD DIP protein Q16186 original sequence version: 116 DIP protein Q16186 original sequence version: 93 2D38811DCA231864 rpn13_yeast 26S proteasome regulatory subunit RPN13 RPN13 DAQ1 Proteasome non-ATPase subunit 13 YLR421C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSMSSTVIKFRAGVCEYNEDSRLCTPIPVQGEIEIKPNEEEELGFWDFEWRPTEKPVGRELDPISLILIPGETMWVPIKSSKSGRIFALVFSSNERYFFWLQEKNSGNLPLNELSAKDKEIYNKMIGVLNNSSESDEEESNDEKQKAQDVDVSMQD 752E8A12DC3799D4 752E8A12DC3799D4 p54727-1 UV excision repair protein RAD23 homolog B RAD23B XP-C repair-complementing complex 58 kDa protein protein protein human Homo sapiens Human MQVTLKTLQQQTFKIDIDPEETVKALKEKIESEKGKDAFPVAGQKLIYAGKILNDDTALKEYKIDEKNFVVVMVTKPKAVSTPAPATTQQSAPASTTAVTSSTTTTVAQAPTPVPALAPTSTPASITPASATASSEPAPASAAKQEKPAEKPAETPVATSPTATDSTSGDSSRSNLFEDATSALVTGQSYENMVTEIMSMGYEREQVIAALRASFNNPDRAVEYLLMGIPGDRESQAVVDPPQAASTGAPQSSAVAAAAATTTATTTTTSSGGHPLEFLRNQPQFQQMRQIIQQNPSLLPALLQQIGRENPQLLQQISQHQEHFIQMLNEPVQEAGGQGGGGGGGSGGIAEAGSGHMNYIQVTPQEKEAIERLKALGFPEGLVIQAYFACEKNENLAANFLLQQNFDED DIP protein P54727-1 original sequence version: 154 DIP protein P54727 original sequence version: 139 ddi1_human Protein DDI1 homolog 1 DDI1 protein protein human Homo sapiens Human MLITVYCVRRDLSEVTFSLQVSPDFELRNFKVLCEAESRVPVEEIQIIHMERLLIEDHCSLGSYGLKDGDIVVLLQKDNVGPRAPGRAPNQPRVDFSGIAVPGTSSSRPQHPGQQQQRTPAAQRSQGLASGEKVAGLQGLGSPALIRSMLLSNPHDLSLLKERNPPLAEALLSGSLETFSQVLMEQQREKALREQERLRLYTADPLDREAQAKIEEEIRQQNIEENMNIAIEEAPESFGQVTMLYINCKVNGHPLKAFVDSGAQMTIMSQACAERCNIMRLVDRRWAGVAKGVGTQRIIGRVHLAQIQIEGDFLQCSFSILEDQPMDMLLGLDMLRRHQCSIDLKKNVLVIGTTGTQTYFLPEGELPLCSRMVSGQDESSDKEITHSVMDSGRKEH DIP protein Q8WTU0 original sequence version: 94 1127CBF7ED3C705D ubql2_human Ubiquilin-2 UBQLN2 PLIC2 HRIHFB2157 N4BP4 Protein linking IAP with cytoskeleton 2 Ubiquitin-like product Chap1/Dsk2 DSK2 homolog Chap1 protein protein human Homo sapiens Human MAENGESSGPPRPSRGPAAAQGSAAAPAEPKIIKVTVKTPKEKEEFAVPENSSVQQFKEAISKRFKSQTDQLVLIFAGKILKDQDTLIQHGIHDGLTVHLVIKSQNRPQGQSTQPSNAAGTNTTSASTPRSNSTPISTNSNPFGLGSLGGLAGLSSLGLSSTNFSELQSQMQQQLMASPEMMIQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHLLNNPDIMRQTLEIARNPAMMQEMMRNQDLALSNLESIPGGYNALRRMYTDIQEPMLNAAQEQFGGNPFASVGSSSSSGEGTQPSRTENRDPLPNPWAPPPATQSSATTSTTTSTGSGSGNSSSNATGNTVAAANYVASIFSTPGMQSLLQQITENPQLIQNMLSAPYMRSMMQSLSQNPDLAAQMMLNSPLFTANPQLQEQMRPQLPAFLQQMQNPDTLSAMSNPRAMQALMQIQQGLQTLATEAPGLIPSFTPGVGVGVLGTAIGPVGPVTPIGPIGPIVPFTPIGPIGPIGPTGPAAPPGSTGSGGPTGPTVSSAAPSETTSPTSESGPNQQFIQQMVQALAGANAPQLPNPEVRFQQQLEQLNAMGFLNREANLQALIATGGDINAAIERLLGSQPS DIP protein Q9UHD9 original sequence version: 108 DIP protein Q9UHD9 original sequence version: 113 DF7DF8C4D7B71AC3 adrm1_mouse Proteasomal ubiquitin receptor ADRM1 Adrm1 Gp110 110 kDa cell membrane glycoprotein Adhesion-regulating molecule 1 Rpn13 homolog protein protein mouse Mus musculus Mouse MTTSGALFPSLVPGSRGSSTKYLVEFRAGKMSLKGTTVTPDKRKGLVYIQQTDDSLIHFCWKDRTSGTVEDDLIIFPDDCEFKRVPQCPSGRVYVLKFKAGSKRLFFWMQEPKTDQDEEHCRKVNECLNNPPMPGSLGASGSSGHELSALGGEGGLQSLLGNMSHSQLMQLIGPAGLGGLGGLGALTGPGLASLLGSSGPPASSSSSSSRSQSAAVTPSSSTSSARATPAPSAPAAASATSPSPAPSSGNGTSTAASPTQPIQLSDLQSILATMNVPAGPGGSQQVDLASVLTPEIMAPILANADVQERLLPYLPSGESLPQTADEIQNTLTSPQFQQALGMFSAALASGQLGPLMCQFGLPAEAVEAANKGDVEAFAKAMQNNAKSDPKEGDTKDKKDEEEDMSLD DIP protein Q9JKV1 original sequence version: 86 210AFDF7379C962D rd23a_human UV excision repair protein RAD23 homolog A RAD23A protein protein human Homo sapiens Human MAVTITLKTLQQQTFKIRMEPDETVKVLKEKIEAEKGRDAFPVAGQKLIYAGKILSDDVPIRDYRIDEKNFVVVMVTKTKAGQGTSAPPEASPTAAPESSTSFPPAPTSGMSHPPPAAREDKSPSEESAPTTSPESVSGSVPSSGSSGREEDAASTLVTGSEYETMLTEIMSMGYERERVVAALRASYNNPHRAVEYLLTGIPGSPEPEHGSVQESQVSEQPATEAAGENPLEFLRDQPQFQNMRQVIQQNPALLPALLQQLGQENPQLLQQISRHQEQFIQMLNEPPGELADISDVEGEVGAIGEEAPQMNYIQVTPQEKEAIERLKALGFPESLVIQAYFACEKNENLAANFLLSQNFDDE DIP protein P54725 original sequence version: 141 DIP protein P54725 original sequence version: 136 DIP protein P54725 original sequence version: 139 C4E47E9313BB47B5 ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG q99460-1 26S proteasome non-ATPase regulatory subunit 1 PSMD1 26S proteasome regulatory subunit RPN2 26S proteasome regulatory subunit S1 26S proteasome subunit p112 protein protein human Homo sapiens Human MITSAAGIISLLDEDEPQLKEFALHKLNAVVNDFWAEISESVDKIEVLYEDEGFRSRQFAALVASKVFYHLGAFEESLNYALGAGDLFNVNDNSEYVETIIAKCIDHYTKQCVENADLPEGEKKPIDQRLEGIVNKMFQRCLDDHKYKQAIGIALETRRLDVFEKTILESNDVPGMLAYSLKLCMSLMQNKQFRNKVLRVLVKIYMNLEKPDFINVCQCLIFLDDPQAVSDILEKLVKEDNLLMAYQICFDLYESASQQFLSSVIQNLRTVGTPIASVPGSTNTGTVPGSEKDSDSMETEEKTSSAFVGKTPEASPEPKDQTLKMIKILSGEMAIELHLQFLIRNNNTDLMILKNTKDAVRNSVCHTATVIANSFMHCGTTSDQFLRDNLEWLARATNWAKFTATASLGVIHKGHEKEALQLMATYLPKDTSPGSAYQEGGGLYALGLIHANHGGDIIDYLLNQLKNASNDIVRHGGSLGLGLAAMGTARQDVYDLLKTNLYQDDAVTGEAAGLALGLVMLGSKNAQAIEDMVGYAQETQHEKILRGLAVGIALVMYGRMEEADALIESLCRDKDPILRRSGMYTVAMAYCGSGNNKAIRRLLHVAVSDVNDDVRRAAVESLGFILFRTPEQCPSVVSLLSESYNPHVRYGAAMALGICCAGTGNKEAINLLEPMTNDPVNYVRQGALIASALIMIQQTEITCPKVNQFRQLYSKVINDKHDDVMAKFGAILAQGILDAGGHNVTISLQSRTGHTHMPSVVGVLVFTQFWFWFPLSHFLSLAYTPTCVIGLNKDLKMPKVQYKSNCKPSTFAYPAPLEVPKEKEKEKVSTAVLSITAKAKKKEKEKEKKEEEKMEVDEAEKKEEKEKKKEPEPNFQLLDNPARVMPAQLKVLTMPETCRYQPFKPLSIGGIIILKDTSEDIEELVEPVAAHGPKIEEEEQEPEPPEPFEYIDD DIP protein Q99460-1 original sequence version: 128 ub-adrm1-1 10 15 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 22 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association ddi1-adrm1-1 8 18 unspecified role unspecified role bait bait Region 1-81 Region 1-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 20 unspecified role unspecified role prey prey Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction scrpn13-ub-1 14 16 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 22 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction adrm1-rad23a-1 5 21 unspecified role unspecified role unspecified role unspecified role Region 1-81 Region 1-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction adrm1-ub-1 1 20 unspecified role unspecified role bait bait Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 22 unspecified role unspecified role prey prey ile44ala I44A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I44A'. yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association adrm1-ub-2 13 15 unspecified role unspecified role unspecified role unspecified role Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 22 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction adrm1-ubqln2-1 7 19 unspecified role unspecified role unspecified role unspecified role Region 33-107 Region 33-107 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 15 unspecified role unspecified role unspecified role unspecified role Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction adrm1-psmd1-1 11 20 unspecified role unspecified role bait bait yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 23 unspecified role unspecified role prey prey yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast physical association physical association adrm1-ub-3 2 15 unspecified role unspecified role unspecified role unspecified role Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 22 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction adrm1-ub-4 9 20 unspecified role unspecified role bait bait Region 1-110 Region 1-110 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 22 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction rad23a-adrm1-1 4 21 unspecified role unspecified role bait bait Region 1-81 Region 1-81 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 20 unspecified role unspecified role prey prey Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ub-adrm1-2 3 20 unspecified role unspecified role prey prey Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 22 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal ile44ala I44A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'I44A'. leu8ala,ile44ala,val70ala L8A/I44A/V70A mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength alanine scanning alanine scanning certain certain sequence position certain certain certain sequence position certain L A certain certain sequence position certain certain certain sequence position certain V A certain certain sequence position certain certain certain sequence position certain I A This feature has been corrected as a result of our quality control procedures. The original label was 'L8A/I44A/V70A'. ecolx Escherichia coli direct interaction direct interaction scrpn13-ub-2 6 16 unspecified role unspecified role bait bait GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 22 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction rad23b-adrm1-2 12 17 unspecified role unspecified role bait bait Region 1-79 Region 1-79 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain GST-tag GST-tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli 20 unspecified role unspecified role prey prey Region 1-150 Region 1-150 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction