DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113635X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113637X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113634X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113636X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113633X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). Nature structural & molecular biology 2008 imex curation Zhang CM., Liu C., Slater S., Hou YM. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Zhang CM., Liu C., Slater S., Hou YM. DIP original experiment: DIP-113638X Nature structural & molecular biology 2008 IMEx Aminoacylation of tRNA with phosphoserine for synthesis of cysteinyl-tRNA(Cys). seps_metja O-phosphoserine--tRNA(Cys) ligase sepS Non-canonical O-phosphoseryl-tRNA(Cys) synthetase O-phosphoseryl-tRNA(Cys) synthetase MJ1660 protein protein metja Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) Methanococcus jannaschii MRFDIKKVLELAEKDFETAWRETRALIKDKHIDNKYPRLKPVYGKPHPVMETIERLRQAYLRMGFEEMINPVIVDEMEIYKQFGPEAMAVLDRCFYLAGLPRPDVGLGNEKVEIIKNLGIDIDEEKKERLREVLHLYKKGAIDGDDLVFEIAKALNVSNEMGLKVLETAFPEFKDLKPESTTLTLRSHMTSGWFITLSSLIKKRKLPLKLFSIDRCFRREQREDRSHLMSYHSASCVVVGEDVSVDDGKVVAEGLLAQFGFTKFKFKPDEKKSKYYTPETQTEVYAYHPKLGEWIEVATFGVYSPIALAKYNIDVPVMNLGLGVERLAMIIYGYEDVRAMVYPQFYEYRLSDRDIAGMIRVDKVPILDEFYNFANELIDICIANKDKESPCSVEVKREFNFNGERRVIKVEIFENEPNKKLLGPSVLNEVYVYDGNIYGIPPTFEGVKEQYIPILKKAKEEGVSTNIRYIDGIIYKLVAKIEEALVSNVDEFKFRVPIVRSLSDINLKIDELALKQIMGENKVIDVRGPVFLNAKVEIK 301201548E9ADE40 spss_metja O-phospho-L-seryl-tRNA:Cys-tRNA synthase pscS Sep-tRNA:Cys-tRNA synthase MJ1678 protein protein metja Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440) Methanococcus jannaschii MDKYKNLTRSLTREFINLNPIQRGGILPKEAKKAVYEYWDGYSVCDYCHGRLDEVTCPPIKDFLEDIAKFLNMDCARPTHGAREGKFIVMHAICKEGDYVVLDKNAHYTSYVAAERAKLNVAEVGYEEEYPTYKINLEGYKEVIDNLEDKGKNVGLILLTHVDGEYGNLNDAKKVGKIAKEKGIPFLLNCAYTVGRMPVNGKEVKADFIVASGHKSMAASAPCGILAFSEEFSDKITKTSEKFPVKEIEMLGCTSRGLPIVTLMASFPHVVERVKKWDEELKKTRYVVDELEKIGFKQLGIKPKEHDLIKFETPVLDEIAKKDKRRGFFFYDELKKRGIGGIRAGVTKEIKMSVYGLEWEQVEYVVNAIKEIVESCK 791842FF733C72BA seprs-5 5 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction sepcyss-seprs-1 2 8 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction sepcyss-seprs-2 3 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 7 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction sepcyss-1 1 8 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction seprs-sepcyss-1 6 8 unspecified role unspecified role prey prey ecolx Escherichia coli 7 unspecified role unspecified role bait bait His-tag His-tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecolx Escherichia coli direct interaction direct interaction sepcyss-seprs-3 4 8 unspecified role unspecified role bait bait ecolx Escherichia coli 7 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction