DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68373X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68374X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68375X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68376X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68372X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68370X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. Proceedings of the National Academy of Sciences of the United States of America 2008 imex curation Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Kittler JT., Chen G., Kukhtina V., Vahedi-Faridi A., Gu Z., Tretter V., Smith KR., McAinsh K., Arancibia-Carcamo IL., Saenger W., Haucke V., Yan Z., Moss SJ. DIP original experiment: DIP-68371X Proceedings of the National Academy of Sciences of the United States of America 2008 IMEx Regulation of synaptic inhibition by phospho-dependent binding of the AP2 complex to a YECL motif in the GABAA receptor gamma2 subunit. d3zuy8_rat AP-2 complex subunit alpha Ap2a1 Ap2a1_predicted rCG_54084 protein protein rat Rattus norvegicus (Rat) Rat MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTHLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF 5704048BEC825FDF 5704048BEC825FDF yeclphos GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) ATHLQERDEEYGYECLDGKDC ap2m1_rat AP-2 complex subunit mu Adaptor protein complex AP-2 subunit mu AP-2 mu chain Ap2m1 Mu2-adaptin Plasma membrane adaptor AP-2 50 kDa protein Clathrin coat assembly protein AP50 Clathrin coat-associated protein AP50 Clathrin assembly protein complex 2 mu medium chain Adaptor-related protein complex 2 subunit mu protein protein rat Rattus norvegicus (Rat) Rat MIGGLFIYNHKGEVLISRVYRDDIGRNAVDAFRVNVIHARQQVRSPVTNIARTSFFHVKRSNIWLAAVTKQNVNAAMVFEFLYKMCDVMAAYFGKISEENIKNNFVLIYELLDEILDFGYPQNSETGALKTFITQQGIKSQHQTKEEQSQITSQVTGQIGWRREGIKYRRNELFLDVLESVNLLMSPQGQVLSAHVSGRVVMKSYLSGMPECKFGMNDKIVIEKQGKGTADETSKSGKQSIAIDDCTFHQCVRLSKFDSERSISFIPPDGEFELMRYRTTKDIILPFRVIPLVREVGRTKLEVKVVIKSNFKPSLLAQKIEVRIPTPLNTSGVQVICMKGKAKYKASENAIVWKIKRMAGMKESQISAEIELLPTNDKKKWARPPISMNFEVPFAPSGLKVRYLKVFEPKLNYSDHDVIKWVRYIGRSGIYETRC DIP protein P84092 original sequence version: 91 DIP protein P84092 original sequence version: 42 DIP protein P84092 original sequence version: 82 DIP protein P84092 original sequence version: 67 DIP protein P84092 original sequence version: 92 DIP protein P84092 original sequence version: 105 DIP protein P84092 original sequence version: 110 82803219BA279954 ap2a2_rat AP-2 complex subunit alpha-2 Adaptor protein complex AP-2 subunit alpha-2 Alpha2-adaptin Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit Alpha-adaptin C Clathrin assembly protein complex 2 alpha-C large chain 100 kDa coated vesicle protein C Ap2a2 Adtab Adaptor-related protein complex 2 subunit alpha-2 protein protein rat Rattus norvegicus (Rat) Rat MPAVSKGEGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNRYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMGLALHCIANVGSREMAEAFAGEIPKILVAGDTMDSVKQSAALCLLRLYRTSPDLVPMGDWTSRVVHLLNDQHLGVVTAATSLITTLAQKNPEEFKTSVSLAVSRLSRIVTSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPDPAVRGRLTECLETILNKAQEPPKSKKVQHSNAKNAVLFEAISLIIHHDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIETVINALKTERDVSVRQRAVDLLYAMCDRSNAQQIVAEMLSYLETADYSIREEIVLKVAILAEKYAVDYTWYVDTILNLIRIAGDYVSEEVWYRVIQIVINRDDVQGYAAKTVFEALQAPACHENLVKVGGYILGEFGNLIAGDPRSSPLIQFNLLHSKFHLCSVPTRALLLSTYIKFVNLFPEVKATIQDVLRSDSQLKNADVELQQRAVEYLRLSTVASTDILATVLEEMPPFPERESSILAKLKKKKGPSTVTDLEETKRERSIDVNGGPEPVPASTSAASTPSPSADLLGLGAVPPAPTGPPPSSGGGLLVDVFSDSASAVAPLAPGSEDNFARFVCKNNGVLFENQLLQIGLKSEFRQNLGRMFIFYGNKTSTQFLNFTPTLICADDLQTNLNLQTKPVDPTVDGGAQVQQVINIECISDFTEAPVLNIQFRYGGTFQNVSVKLPITLNKFFQPTEMASQDFFQRWKQLSNPQQEVQNIFKAKHPMDTEITKAKIIGFGSALLEEVDPNPANFVGAGIIHTKTTQIGCLLRLEPNLQAQMYRLTLRTSKDTVSQRLCELLSEQF DIP protein P18484 original sequence version: 80 DIP protein P18484 original sequence version: 124 DIP protein P18484 original sequence version: 134 DIP protein P18484 original sequence version: 140 406428A9BFFB1E53 yeclpep GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) EYGYECLDG ygyecl GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YGYECL deeygyecld GABA(A) receptor subunit gamma-2 peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) DEEYGYECLD yeclphos-ap2m1-1 3 9 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey in vitro In vitro direct interaction direct interaction ap2m1-deeygyecld-1 6 14 unspecified role unspecified role unspecified role unspecified role in vitro In vitro 10 unspecified role unspecified role unspecified role unspecified role Region 158-435 Region 158-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction yeclpep-ap2m1-1 1 12 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey Region 156-435 Region 156-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction ygyecl-ap2m1-1 7 13 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey ecolx Escherichia coli direct interaction direct interaction yeclphos-ap2 alpha1-1 4 8 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat 11 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat 9 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat association association yeclphos-ap2m1-2 2 9 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey rat Rattus norvegicus (Rat) Rat physical association physical association yeclpep-ap2m1-2 5 12 unspecified role unspecified role bait bait in vitro In vitro 10 unspecified role unspecified role prey prey Region 158-435 Region 158-435 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction