DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Nature structural & molecular biology 2008 imex curation Hou JY., Sauer RT., Baker TA. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hou JY., Sauer RT., Baker TA. DIP original experiment: DIP-132542X Nature structural & molecular biology 2008 IMEx Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Nature structural & molecular biology 2008 imex curation Hou JY., Sauer RT., Baker TA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hou JY., Sauer RT., Baker TA. DIP original experiment: DIP-132541X Nature structural & molecular biology 2008 IMEx Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. Nature structural & molecular biology 2008 imex curation Hou JY., Sauer RT., Baker TA. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hou JY., Sauer RT., Baker TA. DIP original experiment: DIP-132543X Nature structural & molecular biology 2008 IMEx Distinct structural elements of the adaptor ClpS are required for regulating degradation by ClpAP. clps_ecoli ATP-dependent Clp protease adapter protein ClpS yljA clpS b0881 JW0865 protein protein ecoli Escherichia coli (strain K12) MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVIDVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCTLEKA DIP protein P0A8Q6 original sequence version: 43 2E9DC8000EA3CE86 peptide-2 Signal peptide peptide peptide polypeptide oligopeptide oligopeptide polypeptide chemical synthesis Chemical synthesis (Chemical synthesis) YLFVQ clpa_ecoli ATP-dependent Clp protease ATP-binding subunit ClpA clpA lopD b0882 JW0866 protein protein ecoli Escherichia coli (strain K12) MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREALEACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNEVTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSEEQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGVGKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQDTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKDRALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDRHLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLKNLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQLSKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEIEKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDNSTDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEVSQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKNELTYGFQSAQKHKAEAAH DIP protein P0ABH9 original sequence version: 77 DIP protein P0ABH9 original sequence version: 40 DIP protein P0ABH9 original sequence version: 38 0C96A2EB64A9F7DC clpa-clps-7 2 4 unspecified role unspecified role unspecified role unspecified role Region 18-106 Region 18-106 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli 6 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction clpa-clps-8 1 4 unspecified role unspecified role prey prey ecolx Escherichia coli 6 unspecified role unspecified role bait bait ecolx Escherichia coli direct interaction direct interaction clps-peptide-1 3 5 unspecified role unspecified role unspecified role unspecified role GFP-tag GFP-tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal c-terminal c-terminal position c-term carboxy-terminus c-terminus c-term c-terminus carboxy-terminus c-terminal in vitro In vitro 4 unspecified role unspecified role unspecified role unspecified role Region 18-106 Region 18-106 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction