DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature 2008 imex curation Roll-Mecak A., Vale RD. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly light scattering light scattering predetermined predetermined participant predetermined Roll-Mecak A., Vale RD. DIP original experiment: DIP-250319X Nature 2008 IMEx Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature 2008 imex curation Roll-Mecak A., Vale RD. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly saxs x ray scattering saxs predetermined predetermined participant predetermined Roll-Mecak A., Vale RD. DIP original experiment: DIP-250318X Nature 2008 IMEx Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature 2008 imex curation Roll-Mecak A., Vale RD. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Roll-Mecak A., Vale RD. DIP original experiment: DIP-250317X Nature 2008 IMEx Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. Nature 2008 imex curation Roll-Mecak A., Vale RD. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly light scattering light scattering predetermined predetermined participant predetermined Roll-Mecak A., Vale RD. DIP original experiment: DIP-250320X Nature 2008 IMEx Structural basis of microtubule severing by the hereditary spastic paraplegia protein spastin. spast_drome Spastin spas D-Spastin Dm-Spastin Dspastin CG5977 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVRTKNQSSSSSASSSSTKSPIKSSSGAGSSGGGLGGRQSTHRSSSASNVAAVVAGGSSAAGGGSSSNRRSPGSSPDGDDDTTTTDDLTPTTCSPRSGHHHSYGGYSSSVHKQNLYVVSFPIIFLFNVLRSLIYQLFCIFRYLYGASTKVIYRPHRRDCNIEIVVQNSSKEQQQSLNHPSELNREGDGQEQQLSNQPQRFRPIQPLEMAANRPGGGYSPGPGDPLLAKQKHHHRRAFEYISKALKIDEENEGHKELAIELYRKGIKELEDGIAVDCWSGRGDVWDRAQRLHDKMQTNLSMARDRLHFLALREQDLQMQRLSLKEKQKEEAQSKPQKTREPMLAGMTNEPMKLRVRSSGYGPKATTSAQPTASGRKLTIGSKRPVNLAVANKSQTLPRNLGSKTSVGAVQRQPAKTAATPPAVRRQFSSGRNTPPQRSRTPINNNGPSGSGASTPVVSVKGVEQKLVQLILDEIVEGGAKVEWTDIAGQDVAKQALQEMVILPSVRPELFTGLRAPAKGLLLFGPPGNGKTLLARAVATECSATFLNISAASLTSKYVGDGEKLVRALFAVARHMQPSIIFIDEVDSLLSERSSSEHEASRRLKTEFLVEFDGLPGNPDGDRIVVLAATNRPQELDEAALRRFTKRVYVSLPDEQTRELLLNRLLQKQGSPLDTEALRRLAKITDGYSGSDLTALAKDAALEPIRELNVEQVKCLDISAMRAITEQDFHSSLKRIRRSVAPQSLNSYEKWSQDYGDITI This sequence has been withdrawn from Uniprot. 406D075DCBD2A9CD 406D075DCBD2A9CD spast_caeel Spastin homolog spas-1 C24B5.2 protein protein caeel Caenorhabditis elegans MFAFSKGPAGSSTYDRVAQKFQDGYEKMRAAIEMDELTKHAGSIQEKLRTAELYKEARSLLKEANEFNIMDIPETRRSEIRDKRQNMMKLEKSAQDRLIAICNEVDPNVKQSRSATVGPSRPASAARVTPRPTRATAPEKKNAAKAKENDENRHVCSRGDRCGAHHQPVTKKSDTVHPEPPVQASNRKMETVKRVKVDKASLPMHQNPVNRAALLNGVDKVIGERLLDEVLDNTGVRMDDVAGCHSAKAALEEAVILPALNPNLFKGLRQPVKGILLFGPPGNGKTLLAKAVAGESKQMFFNISASSLTSKWVGDSEKTIRGLFQIARNAQPSIIFIDEIDSILCERSEKDAEVSRRMKTEFLVQFDGATSSADDRILVIGATNRPHELDDAVLRRFPKRIMLNLPDEEARKELITKTLKKHNMMDGLISSDIRYIASNTSGFSNSDLVALCKEAAMVPIREIDRSKLSMTDGEKIRKIRASDFDTALRTIRPSTSQKIMSKLSDFSRSFGC DIP protein Q8MNV0 original sequence version: 79 aaa-1 4 5 unspecified role unspecified role unspecified role unspecified role Region 208-696 Region 208-696 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction spas_1-1 3 6 unspecified role unspecified role unspecified role unspecified role Region 15-451 Region 15-451 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction spas_1-2 2 6 unspecified role unspecified role unspecified role unspecified role Region 15-451 Region 15-451 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction spas_1-3 1 6 unspecified role unspecified role unspecified role unspecified role Region 15-451 Region 15-451 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain drome Drosophila melanogaster (Fruit fly) Fruit fly direct interaction direct interaction