DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Nature 2008 imex curation Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. DIP original experiment: DIP-250289X Nature 2008 IMEx Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Nature 2008 imex curation Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. DIP original experiment: DIP-250292X Nature 2008 IMEx Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Nature 2008 imex curation Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. Only protein-protein interactions drome Drosophila melanogaster (Fruit fly) Fruit fly anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. DIP original experiment: DIP-250290X Nature 2008 IMEx Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. Nature 2008 imex curation Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Hanyu-Nakamura K., Sonobe-Nojima H., Tanigawa A., Lasko P., Nakamura A. DIP original experiment: DIP-250291X Nature 2008 IMEx Drosophila Pgc protein inhibits P-TEFb recruitment to chromatin in primordial germ cells. o17432_drome Cdk9 CDK9 Dmel\CG5179 P-TEF P-TEFb PTefb cdk9 CG5179 Dmel_CG5179 DmP-TEFb protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MAHMSHMLQQPSGSTPSNVGSSSSRTMSLMEKQKYIEDYDFPYCDESNKYEKVAKIGQGTFGEVFKAREKKGNKKFVAMKKVLMDNEKEGFPITALREIRILQLLKHENVVNLIEICRTKATATNGYRSTFYLVFDFCEHDLAGLLSNMNVKFSLGEIKKVMQQLLNGLYYIHSNKILHRDMKAANVLITKHGILKLADFGLARAFSIPKNESKNRYTNRVVTLWYRPPELLLGDRNYGPPVDMWGAGCIMAEMWTRSPIMQGNTEQQQLTFISQLCGSFTPDVWPGVEELELYKSIELPKNQKRRVKERLRPYVKDQTGCDLLDKLLTLDPKKRIDADTALNHDFFWTDPMPSDLSKMLSQHLQSMFEYLAQPRRSNQMRNYHQQLTTMNQKPQDNSMIDRVW DIP protein O17432 original sequence version: 120 F028CD450C99D9D4 a8dyl7_drome pgc CG11296 CR32885 Dmel\CG32885 Pgc Pgc1 pgc-1 pgr CG32885 Dmel_CG32885 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MCDYQMEYSFIFEDSSCEGDASMASYDNGFESMWHQVREELQREREMNELCQVFQQNLSLSPPVIADRWRF DIP protein A8DYL7 original sequence version: 31 ccnt_drome Cyclin-T CycT CG6292 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSLLATPMPQAATASSSSSASAAASASGIPITANNNLPFEKDKIWYFSNDQLANSPSRRCGIKGDDELQYRQMTAYLIQEMGQRLQVSQLCINTAIVYMHRFYAFHSFTHFHRNSMASASLFLAAKVEEQPRKLEHVIRAANKCLPPTTEQNYAELAQELVFNENVLLQTLGFDVAIDHPHTHVVRTCQLVKACKDLAQTSYFLASNSLHLTSMCLQYRPTVVACFCIYLACKWSRWEIPQSTEGKHWFYYVDKTVSLDLLKQLTDEFIAIYEKSPARLKSKLNSIKAIAQGASNRTANSKDKPKEDWKITEMMKGYHSNITTPPELLNGNDSRDRDRDRERERERERDPSSLLPPPAMVPQQRRQDGGHQRSSSVSGVPGSSSSSSSSSHKMPNYPGGMPPDAHTDHKSKQPGYNNRMPSSHQRSSSSGLGSSGSGSQRSSSSSSSSSQQPGRPSMPVDYHKSSRGMPPVGVGMPPHGSHKMTSGSKPQQPQQQPVPHPSASNSSASGMSSKDKSQSNKMYPNAPPPYSNSAPQNPLMSRGGYPGASNGSQPPPPAGYGGHRSKSGSTVHGMPPFEQQLPYSQSQSYGHMQQQPVPQSQQQQMPPEASQHSLQSKNSLFSPEWPDIKKEPMSQSQPQPFNGLLPPPAPPGHDYKLNSHPRDKESPKKERLTPTKKDKHRPVMPPVGSGNSSSGSGSSKPMLPPHKKQIPHGGDLLTNPGESGSLKRPNEISGSQYGLNKLDEIDNSNMPREKLRKLDTTTGLPTYPNYEEKHTPLNMSNGIETTPDLVRSLLKESLCPSNASLLKPDALTMPGLKPPAELLEPMPAPATIKKEQGITPMTSLASGPAPMDLEVPTKQAGEIKEESSSKSEKKKKKDKHKHKEKDKSKDKTEKEERKKHKKDKQKDRSGSGGSKDSSLPNEPLKMVIKNPNGSLQAGASAPIKLKISKNKVEPNNYSAAAGLPGAIGYGLPPTTATTTSASIGAAAPVLPPYGAGGGGYSSSGGSSSGGSSKKKHSDRDRDKESKKNKSQDYAKYNGAGGGIFNPLGGAGAAPNMSGGMGAPMSTAVPPSMLLAPTGAVPPSAAGLAPPPMPVYNKK DIP protein O96433 original sequence version: 100 DIP protein O96433 original sequence version: 61 0E0E71B8A674FC5B pgc-cdk9-1 1 5 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 6 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association pgc-cdk9-2 4 5 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 6 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli physical association physical association cyct-cdk9-1 3 5 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly 7 unspecified role unspecified role bait bait Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range drome Drosophila melanogaster (Fruit fly) Fruit fly 6 unspecified role unspecified role prey prey drome Drosophila melanogaster (Fruit fly) Fruit fly physical association physical association pgc-cdk9-3 2 5 unspecified role unspecified role prey prey Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro 6 unspecified role unspecified role bait bait MBP-tag MBP-tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecolx Escherichia coli direct interaction direct interaction