DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature 2008 imex curation Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study predetermined predetermined participant predetermined Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. DIP original experiment: DIP-250322X Nature 2008 IMEx Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature 2008 imex curation Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. Only protein-protein interactions human Homo sapiens Human anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. DIP original experiment: DIP-250321X Nature 2008 IMEx Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. Nature 2008 imex curation Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. Only protein-protein interactions in vitro In vitro enzymatic study enzymatic study weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Ahel I., Ahel D., Matsusaka T., Clark AJ., Pines J., Boulton SJ., West SC. DIP original experiment: DIP-250323X Nature 2008 IMEx Poly(ADP-ribose)-binding zinc finger motifs in DNA repair/checkpoint proteins. parp1_human Poly [ADP-ribose] polymerase 1 PARP1 ADPRT PPOL NAD(+) ADP-ribosyltransferase 1 Poly[ADP-ribose] synthase 1 ADP-ribosyltransferase diphtheria toxin-like 1 DNA ADP-ribosyltransferase PARP1 Protein poly-ADP-ribosyltransferase PARP1 protein protein human Homo sapiens Human MAESSDKLYRVEYAKSGRASCKKCSESIPKDSLRMAIMVQSPMFDGKVPHWYHFSCFWKVGHSIRHPDVEVDGFSELRWDDQQKVKKTAEAGGVTGKGQDGIGSKAEKTLGDFAAEYAKSNRSTCKGCMEKIEKGQVRLSKKMVDPEKPQLGMIDRWYHPGCFVKNREELGFRPEYSASQLKGFSLLATEDKEALKKQLPGVKSEGKRKGDEVDGVDEVAKKKSKKEKDKDSKLEKALKAQNDLIWNIKDELKKVCSTNDLKELLIFNKQQVPSGESAILDRVADGMVFGALLPCEECSGQLVFKSDAYYCTGDVTAWTKCMVKTQTPNRKEWVTPKEFREISYLKKLKVKKQDRIFPPETSASVAATPPPSTASAPAAVNSSASADKPLSNMKILTLGKLSRNKDEVKAMIEKLGGKLTGTANKASLCISTKKEVEKMNKKMEEVKEANIRVVSEDFLQDVSASTKSLQELFLAHILSPWGAEVKAEPVEVVAPRGKSGAALSKKSKGQVKEEGINKSEKRMKLTLKGGAAVDPDSGLEHSAHVLEKGGKVFSATLGLVDIVKGTNSYYKLQLLEDDKENRYWIFRSWGRVGTVIGSNKLEQMPSKEDAIEHFMKLYEEKTGNAWHSKNFTKYPKKFYPLEIDYGQDEEAVKKLTVNPGTKSKLPKPVQDLIKMIFDVESMKKAMVEYEIDLQKMPLGKLSKRQIQAAYSILSEVQQAVSQGSSDSQILDLSNRFYTLIPHDFGMKKPPLLNNADSVQAKVEMLDNLLDIEVAYSLLRGGSDDSSKDPIDVNYEKLKTDIKVVDRDSEEAEIIRKYVKNTHATTHNAYDLEVIDIFKIEREGECQRYKPFKQLHNRRLLWHGSRTTNFAGILSQGLRIAPPEAPVTGYMFGKGIYFADMVSKSANYCHTSQGDPIGLILLGEVALGNMYELKHASHISKLPKGKHSVKGLGKTTPDPSANISLDGVDVPLGTGISSGVNDTSLLYNEYIVYDIAQVNLKYLLKLKFNFKTSLW DIP protein P09874 original sequence version: 189 DIP protein P09874 original sequence version: 183 DIP protein P09874 original sequence version: 166 DIP protein P09874 original sequence version: 117 DIP protein P09874 original sequence version: 143 DIP protein P09874 original sequence version: 136 DIP protein P09874 original sequence version: 137 DIP protein P09874 original sequence version: 147 DIP protein P09874 original sequence version: 193 DIP protein P09874 original sequence version: 202 DIP protein P09874 original sequence version: 171 DIP protein P09874 original sequence version: 153 DIP protein P09874 original sequence version: 175 6A5FC01EB91C046B q96ep1-1 E3 ubiquitin-protein ligase CHFR CHFR RNF196 Checkpoint with forkhead and RING finger domains protein RING finger protein 196 RING-type E3 ubiquitin transferase CHFR protein protein human Homo sapiens Human MERPEEGKQSPPPQPWGRLLRLGAEEGEPHVLLRKREWTIGRRRGCDLSFPSNKLVSGDHCRIVVDEKSGQVTLEDTSTSGTVINKLKVVKKQTCPLQTGDVIYLVYRKNEPEHNVAYLYESLSEKQGMTQESFEANKENVFHGTKDTSGAGAGRGADPRVPPSSPATQVCFEEPQPSTSTSDLFPTASASSTEPSPAGRERSSSCGSGGGGISPKGSGPSVASDEVSSFASALPDRKTASFSSLEPQDQEDLEPVKKKMRGDGDLDLNGQLLVAQPRRNAQTVHEDVRAAAGKPDKMEETLTCIICQDLLHDCVSLQPCMHTFCAACYSGWMERSSLCPTCRCPVERICKNHILNNLVEAYLIQHPDKSRSEEDVQSMDARNKITQDMLQPKVRRSFSDEEGSSEDLLELSDVDSESSDISQPYVVCRQCPEYRRQAAQPPHCPAPEGEPGAPQALGDAPSTSVSLTTAVQDYVCPLQGSHALCTCCFQPMPDRRAEREQDPRVAPQQCAVCLQPFCHLYWGCTRTGCYGCLAPFCELNLGDKCLDGVLNNNSYESDILKNYLATRGLTWKNMLTESLVALQRGVFLLSDYRVTGDTVLCYCCGLRSFRELTYQYRQNIPASELPVAVTSRPDCYWGRNCRTQVKAHHAMKFNHICEQTRFKN DIP protein Q96EP1-1 original sequence version: 62 aplf_human Aprataxin and PNK-like factor APLF C2orf13 PALF XIP1 Apurinic-apyrimidinic endonuclease APLF PNK and APTX-like FHA domain-containing protein XRCC1-interacting protein 1 protein protein human Homo sapiens Human MSGGFELQPRDGGPRVALAPGETVIGRGPLLGITDKRVSRRHAILEVAGGQLRIKPIHTNPCFYQSSEKSQLLPLKPNLWCYLNPGDSFSLLVDKYIFRILSIPSEVEMQCTLRNSQVLDEDNILNETPKSPVINLPHETTGASQLEGSTEIAKTQMTPTNSVSFLGENRDCNKQQPILAERKRILPTWMLAEHLSDQNLSVPAISGGNVIQGSGKEEICKDKSQLNTTQQGRRQLISSGSSENTSAEQDTGEECKNTDQEESTISSKEMPQSFSAITLSNTEMNNIKTNAQRNKLPIEELGKVSKHKIATKRTPHKEDEAMSCSENCSSAQGDSLQDESQGSHSESSSNPSNPETLHAKATDSVLQGSEGNKVKRTSCMYGANCYRKNPVHFQHFSHPGDSDYGGVQIVGQDETDDRPECPYGPSCYRKNPQHKIEYRHNTLPVRNVLDEDNDNVGQPNEYDLNDSFLDDEEEDYEPTDEDSDWEPGKEDEEKEDVEELLKEAKRFMKRK DIP protein Q8IW19 original sequence version: 82 DIP protein Q8IW19 original sequence version: 58 CBBF0096843298DA chfr-parp1-1 3 5 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 620-644 Region 620-644 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q96EP1-1:p.[Cys635Ala;Cys641Ala] C635A/C641A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C635A/C641A'. Wed May 02 14:41:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys635ala,cys641ala' Tue Jun 30 15:38:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys635Ala;Cys641Ala]' ecolx Escherichia coli 4 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction aplf-parp1-1 2 4 unspecified role unspecified role prey prey human Homo sapiens Human 6 unspecified role unspecified role bait bait Region 376-441 Region 376-441 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Flag-tag Flag-tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association aplf-parp1-2 1 4 enzyme enzyme unspecified role unspecified role ecolx Escherichia coli 6 enzyme target enzyme target substrate substrate unspecified role unspecified role Region 376-441 Region 376-441 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q8IW19:p.[Cys379Ala;Cys385Ala;Cys421Ala;Cys427Ala] C379A/C385A/C421A/C427A mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A certain certain sequence position certain certain certain sequence position certain C A This feature has been corrected as a result of our quality control procedures. The original label was 'C379A/C385A/C421A/C427A'. Wed May 02 16:45:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys379ala,cys385ala,cys421ala,cys427ala' Tue Jun 30 17:17:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys379Ala;Cys385Ala;Cys421Ala;Cys427Ala]' ecolx Escherichia coli direct interaction direct interaction