DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu The structure of a DnaB-family replicative helicase and its interactions with primase. The structure of a DnaB-family replicative helicase and its interactions with primase. Nature structural & molecular biology 2008 imex curation Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. DIP original experiment: DIP-113697X Nature structural & molecular biology 2008 IMEx The structure of a DnaB-family replicative helicase and its interactions with primase. The structure of a DnaB-family replicative helicase and its interactions with primase. The structure of a DnaB-family replicative helicase and its interactions with primase. Nature structural & molecular biology 2008 imex curation Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. Only protein-protein interactions in vitro In vitro comig non denat gel comigration in non denaturing gel electrophoresis comig non denat gel weight by comassie molecular weight estimation by coomasie staining weight by comassie Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. DIP original experiment: DIP-113699X Nature structural & molecular biology 2008 IMEx The structure of a DnaB-family replicative helicase and its interactions with primase. The structure of a DnaB-family replicative helicase and its interactions with primase. The structure of a DnaB-family replicative helicase and its interactions with primase. Nature structural & molecular biology 2008 imex curation Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. Only protein-protein interactions ecolx Escherichia coli x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Wang G., Klein MG., Tokonzaba E., Zhang Y., Holden LG., Chen XS. DIP original experiment: DIP-113698X Nature structural & molecular biology 2008 IMEx The structure of a DnaB-family replicative helicase and its interactions with primase. dnag_bacsu DNA primase dnaE BSU25210 dnaG protein protein bacsu Bacillus subtilis MGNRIPDEIVDQVQKSADIVEVIGDYVQLKKQGRNYFGLCPFHGESTPSFSVSPDKQIFHCFGCGAGGNVFSFLRQMEGYSFAESVSHLADKYQIDFPDDITVHSGARPESSGEQKMAEAHELLKKFYHHLLINTKEGQEALDYLLSRGFTKELINEFQIGYALDSWDFITKFLVKRGFSEAQMEKAGLLIRREDGSGYFDRFRNRVMFPIHDHHGAVVAFSGRALGSQQPKYMNSPETPLFHKSKLLYNFYKARLHIRKQERAVLFEGFADVISAVSSDVKESIATMGTSLTDDHVKILRRNVEEIILCYDSDKAGYEATLKASELLQKKGCKVRVAMIPDGLDPDDYIKKFGGEKFKNDIIDASVTVMAFKMQYFRKGKNLSDEGDRLAYIKDVLKEISTLSGSLEQEVYVKQLASEFSLSQESLTEQLSVFSKQNKPADNSGETKTRRAHLTTKARQKRLRPAYENAERLLLAHMLRDRSVIKKVIDRVGFQFNIDEHRALAAYLYAFYEEGAELTPQHLMARVTDDHISQLLSDILMLQVNQELSEAELSDYVKKVLNQRNWSMIKEKEAERAEAERQKDFLRAASLAQEIVTLNRSLK 525D8631845C14AA 525D8631845C14AA q38152_bpspp DNA helicase protein protein bpspp Bacillus phage SPP1 Bacteriophage SPP1 MNPEEKTVMLYNEYAEQAVLGSILTEPELIKECPLTPEHFSPGKHFNIYFTMQDLDRKGQSVDFTSIAARVGEKLPQLGGFGYLTELAASVASTTTFKQHCQTVSEYFQKRKAISIAQQIIENVNESDDGPVKPIQEAVSELMEIEASGTDDDDGSIDEALVTVYEEIESADGNITGVPSGFTELDRMTYGYKRRNFVLIAARPSMGKTAFALKQAKNMSDNDDVVNLHSLEMGKKENIKRLIVTAGSINAQKIKAARRDFASEDWGKLSMAIGEISNSNINIFDKAGQSVNYIWSKTRQTKRKNPGKRVIVMIDYLQLLEPAKANDSRTNQISQISRDLKKMARELDVVVIALSQLSRQVEQRQDKRPMLSDLRESGQLEQDADIIEFLYRDDYYDKESESKNIVEVIIAKHRDGPVGTVSLAFIKEYGNFVNLERRFDDR DIP protein Q38152 original sequence version: 43 q38152_bpspp-1 1 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction q38152_bpspp-2 3 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction dnag-q38152_bpspp-1 2 4 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli 5 unspecified role unspecified role unspecified role unspecified role ecolx Escherichia coli direct interaction direct interaction