DIP Database of Interacting Proteins 611 Young Drive East; Los Angeles CA 90095; USA http://dip.doe-mbi.ucla.edu/ dip@mbi.ucla.edu Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Structure (London, England : 1993) 2007 imex curation Eto M., Kitazawa T., Matsuzawa F., Aikawa S., Kirkbride JA., Isozumi N., Nishimura Y., Brautigan DL., Ohki SY. Only protein-protein interactions in vitro In vitro pull down pull down predetermined predetermined participant predetermined Eto M., Kitazawa T., Matsuzawa F., Aikawa S., Kirkbride JA., Isozumi N., Nishimura Y., Brautigan DL., Ohki SY. DIP original experiment: DIP-132713X Structure (London, England : 1993) 2007 IMEx Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. Structure (London, England : 1993) 2007 imex curation Eto M., Kitazawa T., Matsuzawa F., Aikawa S., Kirkbride JA., Isozumi N., Nishimura Y., Brautigan DL., Ohki SY. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Eto M., Kitazawa T., Matsuzawa F., Aikawa S., Kirkbride JA., Isozumi N., Nishimura Y., Brautigan DL., Ohki SY. DIP original experiment: DIP-132712X Structure (London, England : 1993) 2007 IMEx Phosphorylation-induced conformational switching of CPI-17 produces a potent myosin phosphatase inhibitor. pp14a_pig Protein phosphatase 1 regulatory subunit 14A CPI17 17 kDa PKC-potentiated inhibitory protein of PP1 Protein kinase C-potentiated inhibitor protein of 17 kDa protein protein pig Sus scrofa (Pig) Pig MAAQRLGKRVLSKLQSPSRARGPGGSPGGLQKRHARVTVKYDRRELQRRLDVEKWIDGRLEELYRGREADMPDEVNIDELLELESEEERSRKIQGLLKSCTNPTENFVQELLVKLRGLHKQPGLRQPSPSGDGSLSPRQDRARTAPP DIP protein O18734 original sequence version: 54 mypt1_human Protein phosphatase 1 regulatory subunit 12A PPP1R12A MBS MYPT1 Myosin phosphatase-targeting subunit 1 Protein phosphatase myosin-binding subunit protein protein human Homo sapiens Human MKMADAKQKRNEQLKRWIGSETDLEPPVVKRQKTKVKFDDGAVFLAACSSGDTDEVLKLLHRGADINYANVDGLTALHQACIDDNVDMVKFLVENGANINQPDNEGWIPLHAAASCGYLDIAEFLIGQGAHVGAVNSEGDTPLDIAEEEAMEELLQNEVNRQGVDIEAARKEEERIMLRDARQWLNSGHINDVRHAKSGGTALHVAAAKGYTEVLKLLIQAGYDVNIKDYDGWTPLHAAAHWGKEEACRILVDNLCDMEMVNKVGQTAFDVADEDILGYLEELQKKQNLLHSEKRDKKSPLIESTANMDNNQSQKTFKNKETLIIEPEKNASRIESLEQEKVDEEEEGKKDESSCSSEEDEEDDSESEAETDKTKPLASVTNANTSSTQAAPVAVTTPTVSSGQATPTSPIKKFPTTATKISPKEEERKDESPATWRLGLRKTGSYGALAEITASKEGQKEKDTAGVTRSASSPRLSSSLDNKEKEKDSKGTRLAYVAPTIPRRLASTSDIEEKENRDSSSLRTSSSYTRRKWEDDLKKNSSVNEGSTYHKSCSFGRRQDDLISSSVPSTTSTPTVTSAAGLQKSLLSSTSTTTKITTGSSSAGTQSSTSNRLWAEDSTEKEKDSVPTAVTIPVAPTVVNAAASTTTLTTTTAGTVSSTTEVRERRRSYLTPVRDEESESQRKARSRQARQSRRSTQGVTLTDLQEAEKTIGRSRSTRTREQENEEKEKEEKEKQDKEKQEEKKESETSREDEYKQKYSRTYDETYQRYRPVSTSSSTTPSSSLSTMSSSLYASSQLNRPNSLVGITSAYSRGITKENEREGEKREEEKEGEDKSQPKSIRERRRPREKRRSTGVSFWTQDSDENEQEQQSDTEEGSNKKETQTDSISRYETSSTSAGDRYDSLLGRSGSYSYLEERKPYSSRLEKDDSTDFKKLYEQILAENEKLKAQLHDTNMELTDLKLQLEKATQRQERFADRSLLEMEKRERRALERRISEMEEELKMLPDLKADNQRLKDENGALIRVISKLSK DIP protein O14974 original sequence version: 95 DIP protein O14974 original sequence version: 141 EA43E9BFF5DA08FF ppp1r12a-cpi17-1 2 3 unspecified role unspecified role prey prey ecolx Escherichia coli 4 unspecified role unspecified role bait bait Binding region 1-19 Binding region 1-19 binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain in vitro In vitro direct interaction direct interaction cpi17-ppp1r12a-1 1 3 unspecified role unspecified role bait bait ecolx Escherichia coli 4 unspecified role unspecified role prey prey Binding region 1-300 Binding region 1-300 sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain Myc-tag Myc-tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range human Homo sapiens Human physical association physical association